LOCUS       sequence-201244-        8385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase lentivirus based enhancer assay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8385)
  TITLE     pLS-SV40-mP-Rluc
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLS-SV40-mP-Rluc
FEATURES             Location/Qualifiers
     source          1..8385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1686..1919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2104..2148
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2446..2563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3058..3249
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3081..3101)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3333..3364
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      3404..3413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3410..4345
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    complement(4385..4418)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4474..5062
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4527..4547)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4945..4956)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5065..5081)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5066..5082)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6391..6571
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6633..7221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6713..6732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7392..8183,8184..8252))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8015..8034
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8253..8357)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccggg cccggtgctt tgctctgagc cagcccacca
     2641 gtttggaatg actccttttt atgacttgaa ttttcaagta taaagtctag tgctaaattt
     2701 aatttgaaca actgtatagt ttttgctggt tgggggaagg aaaaaaaatg gtggcagtgt
     2761 ttttttcaga attagaagtg aaatgaaaat tgttgtgtgt gaggatttct aatgacatgt
     2821 ggtggttgca tactgagtga agccggtgag cattctgcca tgtcaccccc tcgtgctcag
     2881 taatgtactt tacagaaatc ctaaactcaa aagattgata taaaccatgc ttcttgtgta
     2941 tatccggtct cttctctggg tagtctcact cagcctgcat ttctgccagg gcccgctcta
     3001 gagcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg
     3061 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     3121 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     3181 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3241 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3301 tccgccccat ggctgtctag acctgcaggc actagagggt atataatgga agctcgactt
     3361 ccagcttggc aatccggtac tgtaccggtg tactgttggt aaagccacca tggcttccaa
     3421 ggtgtacgac cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg
     3481 caagcaaatg aacgtgctgg actccttcat caactactat gattccgaga agcacgccga
     3541 gaacgccgtg atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt
     3601 gcctcacatc gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc
     3661 cggcaagagc gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg
     3721 gttcgagctg ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg
     3781 tctggccttt cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga
     3841 gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc
     3901 cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac
     3961 catgctccca agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga
     4021 gccattcaag gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc
     4081 tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct
     4141 tcgggccagc gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa
     4201 cgctattgtc gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct
     4261 ccacttcagc caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga
     4321 gcgcgtgctg aagaacgagc agtaattcta gagtcggggc ggccggaatt cgtcgaggga
     4381 cctaataact tcgtatagca tacattatac gaagttatac atgtttaagg gttccggttc
     4441 cactaggtac aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     4501 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4561 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4621 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4681 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4741 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4801 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4861 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     4921 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4981 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     5041 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca
     5101 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     5161 acaagaggag gaggaggtgg gttttccagt cacacctcag gtaccaagca tggggtaaag
     5221 tactgttctc atcacatcat atcaaggtta tataccatca atattgccac agatgttact
     5281 tagcctttta atatttctct aatttagtgt atatgcaatg atagttctct gatttctgag
     5341 attgagtttc tcatgtgtaa tgattattta gagtttctct ttcatctgtt caaatttttg
     5401 tctagtttta ttttttactg atttgtaaga cttcttttta taatctgcat attacaattc
     5461 tctttactgg ggtgttgcaa atattttctg tcattctatg gcctgacttt tcttaatggt
     5521 tttttaattt taaaaataag tcttaatatt catgcaatct aattaacaat cttttctttg
     5581 tggttaggac tttgagtcat aagaaatttt tctctacact gaagtcatga tggcatgctt
     5641 ctatattatt ttctaaaaga tttaaagttt tgccttctcc atttagactt ataattcact
     5701 ggaatttttt tgtgtgtatg gtatgacata tgggttccct tttatttttt acatataaat
     5761 atatttccct gtttttctaa aaaagaaaaa gatcatcatt ttcccattgt aaaatgccat
     5821 atttttttca taggtcactt acatatatca atgggtctgt ttctgagctc tactctattt
     5881 tatcagcctc actgtctatc cccacacatc tcatgctttg ctctaaatct tgatatttag
     5941 tggaacattc tttcccattt tgttctacaa gaatattttt gttattgtct tttgggcttc
     6001 tatatacatt ttagaatgag gttggcaagt tctgtagatc ttagccactt tttaaaagaa
     6061 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     6121 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     6181 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     6241 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     6301 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     6361 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     6421 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6481 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6541 cccttttagt cagtgtggaa aatctctagc agcatgtgag caaaaggcca gcaaaaggcc
     6601 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6661 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6721 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6781 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6841 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6901 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6961 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7021 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7081 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7141 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7201 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7261 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7321 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7381 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7441 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7501 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7561 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7621 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7681 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7741 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7801 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7861 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7921 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7981 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8041 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8101 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8161 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8221 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8281 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8341 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//
