LOCUS       sequence-201285-       11093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ubiquitin C promoter drives STE expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 11093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN599 (pFuGW-hUbCp-hSTE1)
FEATURES             Location/Qualifiers
     source          1..11093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    241..358
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        423..1634
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1471..1490
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1759..1785
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2521..2541
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     misc_feature    3586..4174
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3639..3659)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4057..4068)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4177..4193)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4178..4194)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4699..4879
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4905..4922)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4911..5135
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5181..5609
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5268..5287)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5478..5499
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5618..5638)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5623..5952
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5803..5938
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5865..5884
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6000..6047
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6066..6440
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6570..6691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6607..6626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6661..6680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6740..6756)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6740..6756)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6753..6775)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6764..6780
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6788..6794,6795..6812,6813..6818))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6833..6854
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6971..6988)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7142..7730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7222..7241)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7901..8692,8693..8761))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8524..8543
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8762..8866)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8941..8960)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9132..9511
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9513..9711
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9666..9686
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9729..9909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9956..10081
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10574..10807
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10992..11036
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
       61 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
      121 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
      181 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
      241 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
      301 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      361 ggtttattac agggacagca gagatccagt ttggttaatt aacccgtgtc ggctccagat
      421 ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc
      481 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac
      541 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
      601 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
      661 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
      721 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
      781 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg
      841 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
      901 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
      961 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt
     1021 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc
     1081 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
     1141 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
     1201 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
     1261 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
     1321 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
     1381 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
     1441 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     1501 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     1561 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     1621 cttttttgtt agacagtgcg atctaagtaa gcttggcatt ccggtactgt tggtaaagcc
     1681 gccaccatgc ggctctgcat cccgcaggtg ctgttggcct tgttcctttc catgctgaca
     1741 gggccgggag aaggcagcta cccttacgat gtacctgatt acgccggcgg cggaggctca
     1801 caagtgcagc ttcagcagag cggcgctgag ctggcccggc ccggggcctc tgtgaaaatg
     1861 agctgcaagg cttccggtta cacctttaca aggtacacaa tgcactgggt gaagcagcgg
     1921 ccagggcagg ggctcgagtg gatcggctac ataaacccat ctcggggata tacaaactac
     1981 aatcaaaagt tcaaagacaa ggccacactg acaacggaca agtcaagcag cacagcttac
     2041 atgcaactga gctctctgac atccgaggat agcgccgttt actattgcgc cagatactat
     2101 gatgaccact attgcttgga ctactgggga cagggaacaa ccctcacagt tagctccggc
     2161 ggggggggca gtggaggtgg aggatctggg ggcggcggta gtcagatcgt cctcacacag
     2221 tctccggcca taatgtccgc ctcccccgga gagaaggtta ctatgacatg ttccgcatct
     2281 tcctctgtgt catatatgaa ttggtaccag cagaagagtg gcacctctcc taaacgctgg
     2341 atttacgata cctctaaact ggcgtccggg gtgcctgcac atttccgcgg atccggctcc
     2401 ggtacgagtt attcactcac aatatctgga atggaggccg aagatgccgc tacttactac
     2461 tgccaacaat ggtcaagcaa ccccttcact ttcgggagcg ggacaaagct ggagatcaac
     2521 caccatcatc atcaccatca tgcaggtatg gcctcctctg ggtatgtcct ccaggcggag
     2581 ctctccccct caactgagaa ctcaagtcag ctggacttcg aagatgtatg gaattcttcc
     2641 tatggtgtga atgattcctt cccagatgga gactatgatg ccaacctgga agcagctgcc
     2701 ccctgccact cctgtaacct gctggatgac tctgcactgc ccttcttcat cctcaccagt
     2761 gtcctgggta tcctagctag cagcactgtc ctcttcatgc ttttcagacc tctcttccgc
     2821 tggcagctct gccctggctg gcctgtcctg gcacagctgg ctgtgggcag tgccctcttc
     2881 agcattgtgg tgcccgtctt ggccccaggg ctaggtagca ctcgcagctc tgccctgtgt
     2941 agcctgggct actgtgtctg gtatggctca gcctttgccc aggctttgct gctagggtgc
     3001 catgcctccc tgggccacag actgggtgca ggccaggtcc caggcctcac cctggggctc
     3061 actgtgggaa tttggggagt ggctgcccta ctgacactgc ctgtcaccct ggccagtggt
     3121 gcttctggtg gactctgcac cctgatatac agcacggagc tgaaggcttt gcaggccaca
     3181 cacactgtag cctgtcttgc catctttgtc ttgttgccat tgggtttgtt tggagccaag
     3241 gggctgaaga aggcattggg tatggggcca ggcccctgga tgaatatcct gtgggcctgg
     3301 tttattttct ggtggcctca tggggtggtt ctaggactgg atttcctggt gaggtccaag
     3361 ctgttgctgt tgtcaacatg tctggcccag caggctctgg acctgctgct gaacctggca
     3421 gaagccctgg caattttgca ctgtgtggct acgcccctgc tcctcgccct attctgccac
     3481 caggccaccc gcaccctctt gccctctctg cccctccctg aaggatggtc ttctcatctg
     3541 gacacccttg gaagcaaatc ctaacccgat atcaagctta tcgataatca acctctggat
     3601 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3661 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3721 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3781 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3841 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3901 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3961 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     4021 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4081 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4141 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta
     4201 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     4261 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     4321 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     4381 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     4441 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
     4501 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
     4561 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     4621 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
     4681 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     4741 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     4801 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     4861 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     4921 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     4981 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     5041 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     5101 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     5161 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5221 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5281 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     5341 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     5401 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5461 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5521 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5581 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     5641 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5701 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5761 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     5821 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5881 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5941 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     6001 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     6061 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
     6121 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
     6181 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
     6241 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
     6301 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
     6361 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
     6421 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
     6481 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     6541 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     6601 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6661 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     6721 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6781 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6841 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6901 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6961 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7141 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7321 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7561 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7741 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7801 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7861 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7921 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7981 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8041 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8101 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8161 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8221 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8281 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8341 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8401 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8461 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8521 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8581 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8641 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8701 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8761 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8821 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8881 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     8941 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     9001 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     9061 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     9121 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     9181 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     9241 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     9301 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     9361 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     9421 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     9481 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     9541 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     9601 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     9661 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
     9721 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9781 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9841 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9901 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     9961 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
    10021 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
    10081 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
    10141 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
    10201 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
    10261 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
    10321 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
    10381 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
    10441 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
    10501 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
    10561 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
    10621 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
    10681 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
    10741 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
    10801 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
    10861 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
    10921 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
    10981 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
    11041 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg ata
//
