LOCUS       sequence-201274-        9556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module 2 - synthetic promoter MAFK drives sponge 7 ,""optimized 
            sponge"" expression. (see PMID: 29056342 for detailed information).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9556)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 9556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN553 (FuGW-S(MAFK)p-Sponge7)
FEATURES             Location/Qualifiers
     source          1..9556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..391
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        393..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     546..566
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             609..789
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    836..961
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1454..1687
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1872..1916
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2214..2331
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      2653..2662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2704..2725)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2965..2984)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             3577..3828
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     3765..3786
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4022..4610
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4075..4095)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4493..4504)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4613..4629)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4614..4630)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5135..5315
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5341..5358)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5347..5571
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5617..6045
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5704..5723)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5914..5935
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6054..6074)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6059..6388
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6239..6374
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6301..6320
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6436..6483
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6502..6876
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7006..7127
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7043..7062)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7097..7116
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7176..7192)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7176..7192)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7189..7211)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7200..7216
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7224..7230,7231..7248,7249..7254))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7269..7290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7407..7424)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7578..8166)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7658..7677)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8337..9128,9129..9197))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8960..8979
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9198..9302)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9377..9396)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
       61 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      121 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      181 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      241 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      301 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      361 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      421 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      481 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      541 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      601 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      661 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      721 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      781 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
      841 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
      901 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
      961 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1021 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1081 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1141 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1201 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1261 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1321 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1381 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1441 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1501 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1561 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1621 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1681 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1741 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1801 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1861 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1921 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1981 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2041 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2101 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2161 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2221 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2281 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2341 tacagggaca gcagagatcc agtttggtta atggtaccct gcactggaca cccttggaag
     2401 caaatcccct gcaggtgctg agtcagcaag atgctgagtc agcatcgtgc tgagtcagca
     2461 gactgctgag tcagcactat gctgagtcag caacttgctg agtcagcatg ctgctgagtc
     2521 agcagtatgc tgagtcagca ggcgcgccag acgctagcgg ggggctataa aagggggtgg
     2581 gggcgttcgt cctcactcta gatctgcgat ctaagtaagc ttggcattcc ggtactgttg
     2641 gtaaaccagc tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2701 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     2761 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2821 cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcgcccgc
     2881 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2941 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgagcttcct
     3001 gcctgacaca gctcacttca agaagtgcac atgtcagaac gtggaattaa gtgcatggac
     3061 gagctgtaca agtaaaggat ccgcttgaag gaataattaa agctagccgc ttgaaggaat
     3121 aattaaagat agccgcttga aggaataatt aaagctagcc gcttgaagga ataattaaag
     3181 ctagccgctt gaaggaataa ttaaagcttg taagcgggac tctggggttg aattcggatc
     3241 cgcttgaagg aataattaaa gctagccgct tgaaggaata attaaagata gccgcttgaa
     3301 ggaataatta aagctagccg cttgaaggaa taattaaagc tagccgcttg aaggaataat
     3361 taaagcttgt aagcgggact ctggggttaa agcaaattaa aaacaggatg aaaaagactg
     3421 attggctctt cctcaatgct tgtgtgggtg aagttcgagg gcgacaccct ggtgaaccgc
     3481 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     3541 tacaacgcca tcagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
     3601 gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     3661 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     3721 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3781 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aggatccgct
     3841 tgaaggaata attaaagcta gccgcttgaa ggaataatta aagatagccg cttgaaggaa
     3901 taattaaagc tagccgcttg aaggaataat taaagctagc cgcttgaagg aataattaaa
     3961 gcttgtaagc gggactctag ggttcgaaat gaccgaccaa gccgatatca agcttatcga
     4021 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     4081 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     4141 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     4201 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     4261 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     4321 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     4381 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     4441 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     4501 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     4561 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     4621 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     4681 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     4741 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4801 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     4861 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     4921 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     4981 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     5041 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     5101 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     5161 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5221 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5281 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     5341 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     5401 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     5461 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5521 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     5581 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     5641 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5701 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5761 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5821 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5881 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5941 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6001 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     6061 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6121 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6181 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6241 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6301 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     6361 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     6421 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     6481 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     6541 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     6601 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     6661 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     6721 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     6781 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     6841 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     6901 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6961 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     7021 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7081 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     7141 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7201 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7261 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7321 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7381 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7441 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7501 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7561 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7621 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7681 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7741 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7801 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7861 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7921 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7981 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8041 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8101 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8161 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8221 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8281 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8341 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8401 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8461 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8521 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8581 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8641 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8701 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8761 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8821 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8881 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8941 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9001 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9061 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9121 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9181 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9241 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9301 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     9361 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     9421 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     9481 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     9541 gcgatgtacg ggccag
//
