LOCUS       sequence-201195-        6084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of APOBEC3C DS-EP mutant in mammalian cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Jaguva Vasudevan AA, Hofmann H, Willbold D, Haussinger D, Koenig BW,
            Munk C
  TITLE     Enhancing the Catalytic Deamination Activity of APOBEC3C Is 
            Insufficient to Inhibit Vif-Deficient HIV-1.
  JOURNAL   J Mol Biol. 2017 Apr 21;429(8):1171-1191. doi: 
            10.1016/j.jmb.2017.03.015. Epub 2017 Mar 16.
  PUBMED    28315663
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2017.03.015"; journalName: "J Mol Biol"; date: 
            "2017-04-21- 21"; volume: "429"; issue: "8"; pages: "1171-1191"
COMMENT     Sequence Label: pcDNA3.1 human A3C.DS-EP
FEATURES             Location/Qualifiers
     source          1..6084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(32..51)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        223..602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        603..806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     757..777
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     851..870
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        851..869
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1551..1577
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1664..1681)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1670..1894
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1940..2368
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2027..2046)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2237..2258
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2377..2397)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2382..2711
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2562..2697
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2624..2643
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2778..3572
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2832..2851)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3442..3461
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3746..3867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3783..3802)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3837..3856
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3916..3932)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3916..3932)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3929..3951)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3940..3956
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3964..3970,3971..3988,3989..3994))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4009..4030
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4147..4164)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4317..4905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4397..4416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5076..5867,5868..5936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5699..5718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5937..6041)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
       61 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
      121 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
      181 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
      241 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      301 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      361 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      421 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      481 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      541 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      601 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      661 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      721 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      781 ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact gcttactggc
      841 ttatcgaaat taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa
      901 gcttggtacc gagctcggat ccactagtcc agtgtggtgg aattcgccct ttaagcggaa
      961 ttctatctaa gaggctgaac atgaatccac agatcagaaa cccgatgaag gcaatgtatc
     1021 caggcacatt ctacttccaa tttaaaaacc tatgggaagc caacgatcgg aacgaaactt
     1081 ggctgtgctt caccgtggaa ggtataaagc gccgctcagt tgtctcctgg aagacgggcg
     1141 tcttccgaaa ccaggtggat cctgagaccc attgtcatgc agaaaggtgc ttcctctctt
     1201 ggttctgcga cgacatactg tctcctaaca caaagtacca ggtcacctgg tacacatctt
     1261 ggagcccttg cccagagtgt gcaggggagg tggccgagtt cctggccagg cacagcaacg
     1321 tgaatctcac catcttcacc gcccgcctct actacttcca gtatccatgt taccaggagg
     1381 ggctccgcag cctgagtcag gaaggggtcg ctgtggagat catggactat gaagatttta
     1441 aatattgttg ggaaaacttt gtgtacaatg ataatgagcc attcaagcct tggaagggat
     1501 taaaaaccaa ctttcgactt ctgaaaagaa ggctacggga gagtctccag tatccatacg
     1561 atgttccaga ttacgcttga ctcgactaag ggcgaattct gcagatatcc agcacagtgg
     1621 cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     1681 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     1741 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     1801 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     1861 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     1921 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     1981 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2041 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     2101 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     2161 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2221 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     2281 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     2341 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     2401 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2461 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2521 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     2581 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     2641 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     2701 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     2761 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2821 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2881 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     2941 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3001 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3061 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3121 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3181 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3241 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3301 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3361 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3421 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3481 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3541 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3601 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3661 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3721 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     3781 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3841 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     3901 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3961 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4021 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4081 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4141 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4201 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4261 gtgagcaaaa ggcagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4321 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4381 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4441 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4501 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4561 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4621 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4681 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4741 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4801 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4861 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4921 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4981 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5041 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5101 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5161 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5221 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5281 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5341 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5401 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5461 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5521 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5581 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5641 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5701 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5761 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5821 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5881 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5941 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6001 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6061 ccacctgacg tcgacggatc ggga
//
