LOCUS       sequence-201300-       12334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancing the upper-part mevalonate pathway; overexpressing yeast 
            mevalonate pathway genes under the control of consitutive promoters.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12334)
  AUTHORS   Peng B, Plan MR, Chrysanthopoulos P, Hodson MP, Nielsen LK, Vickers 
            CE
  TITLE     A squalene synthase protein degradation method for improved 
            sesquiterpene production in Saccharomyces cerevisiae.
  JOURNAL   Metab Eng. 2017 Jan;39:209-219. doi: 10.1016/j.ymben.2016.12.003. 
            Epub 2016 Dec 8.
  PUBMED    27939849
REFERENCE   2  (bases 1 to 12334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2017-01"; volume: "39:209-219. doi"; pages: " 
            10.1016/j.ymben.2016.12.003. Epub 2016 Dec 8"
COMMENT     Sequence Label: pPMVAu5 (R)
FEATURES             Location/Qualifiers
     source          1..12334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(394..414)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(394..412)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(433..449)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(433..449)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(446..468)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    457..473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(481..487,488..505,506..511))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    526..547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(664..681)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(835..1423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(915..934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1594..2385,2386..2454))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2217..2236
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2455..2559)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2586..3928
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(3549..3596)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     3969..3987
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4025..4047)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4147..4166
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4313..4500
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             4501..5163
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     CDS             11697..11708
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cttcatatga atactaggta gaaatttggc ttatcagtac gaatgaatta cagtttgact
       61 gatttgtaca cgagaattga cgtataactt cttttgaaag tctttgaaat tacatggtag
      121 tacatacata tatatatata tatatatgtt atattattgt taattaaata ttattatgcg
      181 tattttcctt ttctttatta gtatagtatt aatgacagta ataataataa taatagtaac
      241 aatatctctt tttttttcag tgagctttta tttttttttc attgctcttc ttttggcctc
      301 ttttgttttt ttcttgattt cctccagttt catctgtttt ttctttggat cagatacaaa
      361 atctggtttg aacgcgagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
      421 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      481 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      541 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      601 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      661 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      721 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      781 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      841 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      901 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      961 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1021 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1081 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1141 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1201 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1261 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1321 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1381 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1441 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1501 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1561 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1621 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1681 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1741 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1801 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1861 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1921 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1981 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2041 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2101 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2161 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2221 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2281 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2341 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2401 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2461 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2521 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2581 acctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa
     2641 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc
     2701 tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc
     2761 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag
     2821 agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg
     2881 agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta
     2941 taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac
     3001 tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat
     3061 tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt atattctata
     3121 ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg
     3181 gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt
     3241 ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt
     3301 ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt
     3361 caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa
     3421 agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta
     3481 cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa
     3541 agcgctctga agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag
     3601 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
     3661 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
     3721 tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt
     3781 agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag
     3841 cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca
     3901 atgctatcat ttcctttgat attggatcat actaagaaac cattattatc atgacattaa
     3961 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     4021 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     4081 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     4141 actatgcggc atcagagcag attgtactga gagtgcacca taaattcccg ttttaagagc
     4201 ttggtgagcg ctaggagtca ctgccaggta tcgtttgaac acggcattag tcagggaagt
     4261 cataacacag tcctttcccg caattttctt tttctattac tcttggcctc ctctagtaca
     4321 ctctatattt ttttatgcct cggtaatgat tttcattttt ttttttccac ctagcggatg
     4381 actctttttt tttcttagcg attggcatta tcacataatg aattatacat tatataaagt
     4441 aatgtgattt cttcgaagaa tatactaaaa aatgagcagg caagataaac gaaggcaaag
     4501 atgacagagc agaaagccct agtaaagcgt attacaaatg aaaccaagat tcagattgcg
     4561 atctctttaa agggtggtcc cctagcgata gagcactcga tcttcccaga aaaagaggca
     4621 gaagcagtag cagaacaggc cacacaatcg caagtgatta acgtccacac aggtataggg
     4681 tttctggacc atatgataca tgctctggcc aagcattccg gctggtcgct aatcgttgag
     4741 tgcattggtg acttacacat agacgaccat cacaccactg aagactgcgg gattgctctc
     4801 ggtcaagctt ttaaagaggc cctaggggcc gtgcgtggag taaaaaggtt tggatcagga
     4861 tttgcgcctt tggatgaggc actttccaga gcggtggtag atctttcgaa caggccgtac
     4921 gcagttgtcg aacttggttt gcaaagggag aaagtaggag atctctcttg cgagatgatc
     4981 ccgcattttc ttgaaagctt tgcagaggct agcagaatta ccctccacgt tgattgtctg
     5041 cgaggcaaga atgatcatca ccgtagtgag agtgcgttca aggctcttgc ggttgccata
     5101 agagaagcca cctcgcccaa tggtaccaac gatgttccct ccaccaaagg tgttcttatg
     5161 tagtgacacc gattatttaa agctgcagca tacgatatat atacatgtgt atatatgtat
     5221 acctatgaat gtcagtaagt atgtatacga acagtatgat actgaagatg acaaggtaat
     5281 gcatcattct atacgtgtca ttctgaacga ggcgcgcttt ccttttttct ttttgctttt
     5341 tctttttttt tctcttgaac tcgacggatc tatgcggtgt gaaataccgc acagatgcgt
     5401 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta
     5461 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     5521 aaggatccat agtgggtaga taaccttaaa ctatttattt agaggcgaca atgtgaaaaa
     5581 ttttttcttc aaaggctcgg ttgtcgacaa attgttacgt tgtgctttga tttctaaagc
     5641 gcttcttcac ctgcaggttc tgagccctaa gaaaaaaaat ttccttggtt gaaaatggcg
     5701 gaaaaaaaaa attcagaaaa agaaataaag cacgtgtgcg cggtgtgtgg atgatggttt
     5761 catcattgtc aacggcattt tcgttcttgt ggattgttgt aaactttcca gaacattcta
     5821 gaaagaaagc acacggaacg tttagaagct gtcatttgcg ttttttctcc agattttagt
     5881 tgagaaagta attaaattat tcttcttttt ccagaacgtt ccatcggcgg caaaagggag
     5941 agaaagaacc caaaaagaag gggggccatt tagattagct gatcgtttcg aggacttcaa
     6001 ggttatataa ggggtggatt gatgtatctt cgagaaggga ttgagttgta gtttcgtttc
     6061 ccaattctta cttaagttgt tttattttct ctatttgtaa gataagcaca tcaaaagaaa
     6121 agtaatcaag tattacaaga aacaaaaatt caagtaaata acagataaaa tgtctcagaa
     6181 cgtttacatt gtatcgactg ccagaacccc aattggttca ttccagggtt ctctatcctc
     6241 caagacagca gtggaattgg gtgctgttgc tttaaaaggc gccttggcta aggttccaga
     6301 attggatgca tccaaggatt ttgacgaaat tatttttggt aacgttcttt ctgccaattt
     6361 gggccaagct ccggccagac aagttgcttt ggctgccggt ttgagtaatc atatcgttgc
     6421 aagcacagtt aacaaggtct gtgcatccgc tatgaaggca atcattttgg gtgctcaatc
     6481 catcaaatgt ggtaatgctg atgttgtcgt agctggtggt tgtgaatcta tgactaacgc
     6541 accatactac atgccagcag cccgtgcggg tgccaaattt ggccaaactg ttcttgttga
     6601 tggtgtcgaa agagatgggt tgaacgatgc gtacgatggt ctagccatgg gtgtacacgc
     6661 agaaaagtgt gcccgtgatt gggatattac tagagaacaa caagacaatt ttgccatcga
     6721 atcctaccaa aaatctcaaa aatctcaaaa ggaaggtaaa ttcgacaatg aaattgtacc
     6781 tgttaccatt aagggattta gaggtaagcc tgatactcaa gtcacgaagg acgaggaacc
     6841 tgctagatta cacgttgaaa aattgagatc tgcaaggact gttttccaaa aagaaaacgg
     6901 tactgttact gccgctaacg cttctccaat caacgatggt gctgcagccg tcatcttggt
     6961 ttccgaaaaa gttttgaagg aaaagaattt gaagcctttg gctattatca aaggttgggg
     7021 tgaggccgct catcaaccag ctgattttac atgggctcca tctcttgcag ttccaaaggc
     7081 tttgaaacat gctggcatcg aagacatcaa ttctgttgat tactttgaat tcaatgaagc
     7141 cttttcggtt gtcggtttgg tgaacactaa gattttgaag ctagacccat ctaaggttaa
     7201 tgtatatggt ggtgctgttg ctctaggtca cccattgggt tgttctggtg ctagagtggt
     7261 tgttacactg ctatccatct tacagcaaga aggaggtaag atcggtgttg ccgccatttg
     7321 taatggtggt ggtggtgctt cctctattgt cattgaaaag atataatgat aacctgcaac
     7381 tcctcaatgt gtcaattaac tcttacttaa tttatgtata tattttttat gtatatgctt
     7441 atatgcatgc gcatatgctc ataaaagata cattgttata ggtcatttct tttccaagct
     7501 acatctagca ctccttccaa ccatggtcca ggacagccag gaccttttct tgccaaaatt
     7561 cttgctttcc catctcagag aaaaaacgag gaatttttct ttgtcctctt tggccgccag
     7621 ttgcggtaaa atattttttt tctaggtcgc atagtttgaa agacttgtct ttactagtaa
     7681 atcagacaat ctgtctgacg atatagagag cgaagaggaa gaggaagagg gtgatgacag
     7741 cttggaatca tggctttccc agctttacat agactcaagt ggcgagcctt caccctcaac
     7801 gtgggcctta gctaaccttt tgactttaac ggccgttcaa tgggaatcat tgttctccaa
     7861 aaaggccact cctcacatca gtgattctat agttaacgaa gaaaagctgc ccttcctggc
     7921 caaaaaagac aatccccatt ccaagaaatt gctttccaaa ttacttaagg aaaagcaact
     7981 accttgcata aaaggcgaca actcgtcaaa gataacaagt gctactaaaa gcatgctcca
     8041 aaatgctcga acacttgcac aatcggagca taatatctta gatagatgtc tcaagagact
     8101 atcctaaata atattgaata tgcactttta ctatattaat atcacgtcac acgacgcaca
     8161 gtgagaagtg aaaaattttt tttcaatctg aaaaaaaaaa aaaaaanttt atataaacga
     8221 atggtatctc catcacattt cttttagcct cgcaacttat acttttcatc acttttcttg
     8281 taatttagca atatcccaag aacaatcatc aaaatgaaac tctcaactaa actttgttgg
     8341 tgtggtatta aaggaagact taggccgcaa aagcaacaac aattacacaa tacaaacttg
     8401 caaatgactg aactaaaaaa acaaaagacc gctgaacaaa aaaccagacc tcaaaatgtc
     8461 ggtattaaag gtatccaaat ttacatccca actcaatgtg tcaaccaatc tgagctagag
     8521 aaatttgatg gcgtttctca aggtaaatac acaattggtc tgggccaaac caacatgtct
     8581 tttgtcaatg acagagaaga tatctactcg atgtccctaa ctgttttgtc taagttgatc
     8641 aagagttaca acatcgacac caacaaaatt ggtagattag aagtcggtac tgaaactctg
     8701 attgacaagt ccaagtctgt caagtctgtc ttgatgcaat tgtttggtga aaacactgac
     8761 gtcgaaggta ttgacacgct taatgcctgt tacggtggta ccaacgcgtt gttcaactct
     8821 ttgaactgga ttgaatctaa cgcatgggat ggtagagacg ccattgtagt ttgcggtgat
     8881 attgccatct acgataaggg tgccgcaaga ccaaccggtg gtgccggtac tgttgctatg
     8941 tggatcggtc ctgatgctcc aattgtattt gactctgtaa gagcttctta catggaacac
     9001 gcctacgatt tttacaagcc agatttcacc agcgaatatc cttacgtcga tggtcatttt
     9061 tcattaactt gttacgtcaa ggctcttgat caagtttaca agagttattc caagaaggct
     9121 atttctaaag ggttggttag cgatcccgct ggttcggatg ctttgaacgt tttgaaatat
     9181 ttcgactaca acgttttcca tgttccaacc tgtaaattgg tcacaaaatc atacggtaga
     9241 ttactatata acgatttcag agccaatcct caattgttcc cagaagttga cgccgaatta
     9301 gctactcgcg attatgacga atctttaacc gataagaaca ttgaaaaaac ttttgttaat
     9361 gttgctaagc cattccacaa agagagagtt gcccaatctt tgattgttcc aacaaacaca
     9421 ggtaacatgt acaccgcatc tgtttatgcc gcctttgcat ctctattaaa ctatgttgga
     9481 tctgacgact tacaaggcaa gcgtgttggt ttattttctt acggttccgg tttagctgca
     9541 tctctatatt cttgcaaaat tgttggtgac gtccaacata ttatcaagga attagatatt
     9601 actaacaaat tagccaagag aatcaccgaa actccaaagg attacgaagc tgccatcgaa
     9661 ttgagagaaa atgcccattt gaagaagaac ttcaaacctc aaggttccat tgagcatttg
     9721 caaagtggtg tttactactt gaccaacatc gatgacaaat ttagaagatc ttacgatgtt
     9781 aaaaaataat gataactttg atctgtagcc taagtataaa attctacgta tgtatatatt
     9841 tacatgcaat tttttctttt tccaattcat gttaatgttc ttcatcattt gataataggc
     9901 caatgatatt atctactatc ttctcctttt gaggagaagc tgctttcact tcctcgttgc
     9961 ttttctcttc gtataaatac atgaatttcg acttgtccag cattttttta tcaatacaat
    10021 gcatgtgccc atgaagttct acctccgaca aatctttaac aaaatatgtc gttatttgtg
    10081 tcatgaattt cagtactcga ggtattcaag atgttcttat caaagcactt attttttcat
    10141 tatatttcta tattgattta gggacggtac gctattttct ttgtaccttt tactatgcat
    10201 aaaaaaaggg gaagtgttca acaatcgaca cttacttaat atgttttgcc gcccttcata
    10261 agagggtgtt tctaaaattt attgggcaag aatgagatgg actcgcaccc tacataacgt
    10321 ttagatattt agtgttaagt tcaaaacatg catcaggtgc gacatgtgtt gcgattatca
    10381 tgacaatgtc tctatccgag atgcatttgt agtatcaact gatgcatatt atgacatgat
    10441 tcacatagca tacatcgtca aacatgatgt tatttttttg ataaatgtac ggatttaaag
    10501 ctgtcgaata tattttctga aatttcttgg agccgacgca aaattttcaa aggtgctaaa
    10561 atttttcaag atttctcact tttgcttggt aacaaagaat gatggcattg catttttacc
    10621 accggtacat ttaactgcta tttctcacgt ttctttctct atccttaagt aattctttta
    10681 cagtctaaga aaaccacgat caaacaaata aatcaacaat ggaccaattg gtgaaaactg
    10741 aagtcaccaa gaagtctttt actgctcctg tacaaaaggc ttctacacca gttttaacca
    10801 ataaaacagt catttctgga tcgaaagtca aaagtttatc atctgcgcaa tcgagctcat
    10861 caggaccttc atcatctagt gaggaagatg attcccgcga tattgaaagc ttggataaga
    10921 aaatacgtcc tttagaagaa ttagaagcat tattaagtag tggaaataca aaacaattga
    10981 agaacaaaga ggtcgctgcc ttggttattc acggtaagtt acctttgtac gctttggaga
    11041 aaaaattagg tgatactacg agagcggttg cggtacgtag gaaggctctt tcaattttgg
    11101 cagaagctcc tgtattagca tctgatcgtt taccatataa aaattatgac tacgaccgcg
    11161 tatttggcgc ttgttgtgaa aatgttatag gttacatgcc tttgcccgtt ggtgttatag
    11221 gccccttggt tatcgatggt acatcttatc atataccaat ggcaactaca gagggttgtt
    11281 tggtagcttc tgccatgcgt ggctgtaagg caatcaatgc tggcggtggt gcaacaactg
    11341 ttttaactaa ggatggtatg acaagaggcc cagtagtccg tttcccaact ttgaaaagat
    11401 ctggtgcctg taagatatgg ttagactcag aagagggaca aaacgcaatt aaaaaagctt
    11461 ttaactctac atcaagattt gcacgtctgc aacatattca aacttgtcta gcaggagatt
    11521 tactcttcat gagatttaga acaactactg gtgacgcaat gggtatgaat atgatttcta
    11581 agggtgtcga atactcatta aagcaaatgg tagaagagta tggctgggaa gatatggagg
    11641 ttgtctccgt ttctggtaac tactgtaccg acaaaaaacc agctgccatc aactggatcg
    11701 aaggtcgtgg taagagtgtc gtcgcagaag ctactattcc tggtgatgtt gtcagaaaag
    11761 tgttaaaaag tgatgtttcc gcattggttg agttgaacat tgctaagaat ttggttggat
    11821 ctgcaatggc tgggtctgtt ggtggattta acgcacatgc agctaattta gtgacagctg
    11881 ttttcttggc attaggacaa gatcctgcac aaaatgtcga aagttccaac tgtataacat
    11941 tgatgaaaga agtggacggt gatttgagaa tttccgtatc catgccatcc atcgaagtag
    12001 gtaccatcgg tggtggtact gttctagaac cacaaggtgc catgttggac ttattaggtg
    12061 taagaggccc acatgctacc gctcctggta ccaacgcacg tcaattagca agaatagttg
    12121 cctgtgccgt cttggcaggt gaattatcct tatgtgctgc cctagcagcc ggccatttgg
    12181 ttcaaagtca tatgacccac aacaggaaac ctgctgaacc aacaaaacct aacaatttgg
    12241 acgccactga tataaatcgt ttgaaagatg ggtccgtcac ctgcattaaa tcctaatgat
    12301 aatattgttc gttttttatc atagtttttc aaat
//
