LOCUS       sequence-201222-        9236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of HA tagged ARHGAP29.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Qiao Y, Chen J, Lim YB, Finch-Edmondson ML, Seshachalam VP, Qin L, 
            Jiang T, Low BC, Singh H, Lim CT, Sudol M
  TITLE     YAP Regulates Actin Dynamics through ARHGAP29 and Promotes 
            Metastasis.
  JOURNAL   Cell Rep. 2017 May 23;19(8):1495-1502. doi: 
            10.1016/j.celrep.2017.04.075.
  PUBMED    28538170
REFERENCE   2  (bases 1 to 9236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.04"; journalName: "Cell Rep"; date: 
            "2017-05-23- 23"; volume: "19"; issue: "8"; pages: "1495-1502"
COMMENT     Sequence Label: pcDNA3.1-HA-ARHGAP29
FEATURES             Location/Qualifiers
     source          1..9236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..518
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        519..722
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     673..693
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     767..786
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        767..785
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             830..856
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4731..4748)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4737..4961
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5007..5435
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5094..5113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5304..5325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5444..5464)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5449..5778
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5629..5764
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5691..5710
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5845..6639
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5899..5918)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6509..6528
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6813..6934
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6850..6869)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6904..6923
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6983..6999)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6983..6999)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6996..7018)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7007..7023
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7031..7037,7038..7055,7056..7061))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7076..7097
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7214..7231)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7385..7973)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7465..7484)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8144..8935,8936..9004))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8767..8786
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9005..9109)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9184..9203)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
       61 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
      121 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      181 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      241 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      301 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      361 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      421 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      481 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      541 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      601 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      661 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      721 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
      781 atagggagac ccaagctggc tagcgtttaa acttaagctt gccaccatgt acccatacga
      841 tgttccagat tacgctggta ccgagctcgg atccatgatt gctcacaaac agaaaaagac
      901 aaagaaaaaa cgtgcttggg catcaggtca actctctact gatattacaa cttctgaaat
      961 ggggctcaag tccttaagtt ccaactctat ttttgatccg gattacatca aggagttggt
     1021 gaatgatatc aggaagttct cccacatgtt actatatttg aaagaagcca tattttcaga
     1081 ctgttttaaa gaagttattc atatacgtct agaggaactg ctccgtgttt taaagtctat
     1141 aatgaataaa catcagaacc tcaattctgt tgatcttcaa aatgctgcag aaatgctcac
     1201 tgcaaaagtg aaagctgtga acttcacaga agttaatgaa gaaaacaaaa acgatctctt
     1261 ccaggaagtg ttttcttcta ttgaaacttt ggcatttacc tttggaaata tccttacaaa
     1321 cttccttatg ggagatgtag gcaatgattc attattgcga ctgcctgttt ctcgagaaac
     1381 taagtcgttt gaaaatgttt ctgtggaatc agtggactca tccagtgaaa aaggaaattt
     1441 ttccccttta gaactagaca acgtgctgtt aaagaacact gactctatcg agctggcttt
     1501 gtcatatgct aaaacttggt caaaatatac taagaacata gtttcatggg ttgaaaaaaa
     1561 gcttaacttg gaattggagt ccactagaaa tatggtcaag ttggcagagg caactagaac
     1621 taacattgga attcaggagt tcatgccact gcagtctctg tttactaatg ctcttcttaa
     1681 tgatatagaa agcagtcacc ttttacaaca aacaattgca gctctccagg ctaacaaatt
     1741 tgtgcagcct ctacttggaa ggaaaaatga aatggaaaaa caaaggaaag aaataaaaga
     1801 gctttggaaa caggagcaaa ataaaatgct tgaagcagag aatgctctca aaaaggcaaa
     1861 attattatgc atgcaacgtc aagatgaata tgagaaagca aagtcttcca tgtttcgtgc
     1921 agaagaggag catctgtctt caagtggcgg attagcaaaa aatctcaaca agcaactaga
     1981 aaaaaagcga aggttggaag aggaggctct ccaaaaagta gaagaagcaa atgaacttta
     2041 caaagtttgt gtgacaaatg ttgaagaaag aagaaatgat ctagaaaata ccaaaagaga
     2101 aattttagca caactccgga cacttgtttt ccagtgtgat cttaccctta aagctgtaac
     2161 agttaacctc ttccacatgc agcatctgca ggctgcttcc cttgcagaca gtttacagtc
     2221 tctctgtgat agtgccaaac tctatgaccc aggccaagag tacagtgaat ttgtcaaggc
     2281 cacaaattca actgaagaag aaaaagttga tggaaatgta aataaacatt taaatagttc
     2341 ccaaccttca ggatttggac ctgccaactc tttagaggat gttgtacgcc ttcctgacag
     2401 ttctaataaa attgaagagg acagatgctc taacagtgca gatataacag gtccttcctt
     2461 tataagatca tggacatttg ggatgtttag tgattctgag agcactggag ggagcagcga
     2521 atctagatct ctggattcag aatctataag tccaggagac tttcatcgaa aacttccacg
     2581 aacaccatcc agtggaacta tgtcctctgc agatgatcta gatgaaagag agccaccttc
     2641 cccttcagaa actggaccca attcccttgg aacatttaag aaaacattga tgtcaaaggc
     2701 agctctcaca cacaagtttc gcaaattgag atcccccacg aaatgtaggg attgtgaagg
     2761 cattgtagtg ttccaaggtg ttgaatgtga agagtgtctc cttgtttgtc atcgaaagtg
     2821 tttggaaaat ttagtcatta tttgtggtca tcagaaactt ccaggaaaaa tacacttatt
     2881 tggagcagaa ttcacacaag ttgcaaaaaa ggaaccagat ggtatccctt ttatactcaa
     2941 aatatgtgcc tcagagattg aaaatagagc tttgtgtcta cagggaattt atcgtgtgtg
     3001 tggaaacaaa ataaaaactg aaaaattgtg tcaagctttg gaaaatggaa tgcacttggt
     3061 agatatttca gaatttagtt cacatgatat ctgtgacgtc ttgaaattat accttcggca
     3121 gctcccagaa ccatttattt tatttcgatt gtacaaggaa tttatagacc ttgcaaaaga
     3181 gatccaacat gtaaatgaag aacaagagac aaaaaagaat agtcttgaag acaaaaaatg
     3241 gccaaatatg tgtatagaaa taaaccgaat tcttctaaaa agcaaagacc ttctaagaca
     3301 attgccagca tcaaatttta acagtcttca tttccttata gtacatctaa agcgggtagt
     3361 agatcatgca gaagaaaaca agatgaactc caaaaacttg ggggtgatat ttggaccaag
     3421 tctcattagg ccaaggccca caactgctcc tatcaccatc tcctcccttg cagagtattc
     3481 aaatcaagca cgcttggtag agtttctcat tacttactca cagaagatct tcgatgggtc
     3541 cctacaacca caagatgtta tgtgtagcat aggtgttgtt gatcaaggct gttttccaaa
     3601 gcctctgtta tcaccagaag aaagagacat tgaacgttcc atgaagtcac tatttttttc
     3661 ttcaaaggaa gatatccata cttcagagag tgaaagcaaa atttttgaac gagctacatc
     3721 atttgaggaa tcagaacgca agcaaaatgc gttaggaaaa tgtgatgcat gtctcagtga
     3781 caaagcacag ttgcttctag accaagaggc tgaatcagca tcccaaaaga tagaagatgg
     3841 taaaacccct aagccacttt ctctgaaatc tgataggtca acaaacaatg tggagaggca
     3901 tactccaagg accaagatta gacctgtaag tttgcctgta gatagactac ttcttgcaag
     3961 tcctcctaat gagagaaatg gcagaaatat gggaaatgta aatttagaca agttttgcaa
     4021 gaatcctgcc tttgaaggag ttaatagaaa agacgctgct actactgttt gttccaaatt
     4081 taatggcttt gaccagcaaa ctctacagaa aattcaggac aaacagtatg aacaaaacag
     4141 cctaactgcc aagactacaa tgatcatgcc cagtgcactc caggaaaaag gagtgacaac
     4201 aagcctccag attagtgggg accattctat caatgccact caacccagta agccatatgc
     4261 agagccagtc aggtcagtga gagaggcatc tgagagacgg tcttcagatt cctaccctct
     4321 cgctcctgtc agagcaccca gaacactgca gcctcaacat tggacaacat tttataaacc
     4381 acatgctccc atcatcagta tcagggggaa tgaggagaag ccagcttcac cctcagcagc
     4441 agtgcctcct ggcacagatc acgatcccca cggtctcgtg gtgaagtcaa tgccagaccc
     4501 agacaaagca tcagcttgtc ctgggcaagc aactggtcaa cctaaagaag actctgagga
     4561 gcttggcttg cctgatgtga atccaatgtg tcagagacca aggctaaaac gaatgcaaca
     4621 gtttgaagac ctcgaaggtg aaattccaca atttgtgtag gaattctgca gatatccagc
     4681 acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     4741 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     4801 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     4861 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     4921 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     4981 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     5041 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5101 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5161 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5221 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5281 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5341 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5401 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     5461 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5521 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5581 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5641 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5701 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5761 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     5821 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     5881 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5941 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     6001 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     6061 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6121 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6181 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6241 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6301 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6361 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6421 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6481 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6541 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6601 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     6661 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     6721 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     6781 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     6841 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6901 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     6961 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7021 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7081 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7141 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7201 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7261 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7321 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7381 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7441 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7501 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7561 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7621 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7681 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7741 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7801 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7861 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7921 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7981 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8041 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8101 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8161 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8221 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8281 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8341 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8401 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8461 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8521 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8581 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8641 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8701 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8761 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8821 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8881 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8941 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9001 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9061 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9121 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     9181 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtg
//
