LOCUS       sequence-201252-        8974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module 3 - GAL4 synthetic promoter (with eight binding sites) drives
            the expression of mKate2 transcripts containing miR1-Bs(Pe).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN431 (FuGW-G8p-mKate2-1Pe )
FEATURES             Location/Qualifiers
     source          1..8974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             147..191
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    489..606
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      944..953
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             950..1663
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    1715..2303
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1768..1788)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2186..2197)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2306..2322)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2307..2323)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2828..3008
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3034..3051)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3040..3264
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3310..3738
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3397..3416)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3607..3628
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3747..3767)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3752..4081
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3932..4067
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3994..4013
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4129..4176
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4195..4569
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4699..4820
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4736..4755)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4790..4809
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4869..4885)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4869..4885)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4882..4904)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4893..4909
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4917..4923,4924..4941,4942..4947))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4962..4983
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5100..5117)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5271..5859)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5351..5370)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6030..6821,6822..6890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6653..6672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6891..6995)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7070..7089)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7261..7640
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7642..7840
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7795..7815
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7858..8038
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8085..8210
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8703..8936
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
       61 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
      121 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
      181 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
      241 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
      301 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
      361 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
      421 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
      481 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      541 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      601 aattttcggg tttattacag ggacagcaga gatccagttt ggttaatgga tcctcgatag
      661 gtaccgagtt tctagacgga gtactgtcct ccgagcggag tactgtcctc cgactcgagc
      721 ggagtactgt cctccgatcg gagtactgtc ctccgcgaat tccggagtac tgtcctccga
      781 agacgctaga cggagtactg tcctccgagc ggagtactgt cctccgactc gagcggagta
      841 ctgtcctccg gctagcgggg ggctataaaa gggggtgggg gcgttcgtcc tcactctaga
      901 tctgcgatct aagtaagctt ggcattccgg tactgttggt aaagccacca tggtgtctaa
      961 gggcgaagag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa
     1021 caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
     1081 catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
     1141 cagcttcatg tacggcagca aaaccttcat caaccacacc cagggcatcc ccgacttctt
     1201 taagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
     1261 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
     1321 gatcagaggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg
     1381 ggaggcctcc accgagatgc tgtaccccgc tgacggcggc ctggaaggca gaagcgacat
     1441 ggccctgaag ctcgtgggcg ggggccacct gatctgcaac ttgaagacca catacagatc
     1501 caagaaaccc gctaagaacc tcaagatgcc cggcgtctac tatgtggaca gaagactgga
     1561 aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag
     1621 atactgcgac ctccctagca aactggggca caaacttaat taggcaccgc ttgaagtctt
     1681 taattaaata ccggtcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     1741 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     1801 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     1861 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     1921 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     1981 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2041 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2101 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     2161 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2221 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2281 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
     2341 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     2401 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     2461 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     2521 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     2581 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     2641 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     2701 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     2761 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     2821 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2881 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2941 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3001 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     3061 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3121 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3181 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3241 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     3301 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3361 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3421 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3481 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3541 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3601 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3661 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3721 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3781 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3841 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3901 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3961 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4021 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     4081 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     4141 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     4201 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     4261 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     4321 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     4381 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     4441 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     4501 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     4561 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     4621 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     4681 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4741 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4801 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     4861 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4921 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     4981 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5041 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5101 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5161 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5221 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5281 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5341 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5401 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5461 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5521 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5581 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5641 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5701 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5761 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5821 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5881 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5941 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6001 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6061 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6901 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6961 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7021 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     7081 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     7141 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     7201 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     7261 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     7321 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     7381 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     7441 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7501 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7561 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7621 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     7681 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     7741 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     7801 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     7861 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7921 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7981 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8041 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     8101 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     8161 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     8221 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     8281 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     8341 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     8401 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     8461 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     8521 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     8581 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     8641 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     8701 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     8761 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     8821 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     8881 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     8941 atttggggtt gctctggaaa actcatttgc acca
//
