LOCUS       sequence-20117-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, 
            Maruyama M, Maeda M, Yamanaka S
  TITLE     The homeoprotein Nanog is required for maintenance of pluripotency 
            in mouse epiblast and ES cells.
  JOURNAL   Cell. 2003 May 30. 113(5):631-42.
  PUBMED    12787504
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2003 
            May 30. 113(5):631-42."
COMMENT     Sequence Label: pDEST17-Nanog
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..28
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    38..62
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtcgtactac catcaccatc accatcacct cgaatcaaca agtttgtaca aaaaagcagg
       61 ctctgacatg agtgtgggtc ttcctggtcc ccacagtttg cctagttctg aggaagcatc
      121 gaattctggg aacgcctcat caatgcctgc agtttttcat cccgagaact attcttgctt
      181 acaagggtct gctactgaga tgctctgcac agaggctgcc tctcctcgcc cttcctctga
      241 agacctgcct cttcaaggca gccctgattc ttctaccagt cccaaacaaa agctctcaag
      301 tcctgaggct gacaagggcc ctgaggagga ggagaacaag gtccttgcca ggaagcagaa
      361 gatgcggact gtgttctctc aggcccagct gtgtgcactc aaggacaggt ttcagaagca
      421 gaagtacctc agcctccagc agatgcaaga actctcctcc attctgaacc tgagctataa
      481 gcaggttaag acctggtttc aaaaccaaag gatgaagtgc aagcggtggc agaaaaacca
      541 gtggttgaag actagcaatg gtctgattca gaagggctca gcaccagtgg agtatcccag
      601 catccattgc agctatcccc agggctatct ggtgaacgca tctggaagcc tttccatgtg
      661 gggcagccag acttgg
//
