LOCUS       sequence-201258-        9077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module 3 - GAL4 synthetic promoter( with fourteen binding sites) 
            drives mKate2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN432 (FuGW-G14p-mKate2)
FEATURES             Location/Qualifiers
     source          1..9077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             153..197
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    495..612
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      1076..1085
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1082..1795
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    1824..2412
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1877..1897)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2295..2306)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2415..2431)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2416..2432)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2937..3117
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3143..3160)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3149..3373
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3419..3847
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3506..3525)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3716..3737
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3856..3876)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3861..4190
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4041..4176
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4103..4122
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4238..4285
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4304..4678
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4808..4929
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4845..4864)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4899..4918
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4978..4994)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4978..4994)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4991..5013)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5002..5018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5026..5032,5033..5050,5051..5056))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5071..5092
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5209..5226)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5380..5968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5460..5479)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6139..6930,6931..6999))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6762..6781
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7000..7104)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7179..7198)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7370..7749
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7751..7949
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7904..7924
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7967..8147
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8194..8319
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8812..9045
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
       61 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
      121 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
      181 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
      241 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
      301 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
      361 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
      421 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
      481 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
      541 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
      601 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt aatggatcct
      661 cgataggtac cgagtttcta gacggagtac tgtcctccga gcggagtact gtcctccgac
      721 tcgagcggag tactgtcctc cgatcggagt actgtcctcc gcgaattccg gagtactgtc
      781 ctccgaagac gctagacgga gtactgtcct ccgagcggag tactgtcctc cgactcgagc
      841 ggagtactgt cctccggcgg agtactgtcc tccgagcgga gtactgtcct ccgactcgag
      901 cggagtactg tcctccggct agacggagta ctgtcctccg agcggagtac tgtcctccga
      961 ctcgagcgga gtactgtcct ccgctagcgg ggggctataa aagggggtgg gggcgttcgt
     1021 cctcactcta gatctgcgat ctaagtaagc ttggcattcc ggtactgttg gtaaagccac
     1081 catggtgtct aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga
     1141 gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga
     1201 gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga
     1261 catcctggct accagcttca tgtacggcag caaaaccttc atcaaccaca cccagggcat
     1321 ccccgacttc tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
     1381 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     1441 ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa
     1501 aacactcggc tgggaggcct ccaccgagat gctgtacccc gctgacggcg gcctggaagg
     1561 cagaagcgac atggccctga agctcgtggg cgggggccac ctgatctgca acttgaagac
     1621 cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga
     1681 cagaagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt
     1741 ggctgtggcc agatactgcg acctccctag caaactgggg cacaaactta attgataaac
     1801 cggtcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     1861 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     1921 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     1981 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2041 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     2101 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     2161 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     2221 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     2281 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     2341 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     2401 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     2461 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     2521 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     2581 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     2641 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     2701 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     2761 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     2821 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     2881 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     2941 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     3001 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     3061 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     3121 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3181 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     3241 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3301 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     3361 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     3421 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3481 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3541 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3601 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3661 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3721 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3781 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3841 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3901 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3961 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4021 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4081 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     4141 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     4201 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     4261 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     4321 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     4381 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     4441 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     4501 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     4561 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     4621 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     4681 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     4741 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     4801 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4861 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4921 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     4981 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5041 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5101 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5161 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5221 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5281 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5341 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5401 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5461 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5521 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5581 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5641 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5701 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5761 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5821 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5881 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5941 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6001 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6061 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6121 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6181 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6241 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6301 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6361 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6421 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6481 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6541 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6601 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6661 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6721 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6781 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6841 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6901 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6961 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7021 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7081 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     7141 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     7201 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     7261 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     7321 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     7381 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     7441 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     7501 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     7561 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     7621 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     7681 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     7741 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     7801 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     7861 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     7921 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     7981 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8041 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8101 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     8161 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     8221 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     8281 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     8341 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     8401 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     8461 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8521 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     8581 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8641 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8701 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8761 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8821 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8881 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8941 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     9001 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     9061 ctctggaaaa ctcattt
//
