LOCUS       sequence-201245-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase lentivirus based enhancer assay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  TITLE     pLS-SV40-mP-Rluc
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLS-SV40-mP-Rluc
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        104..295
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(127..147)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        379..410
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      450..459
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gaaaaaggga cgatctctcg ctgcatttct gccagggccc gctctagagc gcagcaccat
       61 ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc ggaaagaacc
      121 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
      181 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
      241 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
      301 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
      361 gtctagacct gcaggcacta gagggtatat aatggaagct cgacttccag cttggcaatc
      421 cggtactgta ccggtgtact gttggtaaag ccaccatggc ttccaaggtg tacgaccccg
      481 agcaacgcaa acgcatgatc actgggcctc agtggtgggc tcgctgcaag caaatgaacg
      541 tgctggactc cttcatcaac tactatgatt ccgagaagca cgccgagaac gccgtgattt
      601 ttctgcatgg taacgctgcc tccagctacc tgtggaggca cgtcgtgcct cacatcgagc
      661 ccgtggctag atgcatcatc cctgatctga tcggaatggg taagtccggc aagagcggga
      721 atggctcata tcgcctcctg gatcactaca agtacctcac cgcttggttc gagctgctga
      781 accttccaaa gaaaatcatc tttgtgggcc acgactgggg ggcttgtctg gcctttcact
      841 actcctacga gcaccaagac aagatcaagg ccatcgtcca tgctgagagt gtcgtggacg
      901 tgatcgagtc ctgggacgag tggcctgaca tcgaggagga tatcgctctg atcaagagcg
      961 aagagggcga gaaatggtgc ttgagaataa cttcttcgtc
//
