LOCUS       sequence-201293-        5239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression 
            (Version 3 intron - see PMID: 29056342 for detailed information).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3
            intron]-[mK-E...
FEATURES             Location/Qualifiers
     source          1..5239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(108..127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(787..1578,1579..1647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1410..1429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1648..1752)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1779..2234
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1866..1885)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2076..2097
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     regulatory      3209..3218
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     ncRNA           3675..3689
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3746..3858
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(4689..4708)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4733..4780
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(5096..5113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
       61 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
      721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
      781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
      841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
      901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
      961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1081 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1381 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     1681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     1741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
     1801 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1861 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1921 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1981 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     2041 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2101 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2161 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     2221 aacgcttaca atttgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     2281 gcgggcctct tcgctattac gccagcccaa gctaccatga taagtaagta atattaaggt
     2341 acgggaggta cttggaggta cccggccgcc ctgcagggta gccagatcat ggctcactgc
     2401 aacctcgtac tcctgggctc aagctatcct cctacctcag cctcctgagt aacggactac
     2461 aggcacacca ccccacctcg ctaattttat ttattttttt tgtagagaaa agagacaggg
     2521 tattgctctg ttgcccaggg tggggtgcag tggcatgatc atggctcact gcaacctctg
     2581 cctcccaggt tcaagtgatc ctccagctgt ggcctcccta agtgctggga ttacaaccgt
     2641 gagccgccgc accggcccaa atttcttacg tcactacaga gttcctagga aaaaatccca
     2701 tacctgaaaa agatagaaac tgacaggaag gatttgagat gatgacctgc ttcatataca
     2761 ctccttatta aaactggata acaatgcacc accgaggagg tggggaggga taggaaaaat
     2821 gaaaagagaa aatcagcgca tgcgtactct gatttgggaa gactccaaag agaaaatcag
     2881 agcatgcgta ctctgaactt ggagtagcca atcccagggg atgctttagg cgggaaagtc
     2941 agagtttctg cctccatttt gagaaggttc tgtccctaga gcctagactg atagacccca
     3001 catcagcttg gcttgtcccg cctactgttc tgacttctga ttggccagat ggagttcact
     3061 aactgccctg attggtccat catcctggag caatgacatt gcagaatatt ttctcctcct
     3121 ccagccacac tttgtcacca actgctgcca actcgccacc actgctgccg acctcgcaac
     3181 cactgctttg tctctggcgc gccagctcgc caccatggtg tctaagggcg aagagctgat
     3241 taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
     3301 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
     3361 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
     3421 cagcaaaacc ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc
     3481 tgaggtaagt gtgctcgctt cggcagcaca tatactatgt tgaatgaggc ttcagtactt
     3541 tacagaatcg ttgcctgcac atcttggaaa cacttgctgg gattacttct tcagggactt
     3601 cttaacccaa cagaaggctc gagaaggtat attgctgttg acagtgagcg ccgcttgaag
     3661 tctttaatta aatagtgaag ccacagatgt atttaattaa agacttcaag cggtgcctac
     3721 tgcctcggac ttcaaggggc tagaattcaa ggggctactt taggagcaat tatcttgttt
     3781 actaaaactg aataccttgc tatctctttg atacattttt acaaagctga attaaaatgg
     3841 tataaattaa atcacttttt tcaattgtac taacttcgag tcttcttttt ttttttcaca
     3901 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
     3961 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
     4021 cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct ccaccgagat
     4081 gctgtacccc gctgacggcg gcctggaagg cagaagcgac atggccctga agctcgtggg
     4141 cgggggccac ctgatctgca acttgaagac cacatacaga tccaagaaac ccgctaagaa
     4201 cctcaagatg cccggcgtct actatgtgga cagaagactg gaaagaatca aggaggccga
     4261 caaagagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag
     4321 caaactgggg cacaaactta attgataaac ataggatggc aagatcctgg tatcggtctg
     4381 cgagcaccgc ttgaagtctt taattaaata cgcaccgctt gaagtcttta attaaatacg
     4441 caccgcttga agtctttaat taaatacctc gagcctctgg ccacatcggt tcctgcggta
     4501 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     4561 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     4621 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     4681 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     4741 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     4801 cagggctggc actctgtcga taccccaccg agacgccatt ggggccaata cgcccgcgtt
     4861 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     4921 gtcggggcgg caggccctgc catagcctca ggtatgtcga ccgatgccct tgagagcctt
     4981 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     5041 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc
     5101 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5221 gccagcaaaa ggccaggaa
//
