LOCUS       sequence-201260-        6581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes sequence of the murine H2-Kb allele.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Kelton W, Waindok AC, Pesch T, Pogson M, Ford K, Parola C, Reddy ST
  TITLE     Reprogramming MHC specificity by CRISPR-Cas9-assisted cassette 
            exchange.
  JOURNAL   Sci Rep. 2017 Apr 4;7:45775. doi: 10.1038/srep45775.
  PUBMED    28374766
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-04-4"; volume: "7:45775. doi"; pages: " 
            10.1038/srep45775"
COMMENT     Sequence Label: pUC19, containing H2-Kb c57/BL6 WK9
FEATURES             Location/Qualifiers
     source          1..6581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2946..2968
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3006..3024)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3092..3196
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3197..3265,3266..4057)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3415..3434)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4228..4816
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4717..4736
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4970..4987
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctctacact aggacggttc acctagtttc tagaattttc caaagaatac attctcacag
       61 atccctccct gtctgtgggg ttttgcaccc cttaacaacc caattctatc tattcctaca
      121 gtggtgaatg gtcacatgag cccttatggg ctaccctgga ggaatataaa tcgtggattt
      181 tcttctctct ctctctctcc ctctctttcc gttttttttt tttttttttt tttttttttt
      241 tcctagacgg ggtttctctg tatagccctg gctgtcctgg aactcacttt gtagaccagg
      301 ttggccttga actcagaaat ctgcctgcct ctggctcccg agtgctggga ttaaaggcat
      361 gcgccaccac tcctggcctt tcttttttct taactttttt tttggtgggg ggggggctta
      421 ttttgtttct agtcagcttt tgtctgcatt ggagtgatcc agtctctcca tgccctccta
      481 ttatcatttg tttcagtctc cacaggtgcc agggaaggct gctaacctgg ataattagac
      541 aagttaaatc agggttcgaa ttaaagaaga gattcctgtg agcttagact gtttcctgtc
      601 agaactaaac atgcagaagc ctcctgctct tcctctgccc cacaagttac agtgctccct
      661 cccgctagta aatcaggact tggactctga gaggcagggt cttctgcaat ccagggctga
      721 gtgagaggga agaccacaca ccctgtgagc ccactgtgtt ccagtgagtg ctgcactggg
      781 gtccacagca cactccaggg atcctgtgtg acacacctgt accttgtccc ccagagtcag
      841 gggctgggag tcattttctc tggctacaca cttagtgatg gctgttcact tggactgaca
      901 gttaatgttg gtcagcaagg tgactacaat ggttgagtct caatggtgtc accttccagg
      961 atcatacagc cctaatttta atatgaactc aaacacatat taaattagtt attttccatt
     1021 ccctcctcca ttctttgact acctctctca tgctattgaa catcacataa ggatggccat
     1081 gtttacccaa tggctcatgt ggattccctc ttagcttctg agtcccaaaa gaaaatgtgc
     1141 agtcctgtgc tgaggggacc agctctgctt ttggtcacta gtgcgatgac agttgaagtg
     1201 tcaaacagac acatagttca ctgtcatcat tgatttaact gagtcttggg tagatttcag
     1261 tttgtcttgt taattgtgtg atttcttaaa tcttccacac agattcccca aaggcccatg
     1321 tgacccatca cagcagacct gaagataaag tcaccctgag gtgctgggcc ctgggcttct
     1381 accctgctga catcaccctg acctggcagt tgaatgggga ggagctgatc caggacatga
     1441 agcttgtgga gaccaggcct gcaggggatg gaaccttcca gaagtgggca tctgtggtgg
     1501 tgcctcttgg gaaggagcag tattacacat gccatgtgta ccatcagggg ctgcctgagc
     1561 ccctcaccct gagatggggt aaggagagtg tgggtgcaga gctggggtca gggaaagctg
     1621 gagctttctg cagaccctga gctgctcagg gctgagagct ggggtcatga ccctcacctt
     1681 catttcttgt acctgtcctt cccagagcct cctccatcca ctgtctccaa catggcgacc
     1741 gttgctgttc tggttgtcct tggagctgca atagtcactg gagctgtggt ggcttttgtg
     1801 atgaagatga gaaggagaaa cacaggtagg aaagggcaga gtctgagttt tctctcagcc
     1861 tcctttagag tgtgctctgc tcatcaatgg ggaacacagg cacaccccac attgctactg
     1921 tctctaactg ggtctgctgt cagttctggg aacttcctag tgtcaagatc ttcctggaac
     1981 tctcacagct tttcttctca caggtggaaa aggaggggac tatgctctgg ctccaggtta
     2041 gtgtggggac agagttgtcc tggggacatt ggagtgaagt tggagatgat gggagctctg
     2101 ggaatccata atagctcctc cagagaaatc ttctaggtgc ctgagttgtg ccatgaaatg
     2161 aatatgtaca tgtacatatg catatacatt tgttttgtta taccctaggc tcccagacct
     2221 ctgatctgtc tctcccagat tgtaaaggtg acactctagg gtctgattgg ggaggggcaa
     2281 tgtggacatg attgggtttc aggaactccc agaatcccct gtgagcgagt gatgggttgt
     2341 tcgaatgttg tcttcacagt gatggttcat gaccctcatt ctctagcgtg aagacagctg
     2401 cctggagtgg acttggtgac agacaatgtc ttctcatatc tcctgtgaca tccagagccc
     2461 tcagttctct ttagtcaagt gtctgatgtt ccctgtgagc ctatggactc aatgtgaaga
     2521 actgtggagc ccagtccacc cctctacacc aggaccctgt ccctgcactg ctctgtcttc
     2581 ccttccacag ccaaccttgc tggttcagcc aaacactgag ggacatctgt agcctgtcag
     2641 ctccatgcta ccctgacctg caactcctca cttccacact gagaataata atttgaatgt
     2701 aaccttgatt gttatcatca cgacctaggg ctgatttctt gttaatttca tggattgaga
     2761 atgcttagag gttttgtttg tttgtttggt tgatttgttt ttttgaagaa ataaatgata
     2821 gatgaataaa cttccagaat ctgggtcact aataagccag ccccgacacc cgccaacacc
     2881 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     2941 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     3001 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     3061 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3121 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3181 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3241 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3301 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3361 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3421 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3481 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3541 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3601 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3661 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3721 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3781 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3841 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     3901 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     3961 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4021 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4081 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4141 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4201 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4261 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4321 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4381 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4441 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4501 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4561 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4621 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4681 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4741 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4801 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4861 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     4921 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     4981 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     5041 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     5101 aattaatgtg gaaccaatca gtgtcgccgc ggacgctgga tataaagtcc acgcagcccg
     5161 cagaactcag aagtcgcgaa tcgccgacag gtgcgatggt accgtgcacg ctgctcctgc
     5221 tgttggcggc cgccctggct ccgactcaga cccgcgcggg tgagtaccgg gccgggaggg
     5281 aaacggcctc tgaggggagg ggcggcaccg gggaagccgc gtcctcgggt cgcccaccgg
     5341 accctccgcc ccttctccac ccgagtcccg cgccctgctc ccctctcagc ccgcgcagcc
     5401 gcccggggtc tggtgaggtg gtccgggtct caccgcgcgc cgcccccagg cccacactcg
     5461 ctgaggtatt tcgtcaccgc cgtgtcccgg cccggcctcg gggagccccg gtacatggaa
     5521 gtcggctacg tggacgacac ggagttcgtg cgcttcgaca gcgacgcgga gaatccgaga
     5581 tatgagccgc gggcgcggtg gatggagcag gaggggcccg agtattggga gcgggagaca
     5641 cagaaagcca agggcaatga gcagagtttc cgagtggacc tgaggaccct gctcggctac
     5701 tacaaccaga gcaagggcgg tgagtgaccc cgggtcggag gtcacgaccc ctccacgtcc
     5761 cgacacaggg acgctgacgt cccgggtccc aagtccgagg ttcgggaaca gaacggaccc
     5821 ggaaccggtt tctctttcag tttggaggag tccgcgggcg ggcggggccg gggcgggtga
     5881 gcggggctga ccgcggggtc ccgcaggctc tcacactatt caggtgatct ctggctgtga
     5941 agtggggtcc gacgggcgac tcctccgcgg gtaccagcag tacgcctacg acggctgcga
     6001 ttacatcgcc ctgaacgaag acctgaaaac gtggacggcg gcgggcatgg cggcgctgat
     6061 caccaaacac aagtgggagc aggctggtga agcagagaga ctcagggcct acctggaggg
     6121 cacgtgcgtg gagtggctcc gcagatacct gaaggacggg aacgcgacgc tgctgcgcac
     6181 aggtgcaggg gccgcgggca gctcctccct ctgccctcgg gctggggctc agtcctgggg
     6241 aagaagaaac cctcagctgg ggtgatgccc ctgtctcaga ggggagagag tgtcgctggt
     6301 ctcctgatcc ctcatcacag tgactgcact gactctccca gggctcagcc ttctccctgg
     6361 acagtgccca ggctgtctca ggagggaagg agagaatttc cctgaggtaa caacagctgc
     6421 tcccttcagt tcccctgcag cctctgtcag ccatggcctc tcccaggccg ggttctctgc
     6481 ccacgcccac tgtctgtaga cactgactcc tgtcctgctg aatgtgtcag cccttacacc
     6541 tcaggaccgg aagtctcctt acctgataag agacatggac a
//
