LOCUS       sequence-201268-        8668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module 3 - GAL4 synthetic promoter (with eight binding sites) drives
            the expression of CCL21 transcripts containing miR1-Bs(Pe).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 8668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN491 (FuGW-G8p-CCL21-1Pe)
FEATURES             Location/Qualifiers
     source          1..8668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    241..358
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      694..703
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1161..1749
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1214..1234)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1632..1643)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1752..1768)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1753..1769)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2274..2454
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2480..2497)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2486..2710
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2756..3184
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2843..2862)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3053..3074
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3193..3213)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3198..3527
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3378..3513
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3440..3459
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3575..3622
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3641..4015
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4145..4266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4182..4201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4236..4255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4315..4331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4315..4331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4328..4350)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4339..4355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4363..4369,4370..4387,4388..4393))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4408..4429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4546..4563)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4717..5305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4797..4816)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5476..6267,6268..6336))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6099..6118
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6337..6441)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6516..6535)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6707..7086
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7088..7286
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7241..7261
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7304..7484
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7531..7656
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8149..8382
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8567..8611
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
       61 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
      121 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
      181 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
      241 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
      301 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      361 ggtttattac agggacagca gagatccagt ttggttaatc ctgcaggcga taggtaccga
      421 gtttctagac ggagtactgt cctccgagcg gagtactgtc ctccgactcg agcggagtac
      481 tgtcctccga tcggagtact gtcctccgcg aattccggag tactgtcctc cgaagacgct
      541 agacggagta ctgtcctccg agcggagtac tgtcctccga ctcgagcgga gtactgtcct
      601 ccggctagcg gggggctata aaagggggtg ggggcgttcg tcctcactct agatctgcga
      661 tctaagtaag cttggcattc cggtactggg cgcgccacca tggctcaatc cttggcgctg
      721 tccttgctga tcttggtgct ggcatttggc attccccgca cacaaggaag cgatggtggc
      781 gcacaggact gttgcctgaa gtattcacag cggaaaatcc cagctaaagt ggttcggagc
      841 tatcgcaagc aggaaccttc cttggggtgt tcaatcccag ctatcctctt tcttccaaga
      901 aagcgctctc aggccgagct gtgtgctgac ccaaaagagc tgtgggtcca gcagttgatg
      961 cagcacttgg acaagacgcc gtctccccag aaacccgccc agggctgcag aaaagatagg
     1021 ggcgcctcaa aaacaggcaa aaaagggaaa ggtagcaaag gttgcaaacg caccgaacga
     1081 agccagacgc caaaagggcc ataaccggtg caccgcttga agtctttaat taaataccgg
     1141 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     1201 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     1261 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     1321 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     1381 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     1441 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     1501 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     1561 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     1621 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     1681 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     1741 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     1801 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     1861 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     1921 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     1981 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     2041 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     2101 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     2161 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     2221 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     2281 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     2341 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     2401 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     2461 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     2521 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     2581 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     2641 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     2701 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     2761 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2821 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2881 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2941 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3001 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3061 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3121 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3181 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     3241 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3301 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3361 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3421 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     3481 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     3541 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     3601 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     3661 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     3721 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     3781 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     3841 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     3901 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     3961 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     4021 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     4081 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     4141 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4201 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4261 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     4321 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     4381 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     4441 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     4501 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     4561 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4621 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4681 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4741 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4801 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4861 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4921 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4981 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5041 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5101 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5161 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5221 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5281 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5341 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5401 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     5461 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     5521 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     5581 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     5641 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     5701 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     5761 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     5821 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5881 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5941 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6001 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6061 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     6121 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6181 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6241 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6301 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6361 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     6421 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     6481 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     6541 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     6601 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     6661 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     6721 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     6781 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     6841 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     6901 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     6961 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     7021 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     7081 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     7141 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     7201 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     7261 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
     7321 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7381 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7441 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     7501 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     7561 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     7621 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     7681 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     7741 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     7801 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     7861 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     7921 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     7981 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     8041 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     8101 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     8161 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     8221 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     8281 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     8341 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     8401 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     8461 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     8521 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     8581 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     8641 gtggtatata aaattattca taatgata
//
