LOCUS       sequence-201179-        2980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mobius Assembly Level 2 Acceptor Vector Delta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Andreou AI, Nakayama N
  TITLE     Mobius Assembly: A versatile Golden-Gate framework towards universal
            DNA assembly.
  JOURNAL   PLoS One. 2018 Jan 2;13(1):e0189892. doi: 
            10.1371/journal.pone.0189892. eCollection 2018.
  PUBMED    29293531
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-01-2"; volume: "13(1):e0189892. doi"; pages: " 
            10.1371/journal.pone.0189892. eCollection 2018"
COMMENT     Sequence Label: pMA_Level 2-Delta
FEATURES             Location/Qualifiers
     source          1..2980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..58
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(82..99)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(253..841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(333..352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1024..1118)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1139..1798)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1713..1732
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1960..1978
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1982..2025)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        2060..2094
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2103..2114
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      2845..2916
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2932..2959
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
       61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct
     1021 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     1081 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     1141 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     1201 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     1261 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     1321 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     1381 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     1441 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     1501 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     1561 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     1621 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     1681 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     1741 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     1801 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     1861 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
     1921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
     1981 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcggtctca
     2041 cttgggagat atgcaggtgt ttacggctag ctcagtccta ggtatagtgc tagctactag
     2101 agaaagagga gaaatactaa atgcgtaaag gcgaagaact gttcacgggc gtagttccga
     2161 ttctggtcga gctggacggc gatgtgaacg gtcataagtt tagcgttcgc ggtgaaggtg
     2221 agggcgacgc gaccaacggc aaactgaccc tgaagttcat ctgcaccacc ggtaaactgc
     2281 cggtgccttg gccgaccttg gtgacgacgt tgacgtatgg cgtgcagtgt tttgcgcgtt
     2341 atccggacca catgaaacaa cacgatttct tcaaatctgc gatgccggag ggttacgtcc
     2401 aggagcgtac catttccttc aaggatgatg gctactacaa aactcgcgca gaggttaagt
     2461 ttgaaggtga cacgctggtc aatcgtatcg aattgaaggg tatcgacttt aaagaggatg
     2521 gtaacattct gggccataaa ctggagtata acttcaacag ccataatgtt tacattacgg
     2581 cagacaagca aaagaacggc atcaaggcca atttcaagat tcgccacaat gttgaggacg
     2641 gtagcgtcca actggccgac cattaccagc agaacacccc aattggtgac ggtccggttt
     2701 tgctgccgga taatcactat ctgagcaccc aaagcgtgct gagcaaagat ccgaacgaaa
     2761 aacgtgatca catggtcctg ctggaatttg tgaccgctgc gggcatcacc cacggtatgg
     2821 acgagctgta taagtaatga gcttcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2881 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     2941 tcgggtgggc ctttctgcgc acctgcatat acccctgcag
//
