LOCUS       sequence-201282-        8962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic promoter MAFK drives mKate2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 8962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN593 (FuGW-S(MAFK)p-mKate2)
FEATURES             Location/Qualifiers
     source          1..8962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..118
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     103..119
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    122..710
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             228..239
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     637..657
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      988..997
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             994..1707
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    1807..1924
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2222..2266)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2451..2684
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3177..3302
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3349..3529
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3547..3745)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3572..3592)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3747..4126
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4298..4317
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4392..4496
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4497..4565,4566..5357)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4715..4734)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5528..6116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6017..6036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6270..6287
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6404..6425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6440..6445,6446..6463,6464..6470)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6478..6494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6483..6505
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6502..6518
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6502..6518
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6567..6688
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6578..6597)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6632..6651
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6818..7192)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7211..7258)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7306..7635)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7320..7455
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7374..7393)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7620..7640
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7649..8077)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7759..7780)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7971..7990
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8123..8347
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8336..8353
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8379..8559
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaaaaccca cctcctcctc ctcttgtgct tctagccagg cacaatcagc attggtagct
       61 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgaggtcg acggtatcga
      121 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
      181 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
      241 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
      301 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
      361 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
      421 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
      481 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
      541 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
      601 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
      661 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
      721 ttgatatcgc ggccgccctg cacctgcagg tgctgagtca gcaagatgct gagtcagcat
      781 cgtgctgagt cagcagactg ctgagtcagc actatgctga gtcagcaact tgctgagtca
      841 gcatgctgct gagtcagcag tatgctgagt cagcaggcgc gccagacgct agcggggggc
      901 tataaaaggg ggtgggggcg ttcgtcctca ctctagatct gcgatctaag taagcttggc
      961 attccggtac tgttggtaaa ccagctcgcc accatggtgt ctaagggcga agagctgatt
     1021 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag
     1081 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
     1141 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
     1201 agcaaaacct tcatcaacca cacccagggc atccccgact tctttaagca gtccttccct
     1261 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
     1321 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
     1381 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag
     1441 atgctgtacc ccgctgacgg cggcctggaa ggcagaagcg acatggccct gaagctcgtg
     1501 ggcgggggcc acctgatctg caacttgaag accacataca gatccaagaa acccgctaag
     1561 aacctcaaga tgcccggcgt ctactatgtg gacagaagac tggaaagaat caaggaggcc
     1621 gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
     1681 agcaaactgg ggcacaaact taattgataa accggttgca gtcccgggta agcgggactc
     1741 tggggttcga aatgaccgac caagcattaa ccaaactgga tctctgctgt ccctgtaata
     1801 aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt
     1861 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
     1921 aaaattgtgg atgaatactg ccatttgtct gcagaattgg cgcacgcagt gccgatccgt
     1981 tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg
     2041 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct
     2101 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc
     2161 ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc
     2221 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggtttt
     2281 gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact
     2341 ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc
     2401 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt
     2461 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga
     2521 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct
     2581 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca
     2641 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt
     2701 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt
     2761 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
     2821 gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct
     2881 atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg
     2941 gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt
     3001 agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg
     3061 aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt tttctttccc
     3121 cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg
     3181 ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc
     3241 agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag
     3301 agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt
     3361 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
     3421 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
     3481 gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa
     3541 aacgcgctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
     3601 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
     3661 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
     3721 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
     3781 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
     3841 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
     3901 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
     3961 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
     4021 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
     4081 gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg
     4141 gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa gcagattctt catgcaattg
     4201 tcggtcaagc cttgccttgt tgtagcttaa attttgctcg cgcactactc agcgacctcc
     4261 aacacacaag cagggagcag atactggctt aactatgcgg catcagagca gattgtactg
     4321 agagtgcacc ataggggatc gggagatctc ccgatccgtc gacgtcaggt ggcacttttc
     4381 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4441 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4501 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4561 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4621 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4681 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4741 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4801 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4861 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4921 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4981 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5041 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5101 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5161 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5221 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5281 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5341 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5401 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5461 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5641 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6241 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6301 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6361 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6421 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6481 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc
     6541 tagaggtcga cggtatacag acatgataag atacattgat gagtttggac aaaccacaac
     6601 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     6661 aaccattata agctgcaata aacaagttgg ggtgggcgaa gaactccagc atgagatccc
     6721 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
     6781 agaaggcggc ggtggaatcg aaatctcgta gcacgtgtca gtcctgctcc tcggccacga
     6841 agtgcacgca gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc
     6901 cgatctcggt catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc
     6961 actcggcgta cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca
     7021 ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga
     7081 agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc
     7141 cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt caacttggcc atggtttagt
     7201 tcctcacctt gtcgtattat actatgccga tatactatgc cgatgattaa ttgtcaacac
     7261 gtgctgatca gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag
     7321 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc
     7381 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     7441 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     7501 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     7561 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     7621 ccctaactga cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca
     7681 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     7741 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     7801 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     7861 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     7921 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     7981 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     8041 acacccgccg cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag
     8101 ctggttcttt ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg
     8161 tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac
     8221 ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca
     8281 ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa
     8341 ggcacagtcg aggctgatca gcgggtttaa acgggccctg ctagagattt tccacactga
     8401 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     8461 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     8521 gagctcccag gctcagatct ggtctaacca gagagaccca gtacagtccg gatgcagctc
     8581 tcgggccatg tgatgaaatg ctaggcggct gtcaaacctc cactctaata cttctctctc
     8641 cgggtcatcc atcccatgca ggctcacagg gtgtaacaag cgggtgttct ctccttcatt
     8701 ggcttcttct accttctctt gctcaactgg tactagcttg tagcaccatc caaaggtcag
     8761 tggatatctg atccctggcc ctggtgtgta gttctgccaa tcagggaagt agccttgtgt
     8821 gtggtagatc cacagatcaa ggatatcttg tcttcgttgg gagtgaatta gcccttccag
     8881 tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct
     8941 taaaggtacc tgaggtgtga ct
//
