LOCUS       sequence-201234-       13290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation Lentiviral vector for Tet-based inducible shRNA or 
            cDNA expression, constitutive Neomycin resistance gene 
            coexpression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13290)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 13290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pSLIK-Neo
FEATURES             Location/Qualifiers
     source          1..13290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2377..2501
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2526..2531,2532..2549,2550..2556)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2610..3269
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2676..2695)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3611..3916
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3795..3814
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3957..4081)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4131..5342
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5179..5198
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5401..6018,6019..6024,6025..6060,6061..6063,6064..6099,
                     6100..6102,6103..6138,6139..6147)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6301..6763
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6357..6374)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6584..6603
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6779..7573
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6779..7573
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6833..6852)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7443..7462
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7741..8329
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7794..7814)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8212..8223)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8332..8348)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8333..8349)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8858..9038
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9064..9081)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9070..9294
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9340..9768
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9427..9446)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9637..9658
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9777..9797)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9782..10111
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9962..10097
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10024..10043
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10159..10206
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10225..10599
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10729..10850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10766..10785)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10820..10839
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10899..10915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10899..10915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10912..10934)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10923..10939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10947..10953,10954..10971,10972..10977))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10992..11013
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11130..11147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11301..11889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11381..11400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12060..12851,12852..12920))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12683..12702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12921..13025)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13100..13119)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttctagacag ttatcaacaa gtttgtacaa aaaagctgaa
     2401 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     2461 actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc
     2521 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt
     2581 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     2641 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     2701 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     2761 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     2821 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     2881 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     2941 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     3001 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     3061 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     3121 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     3181 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     3241 actgcgatga gtggcagggc ggggcgtaaa gatctggatc cggcttacta aaagccagat
     3301 aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata
     3361 cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag
     3421 cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca
     3481 accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa
     3541 gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg
     3601 ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
     3661 tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc
     3721 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
     3781 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg
     3841 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
     3901 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata
     3961 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
     4021 ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg
     4081 ttgataacat ctcgagccat aaagatggtt aattaaccca cccaagatct ggcctccgcg
     4141 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     4201 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     4261 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     4321 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     4381 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     4441 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     4501 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     4561 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     4621 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     4681 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     4741 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     4801 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     4861 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     4921 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     4981 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     5041 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     5101 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     5161 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     5221 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     5281 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     5341 acgaagcttg gtaccgagct cggatctcca ccccgtaccg gtcctgcagt cgaattcacc
     5401 atgtctagac tggacaagag caaagtcata aacggagctc tggaattact caatggtgtc
     5461 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
     5521 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
     5581 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
     5641 aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
     5701 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
     5761 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
     5821 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
     5881 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag
     5941 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
     6001 ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc
     6061 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     6121 tttgaccttg acatgctccc cgggtaacta agtaaggatc cgcggccgca ctagaggaat
     6181 tccgcccctc tccccccccc ccctctcccc taacgttact ggccgaagcc gcttggaata
     6241 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     6301 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     6361 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     6421 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     6481 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     6541 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     6601 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     6661 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     6721 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttacc ggtccaccat
     6781 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     6841 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     6901 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     6961 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7021 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7081 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7141 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     7201 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     7261 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     7321 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     7381 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     7441 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     7501 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     7561 cgagttcttc tgatgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca
     7621 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     7681 ataaaatgaa tgcaattgtt gttgtttagt ccctcccaat tcgatatcaa gcttatcgat
     7741 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7801 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7861 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7921 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7981 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8041 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8101 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     8161 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8221 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     8281 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     8341 cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     8401 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     8461 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     8521 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     8581 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     8641 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     8701 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     8761 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     8821 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     8881 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8941 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9001 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     9061 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     9121 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     9181 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     9241 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     9301 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     9361 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     9421 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     9481 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     9541 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     9601 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     9661 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     9721 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     9781 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9841 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     9901 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9961 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    10021 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
    10081 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
    10141 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
    10201 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
    10261 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
    10321 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
    10381 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
    10441 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
    10501 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
    10561 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
    10621 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
    10681 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
    10741 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    10801 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
    10861 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
    10921 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    10981 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    11041 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    11101 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    11161 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    11221 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    11281 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    11341 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    11401 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    11461 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    11521 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    11581 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    11641 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    11701 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    11761 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    11821 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11881 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    11941 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    12001 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    12061 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    12121 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    12181 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    12241 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    12301 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    12361 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    12421 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    12481 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    12541 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    12601 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    12661 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    12721 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    12781 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    12841 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    12901 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    12961 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    13021 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
    13081 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
    13141 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
    13201 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
    13261 ttcgcgatgt acgggccaga tatacgcgtt
//
