LOCUS       sequence-201278-        8922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic promoter USF1 drives mKate2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN592 (FuGW-S(USF1)p-mKate2)
FEATURES             Location/Qualifiers
     source          1..8922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..118
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     103..119
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    122..710
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             228..239
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     637..657
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      948..957
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             954..1667
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    1767..1884
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2182..2226)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2411..2644
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3137..3262
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3309..3489
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3507..3705)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3532..3552)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3707..4086
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4258..4277
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4352..4456
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4457..4525,4526..5317)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4675..4694)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5488..6076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5977..5996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6230..6247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6364..6385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6400..6405,6406..6423,6424..6430)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6438..6454
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6443..6465
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6462..6478
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6462..6478
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6527..6648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6538..6557)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6592..6611
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6778..7152)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7171..7218)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7266..7595)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7280..7415
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7334..7353)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7580..7600
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7609..8037)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7719..7740)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7931..7950
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8083..8307
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8296..8313
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8339..8519
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaaaaccca cctcctcctc ctcttgtgct tctagccagg cacaatcagc attggtagct
       61 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgaggtcg acggtatcga
      121 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
      181 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
      241 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
      301 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
      361 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
      421 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
      481 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
      541 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
      601 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
      661 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
      721 ttgatatcgc ggccgccctg cacctgcagg ccacgtgcag accacgtgct cgccacgtgc
      781 gacccacgtg cctaccacgt gcactccacg tgctgcccac gtgcgtacca cgtgcggcgc
      841 gccagacgct agcggggggc tataaaaggg ggtgggggcg ttcgtcctca ctctagatct
      901 gcgatctaag taagcttggc attccggtac tgttggtaaa ccagctcgcc accatggtgt
      961 ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     1021 tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     1081 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     1141 ctaccagctt catgtacggc agcaaaacct tcatcaacca cacccagggc atccccgact
     1201 tctttaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     1261 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     1321 tcaagatcag aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg
     1381 gctgggaggc ctccaccgag atgctgtacc ccgctgacgg cggcctggaa ggcagaagcg
     1441 acatggccct gaagctcgtg ggcgggggcc acctgatctg caacttgaag accacataca
     1501 gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactatgtg gacagaagac
     1561 tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag gtggctgtgg
     1621 ccagatactg cgacctccct agcaaactgg ggcacaaact taattgataa accggttgca
     1681 gtcccgggta agcgggactc tggggttcga aatgaccgac caagcattaa ccaaactgga
     1741 tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat
     1801 tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc
     1861 ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtct gcagaattgg
     1921 cgcacgcagt gccgatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc
     1981 ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa
     2041 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     2101 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     2161 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     2221 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     2281 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     2341 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     2401 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
     2461 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     2521 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     2581 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     2641 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     2701 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     2761 cctcatatct cctcctccag gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac
     2821 ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc
     2881 tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc
     2941 ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta
     3001 acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta
     3061 atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc
     3121 tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca
     3181 aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa
     3241 gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg
     3301 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     3361 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     3421 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     3481 gagagaccca gtacaggcaa aacgcgctgc ttatatagac ctcccaccgt acacgcctac
     3541 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     3601 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     3661 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     3721 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     3781 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     3841 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     3901 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     3961 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     4021 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc
     4081 aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa
     4141 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
     4201 cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg
     4261 catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc
     4321 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4381 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4441 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4501 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4561 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4621 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4681 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4741 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4801 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4861 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4921 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4981 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5041 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5101 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5161 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5221 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5281 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5341 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5401 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5461 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5521 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5581 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     5641 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5701 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5761 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5821 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5881 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5941 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6001 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6061 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6121 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6181 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6241 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6301 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     6361 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     6421 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     6481 tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag atacattgat
     6541 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     6601 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg ggtgggcgaa
     6661 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
     6721 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgta gcacgtgtca
     6781 gtcctgctcc tcggccacga agtgcacgca gttgccggcc gggtcgcgca gggcgaactc
     6841 ccgcccccac ggctgctcgc cgatctcggt catggccggc ccggaggcgt cccggaagtt
     6901 cgtggacacg acctccgacc actcggcgta cagctcgtcc aggccgcgca cccacaccca
     6961 ggccagggtg ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc
     7021 gtcccggacc acaccggcga agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc
     7081 ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt
     7141 caacttggcc atggtttagt tcctcacctt gtcgtattat actatgccga tatactatgc
     7201 cgatgattaa ttgtcaacac gtgctgatca gatccgaaaa tggatataca agctcccggg
     7261 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     7321 gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     7381 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     7441 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     7501 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     7561 gagcctgggg actttccaca ccctaactga cacacattcc acagaattaa ttcgcgttaa
     7621 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     7681 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     7741 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     7801 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     7861 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     7921 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     7981 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtggg
     8041 gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag cccaccgcat
     8101 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
     8161 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
     8221 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
     8281 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa acgggccctg
     8341 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     8401 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     8461 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     8521 gtacagtccg gatgcagctc tcgggccatg tgatgaaatg ctaggcggct gtcaaacctc
     8581 cactctaata cttctctctc cgggtcatcc atcccatgca ggctcacagg gtgtaacaag
     8641 cgggtgttct ctccttcatt ggcttcttct accttctctt gctcaactgg tactagcttg
     8701 tagcaccatc caaaggtcag tggatatctg atccctggcc ctggtgtgta gttctgccaa
     8761 tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg tcttcgttgg
     8821 gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct
     8881 gccttgtaag tcattggtct taaaggtacc tgaggtgtga ct
//
