LOCUS       sequence-201243-       10110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split-BioID plasmid: Expresses N-terminally tagged 
            CBir[257-321]-Cdc25C S216A and N-terminally tagged 
            NBir[2-256]-14-3-3e.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild
            K, Bethune J
  TITLE     Split-BioID a conditional proteomics approach to monitor the 
            composition of spatiotemporally defined protein complexes.
  JOURNAL   Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690.
  PUBMED    28585547
REFERENCE   2  (bases 1 to 10110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-6"; volume: "8:15690. doi"; pages: " 
            10.1038/ncomms15690"
COMMENT     Sequence Label: pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e
FEATURES             Location/Qualifiers
     source          1..10110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             519..988
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1053..1410
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1821..1843
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1859..1875
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1928..1961
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    2069..2203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2106..2125)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3986..4015)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(4113..4150)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(4167..4185,4186..4208,4209..4227,4228..4250,4251..4269,
                     4270..4292,4293..4311,4312..4334,4335..4353,4354..4376,
                     4377..4395,4396..4418,4419..4437)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4452..4490
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4602..4625
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    6352..6399
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(7483..7500)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7483..7499)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7492..7514)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7708..7727)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7827..7849
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7887..7905)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7973..8077
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8078..8146,8147..8938)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8296..8315)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9109..9697
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9598..9617
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9851..9868
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9985..10006
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10021..10026,10027..10044,10045..10051)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10059..10075
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10064..10086
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10083..10099
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10083..10099
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 caagctagct ttgctcttag gagtttccta atacatccca aactcaaata tataaagcat
       61 ttgacttgtt ctatgcccta gggggcgggg ggaagctaag ccagcttttt ttaacattta
      121 aaatgttaat tccattttaa atgcacagat gtttttattt cataagggtt tcaatgtgca
      181 tgaatgctgc aatattcctg ttaccaaagc tagtataaat aaaaatagat aaacgtggaa
      241 attacttaga gtttctgtca ttaacgtttc cttcctcagt tgacaacata aatgcgctgc
      301 tgagaagcca gtttgcatct gtcaggatca atttcccatt atgccagtca tattaattac
      361 tagtcaatta gttgattttt atttttgaca tatacatgtg aaagacccca cctgtaggtt
      421 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat
      481 agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat
      541 atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata
      601 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
      661 tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg
      721 gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc
      781 tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac
      841 tcggcgcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc
      901 tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga
      961 ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct
     1021 gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta tctgtgtctg
     1081 tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac
     1141 tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc cggccgcaac
     1201 cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg agtcctaaaa
     1261 tcccgatcgt ttaggactct ttggtgcacc ccccttagag gagggatatg tggttctggt
     1321 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggga
     1381 ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac
     1441 tgtgtttctg tatttgtctg aaaatatggg cccgggctag cctgttacca ctcccttaag
     1501 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1561 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1621 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1681 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1741 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1801 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1861 ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga tccgaagttc
     1921 ctattccgaa gttcctattc ttcaaatagt ataggaactt cagatcataa tcagccatac
     1981 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     2041 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     2101 ataaggcaat agcatcacaa atttcacaaa taaggcattt ttttcactgc attctagttt
     2161 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctcaaatc cctcggaagc
     2221 tgcgcctgtc ttaggttgga gtgatacatt tttatcactt ttacccgtct ttggattagg
     2281 cagtagctct gacggccctc ctgtcttagg ttagtgaaaa atgtcactct cttacccgtc
     2341 attggctgtc cagcttagct cgcaggggag gtggtctgga tccctaacgc gtctgatttt
     2401 cgtcttccac gtcctgcagc gcttctttat tctgctcttc accgtcaccc tgcatgtctg
     2461 aagtccatag tgtcagatta tcacgtaaca actgcatgat aagtgtagag tccttatagc
     2521 tttcttcact cagcgtatcc agttctgcaa ttgcatcatc aaaagctgct tttgccaacc
     2581 tgcaggcacg gtcaggggaa ttaagaattt cgtagtagaa tacggaaaaa ttgagagcaa
     2641 gacctaagcg aataggatgc gttggtggaa gttctgtcat tgcaatatca ctagcagctt
     2701 tataagccac taggctgttc tccgcagcct ccttcctgtc gtttcctgtg gcaaattctg
     2761 ccagatacct gtggtagtcc cctttcattt tataatagaa aaccttggac tcgccagtgt
     2821 tagctgctgg aatgaggtgt ttgtccagta catccagaat gtcacaacag attaacttta
     2881 gctcagtctc aaccatttgc cgatattccc gaatcatttt tagcttgtct tctcctccct
     2941 tgttttcttc tttctgttca atgctgctga ttattctcca ggaggctctt ctagctccaa
     3001 tcacattctt atatgcaaca gataggaggt ttctttcttc aactgtcagc tccacatcca
     3061 tccctgctac tttcttcatt gaccccacca tttcgtcgta tcgctcagcc tgctcggcca
     3121 gcttcgcctg gtacaccaga tcctctcgat catccatatc gatgccccgg gaggcgtagg
     3181 agatctggcc gccgccggag gagccgccgc catgtttctc ctgctcgaac agctccagag
     3241 cggctctcag ctctctgatc agcatggcgg ccagggtgtt ccggtccagg ttgatgccag
     3301 cctcctgcag ggtgatccag ccctggttca ccacgctctc ctccactctt ctcatggcca
     3361 tgttgattcc ggctccgatc acgatctgag cggcatcgcc tgtcttgcct gtcagctcca
     3421 ccaggatgcc agccagcttg cggtcctgga ggtacaggtc attaggccac ttcactctca
     3481 ccttgtcggc tcccagcttt ctcagcacct cggccatcac gattccgatc accagactca
     3541 gtccgatggc agcagcaggt ccctgctcca gtctccagaa catggacagg tacaggttgg
     3601 ctccgaatgg gctgaaccac ttccgtcctc tgcctcctct gccagcctgc tggtactcgg
     3661 cgatgcaggc gtcgccggac ttcagctctc cgattctgtc cagcaggtac tgattggtgg
     3721 agtcgatcac aggcagcacg gccacgcttc cgccatccag ctgtcccagg atctgcttgg
     3781 cgttcagcag ctggataggc tcaggcaggc tgtagccctt gccaggcacg gtgaacacgt
     3841 ccactcccca gtctctcagt gtctggatgt gcttgttgat ggcggctctg ctcattccca
     3901 gggtctctcc cagctgctcg ccagagtgga actcgccgtt ggccagcagg gcgatcagct
     3961 tcaggggcac ggtgttgtcc ttgtcgagat cctcttctga gatgagtttt tgttccatct
     4021 cgaccgcgga ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca gcgtggatgg
     4081 cgtctccagg cgatctgacg gttcactaaa cgagctctgc ttatataggc ctcccaccgt
     4141 acacgcctac aagcttcttt cacttttctc tatcactgat agggagtggt aaactcgact
     4201 ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac
     4261 tgatagggag tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac
     4321 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc
     4381 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
     4441 gtaaactcga gggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga
     4501 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
     4561 accgatccag cctccgcgga attcggccgg cctcaattat ggactacaag gacgacgatg
     4621 acaagattaa cggactggct ccttacctga gcagatggga gaagctggac aacttcatca
     4681 acagacctgt gaagctgatc atcggcgaca aggaaatctt cggcatctcc agaggaatcg
     4741 acaagcaggg agctctgctg ctggagcagg acggaatcat caagccctgg atgggcggag
     4801 aaatctccct gagaagcgca gagaagctcg aggcgaacca gatctcctac gcctcccggg
     4861 gcggcggctc ctccggcggc ggcgtttgtt tcatgtcctc tacggaactc ttctcatcca
     4921 caagagagga aggaagctct ggctcaggac ccagttttag gtctaatcaa aggaaaatgt
     4981 taaacctgct cctggagaga gacacttcct ttaccgtctg tccagatgtc cctagaactc
     5041 cagtgggcaa atttcttggt gattctgcaa acctaagcat tttgtctgga ggaaccccaa
     5101 aatgttgcct cgatctttcg aatcttagca gtggggagat aactgccact cagcttacca
     5161 cttctgcaga ccttgatgaa actggtcacc tggattcttc aggacttcag gaagtgcatt
     5221 tagctgggat gaatcatgac cagcacctaa tgaaatgtag cccagcacag cttctttgta
     5281 gcactccgaa tggtttggac cgtggccata gaaagagaga tgcaatgtgt agttcatctg
     5341 caaataaaga aaatgacaat ggaaacttgg tggacagtga aatgaaatat ttgggcagtc
     5401 ccattactac tgttccaaaa ttggataaaa atccaaacct aggagaagac caggcagaag
     5461 agatttcaga tgaattaatg gagttttccc tgaaagatca agaagcaaag gtgagcagaa
     5521 gtggcctata tcgctccccg gcgatgccag agaacttgaa caggccaaga ctgaagcagg
     5581 tggaaaaatt caaggacaac acaataccag ataaagttaa aaaaaagtat ttttctggcc
     5641 aaggaaagct caggaagggc ttatgtttaa agaagacagt ctctctgtgt gacattacta
     5701 tcactcagat gctggaggaa gattctaacc aggggcacct gattggtgat ttttccaagg
     5761 tatgtgcgct gccaaccgtg tcagggaaac accaagatct gaagtatgtc aacccagaaa
     5821 cagtggctgc cttactgtcg gggaagttcc agggtctgat tgagaagttt tatgtcattg
     5881 attgtcgcta tccatatgag tatctgggag gacacatcca gggagcctta aacttatata
     5941 gtcaggaaga actgtttaac ttctttctga agaagcccat cgtccctttg gacacccaga
     6001 agagaataat catcgtgttc cactgtgaat tctcctcaga gaggggcccc cgaatgtgcc
     6061 gctgtctgcg tgaagaggac aggtctctga accagtatcc tgcattgtac tacccagagc
     6121 tatatatcct taaaggcggc tacagagact tctttccaga atatatggaa ctgtgtgaac
     6181 cacagagcta ctgccctatg catcatcagg accacaagac tgagttgctg aggtgtcgaa
     6241 gccagagcaa agtgcaggaa ggggagcggc agctgcggga gcagattgcc cttctggtga
     6301 aggacatgag cccattaatt aaatagcgat taataacgag gatctggccg cgaagttcct
     6361 attccgaagt tcctattctc tagaaagtat aggaacttca gatccggatt agtccaattt
     6421 gttaaagaca ggatatcagt ggtccaggct ctagttttga ctcaacaata tcaccagctg
     6481 aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct ccagaaaaag
     6541 gggggaatga aagaccccac ctgtaggttt ggcaagctag ctcgacttct gttcgcgcgc
     6601 ttctgctccc cgagctcgag cccacaaccc ctcactcggg gcgccagtcc tccgattgac
     6661 tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg
     6721 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt
     6781 tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt acattaaatg
     6841 gccatagtac ttaaagttac attggcttcc ttgaaataaa catggagtat tcagaatgtg
     6901 tcataaatat ttctaatttt aagatagtat ctccattggc tttctacttt ttcttttatt
     6961 tttttttgtc ctctgtcttc catttgttgt tgttgttgtt tgtttgtttg tttgttggtt
     7021 ggttggttaa ttttttttta aagatcctac actatagttc aagctagact attagctact
     7081 ctgtaaccca gggtgacctt gaagtcatgg gtagcctgct gttttagcct tcccacatct
     7141 aagattacag gtatgagcta tcatttttgg tatattgatt gattgattga ttgatgtgtg
     7201 tgtgtgtgat tgtgtttgtg tgtgtgattg tgtatatgtg tgtatggttg tgtgtgattg
     7261 tgtgtatgta tgtttgtgtg tgattgtgtg tgtgtgattg tgcatgtgtg tgtgtgtgat
     7321 tgtgtttatg tgtatgattg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     7381 tgtgttgtgt atatatattt atggtagtga gaggcaacgc tccggctcag gtgtcaggtt
     7441 ggtttttgag acagagtctt tcacttagct tggaattaat tcactggccg tcgttttaca
     7501 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     7561 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     7621 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     7681 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     7741 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     7801 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     7861 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     7921 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     7981 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     8041 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     8101 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     8161 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     8221 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     8281 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     8341 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     8401 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8461 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8521 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8581 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8641 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8701 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8761 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8821 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8881 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8941 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     9001 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     9061 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     9121 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     9181 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9241 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9301 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9361 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9421 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9481 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9541 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9601 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9661 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9721 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9781 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9841 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9901 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     9961 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    10021 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    10081 cacaggaaac agctatgacc atgattacgc
//
