LOCUS       sequence-201172-        8602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off 
            all-in-one vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Gu X, Orozco JM, Saxton RA, Condon KJ, Liu GY, Krawczyk PA, Scaria 
            SM, Harper JW, Gygi SP, Sabatini DM
  TITLE     SAMTOR is an S-adenosylmethionine sensor for the mTORC1 pathway.
  JOURNAL   Science. 2017 Nov 10;358(6364):813-818. doi: 
            10.1126/science.aao3265.
  PUBMED    29123071
REFERENCE   2  (bases 1 to 8602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2017-11-10- 10"; volume: "358"; 
            issue: "6364"; pages: "813-818"
COMMENT     Sequence Label: pCW57.1-MAT2A
FEATURES             Location/Qualifiers
     source          1..8602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        172..486
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    216..234
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    251..269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    287..305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    323..341
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    358..376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    394..412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     459..483
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1699..2209
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2128..2146
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2219..2611
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             2621..2674
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2675..3292,3293..3298,3299..3334,3335..3337,3338..3373,
                     3374..3376,3377..3412,3413..3421)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3461..3481)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3879..3890)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4095..4328
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4400..4521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4437..4456)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4491..4510
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4561..4696
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4696..4767
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4768..4836,4837..5628)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4986..5005)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5799..6387
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6288..6307
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6541..6558
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6675..6696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6711..6716,6717..6734,6735..6741)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6749..6765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6754..6776
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6773..6789
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6773..6789
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6808..6828
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6810..6828
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6856..7082
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7083..7263
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7310..7435
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7928..8161
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8346..8390
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccact ttggccgcga atcgatatgt cgagtttact
      181 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg
      241 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
      301 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
      361 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
      421 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
      481 atcgcctgga gaattggcta gcaccatgaa cggacagctc aacggcttcc acgaggcgtt
      541 catcgaggag ggcacattcc ttttcacctc agagtcggtc ggggaaggcc acccagataa
      601 gatttgtgac caaatcagtg atgctgtcct tgatgcccac cttcagcaag atcctgatgc
      661 caaagtagct tgtgaaactg ttgctaaaac tggaatgatc cttcttgctg gggaaattac
      721 atccagagct gctgttgact accagaaagt ggttcgtgaa gctgttaaac acattggata
      781 tgatgattct tccaaaggtt ttgactacaa gacttgtaac gtgctggtag ccttggagca
      841 acagtcacca gatattgctc aaggtgttca tcttgacaga aatgaagaag acattggtgc
      901 tggagaccag ggcttaatgt ttggctatgc cactgatgaa actgaggagt gtatgccttt
      961 aaccattgtc ttggcacaca agctaaatgc caaactggca gaactacgcc gtaatggcac
     1021 tttgccttgg ttacgccctg attctaaaac tcaagttact gtgcagtata tgcaggatcg
     1081 aggtgctgtg cttcccatca gagtccacac aattgttata tctgttcagc atgatgaaga
     1141 ggtttgtctt gatgaaatga gggatgccct aaaggagaaa gtcatcaaag cagttgtgcc
     1201 tgcgaaatac cttgatgagg atacaatcta ccacctacag ccaagtggca gatttgttat
     1261 tggtgggcct cagggtgatg ctggtttgac tggacgcaaa atcattgtgg acacttatgg
     1321 cggttggggt gctcatggag gaggtgcctt ttcaggaaag gattatacca aggtcgaccg
     1381 ttcagctgct tatgctgctc gttgggtggc aaaatccctt gttaaaggag gtctgtgccg
     1441 gagagttctt gttcaggtct cttatgctat tggagtttct catccattat ctatctccat
     1501 tttccattat ggtacctctc agaagagtga gagagagcta ttagagattg tgaagaagaa
     1561 tttcgatctc cgccctgggg tcattgtcag ggatctggat ctgaagaagc caatttatca
     1621 gaggactgca gcctatggcc actttggtag ggacagcttc ccatgggaag tgcccaaaaa
     1681 gcttaaatat tgaggtccgg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc
     1741 gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc
     1801 tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg
     1861 ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc
     1921 gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg
     1981 ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc
     2041 agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt
     2101 agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac
     2161 gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagc aattcaccat
     2221 ggttcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
     2281 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
     2341 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
     2401 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
     2461 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
     2521 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
     2581 attgctgccc tctggttatg tgtgggaggg ccccggtgcc gaaggtagag gttctctcct
     2641 cacttgtggt gatgttgaag aaaaccctgg tccaatgtct agactggaca agagcaaagt
     2701 cataaactct gctctggaat tactcaatga agtcggtatc gaaggcctga cgacaaggaa
     2761 actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg
     2821 ggccctgctc gatgccctgg caatcgagat gctggacagg catcataccc acttctgccc
     2881 cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat tccgctgtgc
     2941 tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca
     3001 gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa
     3061 cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggatca
     3121 ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact
     3181 tctgagacaa gcaattgagc tgttcgacca tcagggagcc gaacctgcct tccttttcgg
     3241 cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccggc
     3301 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga
     3361 ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta
     3421 actaagtaag gatcgatcca agatatcgta ttcttaacta tgttgctcct tttacgctat
     3481 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3541 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     3601 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3661 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     3721 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3781 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     3841 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3901 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3961 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ttcgcctcgg cgtccggact
     4021 agaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4081 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     4141 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     4201 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     4261 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     4321 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     4381 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     4441 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4501 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     4561 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4621 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4681 ggcttttttg gaggcctttc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4741 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4801 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4861 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4921 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4981 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5041 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5101 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5161 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5221 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5281 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5341 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5401 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5461 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5521 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5581 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5641 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5701 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5761 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5821 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5881 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5941 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6001 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6061 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6121 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6181 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6241 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6301 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6361 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6421 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6481 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6541 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     6601 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     6661 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     6721 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     6781 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     6841 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     6901 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     6961 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     7021 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     7081 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7141 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7201 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7261 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     7321 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     7381 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     7441 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     7501 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     7561 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     7621 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     7681 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     7741 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     7801 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     7861 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     7921 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     7981 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     8041 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     8101 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     8161 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     8221 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     8281 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     8341 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     8401 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     8461 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     8521 caccattatc gtttcagacc cacctcccaa ccccgagggg acaattctcg acctcgagac
     8581 aaatggcagt attcatccac aa
//
