LOCUS       sequence-201240-        8476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human codon optimized NgAgo; pCDNA3 vector backbone, mammalian 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   O'Geen H, Ren C, Coggins NB, Bates SL, Segal DJ
  TITLE     Unexpected binding behaviors of bacterial Argonautes in human cells 
            cast doubts on their use as targetable gene regulators.
  JOURNAL   PLoS One. 2018 Mar 27;13(3):e0193818. doi: 
            10.1371/journal.pone.0193818. eCollection 2018.
  PUBMED    29584750
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-03-27"; volume: "13(3):e0193818. doi"; pages: " 
            10.1371/journal.pone.0193818. eCollection 2018"
COMMENT     Sequence Label: pCDNA-hNgAgo
FEATURES             Location/Qualifiers
     source          1..8476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        126..505
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        506..709
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     660..680
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     706..730
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             827..847
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             866..931
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1016..3676
                     /codon_start=1
                     /product="Argonaute protein from Natronobacterium gregoryi 
                     (Gao et al., 2016)"
                     /label=NgAgo
                     /note="reportedly a DNA-guided endonuclease that does not 
                     require a PAM site, although this interpretation has been 
                     challenged (Cyranoski, 2016)"
                     /translation="MTVIDLDSTTTADELTSGHTYDISVTLTGVYDNTDEQHPRMSLAF
                     EQDNGERRYITLWKNTTPKDVFTYDYATGSTYIFTNIDYEVKDGYENLTATYQTTVENA
                     TAQEVGTTDEDETFAGGEPLDHHLDDALNETPDDAETESDSGHVMTSFASRDQLPEWTL
                     HTYTLTATDGAKTDTEYARRTLAYTVRQELYTDHDAAPVATDGLMLLTPEPLGETPLDL
                     DCGVRVEADETRTLDYTTAKDRLLARELVEEGLKRSLWDDYLVRGIDEVLSKEPVLTCD
                     EFDLHERYDLSVEVGHSGRAYLHINFRHRFVPKLTLADIDDDNIYPGLRVKTTYRPRRG
                     HIVWGLRDECATDSLNTLGNQSVVAYHRNNQTPINTDLLDAIEAADRRVVETRRQGHGD
                     DAVSFPQELLAVEPNTHQIKQFASDGFHQQARSKTRLSASRCSEKAQAFAERLDPVRLN
                     GSTVEFSSEFFTGNNEQQLRLLYENGESVLTFRDGARGAHPDETFSKGIVNPPESFEVA
                     VVLPEQQADTCKAQWDTMADLLNQAGAPPTRSETVQYDAFSSPESISLNVAGAIDPSEV
                     DAAFVVLPPDQEGFADLASPTETYDELKKALANMGIYSQMAYFDRFRDAKIFYTRNVAL
                     GLLAAAGGVAFTTEHAMPGDADMFIGIDVSRSYPEDGASGQINIAATATAVYKDGTILG
                     HSSTRPQLGEKLQSTDVRDIMKNAILGYQQVTGESPTHIVIHRDGFMNEDLDPATEFLN
                     EQGVEYDIVEIRKQPQTRLLAVSDVQYDTPVKSIAAINQNEPRATVATFGAPEYLATRD
                     GGGLPRPIQIERVAGETDIETLTRQVYLLSQSHIQVHNSTARLPITTAYADQASTHATK
                     GYLVQTGAFESNVGFL"
     CDS             3752..3799
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3818..3874
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(3938..3957)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3982..4030
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4232..4660
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4319..4338)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4529..4550
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4669..4689)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4674..5003
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4854..4989
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4916..4935
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5070..5864
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5124..5143)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5734..5753
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6040..6161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6077..6096)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6131..6150
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6210..6226)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6210..6226)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6223..6245)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6234..6250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6258..6264,6265..6282,6283..6288))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6303..6324
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6441..6458)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6612..7200)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6692..6711)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7371..8162,8163..8231))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7994..8013
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8232..8336)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8411..8430)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
       61 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
      121 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      181 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      241 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      301 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      361 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      421 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      481 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      541 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      601 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      661 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
      721 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      781 ccgatccagc ctccggactc tagaggatcg aacccttgcc accatgccca agaagaagag
      841 gaaggtggga ggctccggag gaagcgacta taaggaccac gacggagact acaaggatca
      901 tgatattgat tacaaagacg atgacgataa gggtggcggg tccggcggtg gatccggtac
      961 cggagggagt ggtggaagcg gcggttctgg tggctcaggc ggcagcggcc ggccaatgac
     1021 agtgatagat ctggattcaa caacaaccgc agacgagctt acgtctggac atacttatga
     1081 tatcagcgtc actcttaccg gggtctacga taacactgac gagcagcacc cccgcatgtc
     1141 ccttgctttc gaacaagaca atggcgagcg acgctacata accctctgga agaacacaac
     1201 tcccaaggac gtcttcactt acgattatgc cacaggcagt acgtatattt tcactaatat
     1261 tgactacgag gtcaaggacg gttatgaaaa ccttaccgcc acctaccaaa caacggttga
     1321 aaatgctaca gctcaagaag tcggcaccac ggatgaagac gaaacatttg ccgggggcga
     1381 accattggat caccacctgg atgatgcact gaacgagacc cccgatgatg cggaaacgga
     1441 aagtgattca ggccatgtaa tgacatcatt cgcgtcacgc gatcagctgc cggagtggac
     1501 attgcacaca tacactctca cggccacgga tggggctaaa acagatacgg aatacgcgag
     1561 aagaactctg gcttacactg ttcggcaaga actttatacc gaccacgacg cagcaccagt
     1621 ggccaccgac ggcttgatgc ttctcactcc tgagcctctg ggagagaccc ctcttgatct
     1681 tgattgtggc gtgcgcgttg aggctgacga gacaaggacg ttggattaca caaccgcaaa
     1741 agatagactt ctcgctcggg aacttgtgga ggaggggttg aaacggtctc tttgggacga
     1801 ttacctcgtg aggggcatag atgaagtcct gtcaaaggag cccgtgttga cttgtgatga
     1861 gtttgacctg cacgagcgct atgacctgtc tgtcgaggtt ggtcattctg gccgggctta
     1921 tcttcacatt aacttccgcc accgcttcgt accgaagctc acgcttgcag acatagatga
     1981 cgacaacatt tatccggggt tgcgggtgaa aacaacctac agacctcgcc gaggccatat
     2041 agtctggggt ctccgcgacg aatgtgcgac cgactccctt aatactttgg gtaatcagtc
     2101 agtagtagct tatcaccgca ataaccagac accaatcaat accgaccttc tcgatgctat
     2161 tgaagccgca gaccgcagag tcgtcgaaac gcgcaggcaa ggacatgggg atgacgccgt
     2221 atcattcccc caggagcttc tcgcagtaga acctaataca catcaaataa agcagttcgc
     2281 ctccgacggg tttcatcagc aggctaggtc taagacccga ctcagtgcat caagatgttc
     2341 agaaaaagca caagcatttg cggagcggct tgaccctgtg cgattgaacg ggtctacggt
     2401 ggaattctct tctgagttct tcacggggaa caacgagcaa cagctccggt tgttgtacga
     2461 aaacggcgaa tccgtgttga cctttcggga tggtgccagg ggggcccatc ccgacgaaac
     2521 atttagtaag ggaatagtca atccaccaga gtcatttgag gtagcagtcg tgctgcctga
     2581 acagcaggct gacacatgta aagcccagtg ggacactatg gcagacctct tgaatcaggc
     2641 gggcgctcct cctactcgat cagagacggt tcaatatgat gcgttctctt ctcctgaaag
     2701 tatttctctc aatgtagccg gtgcgataga cccgtcagaa gtcgatgcag cgttcgtcgt
     2761 tctgccccct gatcaggagg gtttcgctga tcttgcttct ccaaccgaaa cctatgacga
     2821 actgaaaaaa gcacttgcga acatgggcat ttattctcaa atggcatact tcgatcgctt
     2881 tcgggacgct aagattttct acaccaggaa cgttgccttg ggtttgctgg cagcagcggg
     2941 cggcgtcgct tttacgacag aacacgcgat gcctggagat gcggacatgt ttattggaat
     3001 tgatgtgagc cgaagctatc cggaagatgg ggcgtcaggt cagattaata tagcggctac
     3061 cgcgacggcc gtctataagg acggtacgat attgggtcac tcttccacga ggcctcaact
     3121 cggagaaaag ctccaatcaa ctgacgtcag agatataatg aagaatgcga tattgggcta
     3181 tcagcaagtg acgggtgaat cccctaccca tattgttatc catcgcgacg gatttatgaa
     3241 cgaagatttg gatcccgcga ctgagtttct caacgaacag ggtgtggagt atgacattgt
     3301 ggagattagg aaacaacccc aaacacggct cttggccgtg tcagacgtcc agtacgatac
     3361 gcctgtgaag tcaattgcag ccatcaatca gaacgaaccc cgggcgaccg ttgcgacctt
     3421 tggagctccg gagtatcttg cgactcgcga tggcggaggg ttgccgagac cgatacagat
     3481 agagagggtc gccggcgaaa cggatataga aacgctcaca cgccaagtat accttctttc
     3541 tcaatctcat atccaggtgc acaactcaac ggctcgcttg cccataacga cggcatacgc
     3601 tgaccaggcg tcaacgcatg cgacgaaggg ttatctggta caaacggggg cgttcgagag
     3661 taacgtcggt tttttgggtg gtagtggagg ttcaggagga tccgggggga gcggagggag
     3721 cgctagcggt ggaggctccg gaggtggatc taaaaggccg gcggccacga aaaaggccgg
     3781 tcaggcaaaa aagaaaaagg gtggtagtgg aagcggagct actaacttca gcctgctgaa
     3841 gcaggctgga gacgtggagg agaaccctgg acctgcggcc gcatgaaagg gttcgatccc
     3901 taccggttag taatgagttt aaacggggga ggctaactga aacacggaag gagacaatac
     3961 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg
     4021 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     4081 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     4141 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcagat
     4201 ctgcgcagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4321 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4381 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4441 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4501 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4561 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4621 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     4681 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4741 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4801 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4861 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4921 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     4981 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     5041 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     5101 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5161 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5221 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     5281 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5341 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     5401 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5461 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     5521 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     5581 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     5641 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     5701 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     5761 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5821 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     5881 cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     5941 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     6001 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     6061 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6121 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     6181 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6241 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6301 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6361 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6421 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6481 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6541 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6601 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6661 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6721 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6781 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6841 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6901 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6961 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7021 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7081 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7141 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7201 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7261 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7321 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7381 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7441 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7501 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7561 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7621 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7681 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7741 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7801 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7861 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7921 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7981 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8041 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8101 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8161 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8221 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8281 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8341 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     8401 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     8461 gtgtgttgga ggtcgc
//
