LOCUS       sequence-201303-        7971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  humanized, Neuron-specific promoter driven expression of the 
            rhodopsin guanylyl cyclase from Catenaria anguillulae with a 
            neuron-specific promoter. Useful for raising intracellular cAMP 
            close to the m.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Scheib U, Broser M, Constantin OM, Yang S, Gao S, Mukherjee S, 
            Stehfest K, Nagel G, Gee CE, Hegemann P
  TITLE     Rhodopsin-cyclases for photocontrol of cGMP/cAMP and 2.3 A structure
            of the adenylyl cyclase domain.
  JOURNAL   Nat Commun. 2018 May 24;9(1):2046. doi: 10.1038/s41467-018-04428-w.
  PUBMED    29799525
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-24"; volume: "9(1):2046. doi"; pages: " 
            10.1038/s41467-018-04428-w"
COMMENT     Sequence Label: pAAV hSyn1 CaRhGC wt T2A tDimer
FEATURES             Location/Qualifiers
     source          1..7971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   372..501
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(563..1151)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(643..662)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1322..2113,2114..2182))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1945..1964
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2183..2287)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2355..2373
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2411..2433)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2533..2552
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2569..3024)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2706..2727)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2918..2937
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   3099..3239
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3110..3239
                     /label=AAV2 ITR
     polyA_signal    3279..3755
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     3537..3556
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     3822..3838
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3823..3839
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3842..4430
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3948..3959
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4357..4377
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4458..5132)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     CDS             complement(5175..5852)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     regulatory      5845..5854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5849..5858
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5874..5927)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        complement(join(7847..7971,1..323))
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 gtccggctgg gccggcggcg gcgcggacgc gaccaaggtg gccgggaagg ggagtttgcg
       61 ggggaccggc gagtgacgtc agcgcgcctt cagtgctgag gcggcggtgg cgcgcgccgc
      121 caggcggggg cgaaggcact gtccgcggtg ctgaagctgg cagtgcgcac gcgcctcgcc
      181 gcatcctgtt tcccctcccc ctctctgata ggggatgcgc aatttgggga atgggggttg
      241 ggtgcttgtc cagtgggtcg gggtcggtcg tcaggtaggc acccccaccc cgcctcatcc
      301 tggtcctaaa acccacttgc actcatacgc agggccctct gcagctcgag tctagcgacg
      361 cgtgcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
      421 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggcggcctca gtgagcgagc
      481 gagcgcgcag ctgcctgcag gacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      541 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      601 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      661 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      721 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      781 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      841 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      901 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      961 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1021 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1081 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1141 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1201 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1261 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1321 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1381 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1441 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1501 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1561 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1621 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1681 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1741 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1801 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1861 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1921 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1981 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2041 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2101 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2161 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2281 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     2341 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     2401 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     2461 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     2521 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccataaa attgtaaacg
     2581 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     2641 agaccgaaat cggcaaaatc ccttataaat caaaagaata gcccgagata gagttgagtg
     2701 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     2761 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt
     2821 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     2881 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     2941 gcgctaaggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3001 ttaatgcgcc gctacagggc gcgtactatg gttgctttga cgtatgcggt gtgaaatacc
     3061 gcacagatgc gtaaggagaa aataccgcat caggcgcccc tgcaggcagc tgcgcgctcg
     3121 ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg gtcgcccggc
     3181 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg
     3241 cggccgctcg gtccgcacgt ggttacctac aaaatcagaa ggacagggaa gggagcagtg
     3301 gttcacgcct gtaatcccag caatttggga ggccaaggtg ggtagatcac ctgagattag
     3361 gagttggaga ccagcctggc caatatggtg aaaccccgtc tctaccaaaa aaacaaaaat
     3421 tagctgagcc tggtcatgca tgcctggaat cccaacaact cgggaggctg aggcaggaga
     3481 atcgcttgaa cccaggaggc ggagattgca gtgagccaag attgtgccac tgcactccag
     3541 cttggttccc aatagacccc gcaggcccta caggttgtct tcccaacttg ccccttgctc
     3601 cataccaccc ccctccaccc cataatatta tagaaggaca cctagtcaga caaaatgatg
     3661 caacttaatt ttattaggac aaggctggtg ggcactggag tggcaacttc cagggccagg
     3721 agaggcactg gggaggggtc acagggatgc cacccgtaga tcatctctag actcgaggtc
     3781 gaggccagag aggccatcga atggcgcgcc gcgatcgcgt ctcgaggtcg acggtatcga
     3841 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
     3901 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
     3961 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
     4021 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
     4081 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
     4141 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
     4201 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
     4261 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
     4321 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
     4381 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
     4441 ttttgctcga agtcgaccta caggaacagg tggtggcggc cctcggagcg ctcgtactgt
     4501 tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca gcttggtgtc cacgtagtag
     4561 tagccgggca gctgcacggg cttcttggcc atgtagatgg tcttgaactc caccaggtag
     4621 tggccgccgt ccttcagctt cagggcctgg tggatctcgc ccttcagcac gccgtcgcgg
     4681 gggtacaggc gctcggtgga ggcctcccag cccatggtct tcttctgcat tacggggccg
     4741 tcggggggga agttggtgcc gcggaacttc accttgtaga tcagcgtgcc gtcctgcagg
     4801 gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac
     4861 ttgaagccct cggggaagga cagcttcttg tagtcgggga tgtcggcggg gtgcttcacg
     4921 tacgccttgg agccgtactg gaactggggg gacaggatgt cccaggcgaa gggcaggggg
     4981 ccgcccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc
     5041 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgcgcac cttgaagcgc
     5101 atgaactctt tgatgacgtc ctcggaggag gcggtgccgg agctgccgct gccggtgctg
     5161 ccggtgccat gccccaggaa caggtggtgg cggccctcgg agcgctcgta ctgttccacg
     5221 atggtgtagt cctcgttgtg ggaggtgatg tccagcttgg tgtccacgta gtagtagccg
     5281 ggcagctgca cgggcttctt ggccatgtag atggtcttga actccaccag gtagtggccg
     5341 ccgtccttca gcttcagggc ctggtggatc tcgcccttca gcacgccgtc gcgggggtac
     5401 aggcgctcgg tggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcgggg
     5461 gggaagttgg tgccgcggaa cttcaccttg tagatcagcg tgccgtcctg cagggaggag
     5521 tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag
     5581 ccctcgggga aggacagctt cttgtagtcg gggatgtcgg cggggtgctt cacgtacgcc
     5641 ttggagccgt actggaactg gggggacagg atgtcccagg cgaagggcag ggggccgccc
     5701 ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc
     5761 tcgatctcga actcgtggcc gttcacggag ccctccatgc gcaccttgaa gcgcatgaac
     5821 tctttgatga cgtcctcgga ggaggccacc atggcggccg cggatcccgg tgcagggccg
     5881 ggattctcct ccacgtcacc gcatgttaga agacttcctc tgccctcggc cgcgggtacc
     5941 gactttctag cggtcaccca atatgtctgc atttttccct ttcctttcac ttccatcagg
     6001 tccagtggct gggtcacgaa ttttcccttg atcatctggt aagtagagtc ggagatatga
     6061 atgtgccctg ccttgctggt tgactccatc cgggaagctg tattgacggt atcccccacc
     6121 aggcaccaat gagggttcag gtcgcccagg actccggctg tcactggtcc actattcagg
     6181 ccgatgcgaa tgttgataga ttcgccggta gcagttttaa aagtcttgat catctcgatg
     6241 atgtccaggg cgaagttcac tgctcgatca gcgtggtcgg ggaccacctc tggtgctccg
     6301 gtcactccca gataagcgtc cccaatagtt tcgaccttat acacgcccca ccgcttagcg
     6361 atggcatcat actccagcca cagcttgttc agggtggcca tcatgtcctt tgtggaggtc
     6421 ctggagctaa tcacagtaaa gtttgtgatg tcagaaaaga agactgtcac ggattcgtac
     6481 tctttggcct cggttgcctg tccattcttc agctgttcca gcaccccctc aggcatcatc
     6541 ttctgcagaa tggcgtcaca gttagtgatc tgagattcca gttcctctgc aatagctgtg
     6601 atggcttcga ctttctgatt ctgtgcctgc tccacagtgc tgttgaccag caccagtgtc
     6661 aggaacacct tggccccaat atcgatcatg gtaaaagatg cctcagtcat tgtgaaactc
     6721 agcatgccag atgtaaaggc gaaccagctc agtggaaaca gggtccatgt ggtgacagtt
     6781 gagaagcgtg tccacctcag gccattcact tcaatgttgc aaggggtctc tccgttgatg
     6841 gccccagtaa aggacctcca caggctgtag accacgaagc taaagaaggc acatgacagg
     6901 atattaaaca ggtttcccca gggataaggg ggcagcacag ctccaaagaa cccgcagacc
     6961 accagggcgt aatcagagaa gaccaccccc caggcattat ggtctgcctg tgtaatctcg
     7021 gagatcagca gaatcaggac gggacaggta gcaatccact ccaggtatct tgcaggctcc
     7081 actgggttgc tcagggtgcc ttcgatggtt ggagtcagcc gcagcataat caggatgtat
     7141 gaactaaaag cgatgatgtt cactcccagc aggcacaggg acagtgcttt cttctctggg
     7201 tttctggcgc ctgcaaatcc catataactg aaagcaatcc cgatgccaaa tccgaaggcc
     7261 ccatagccgt accaaatgtc tgcccagccc ctattataag cctgtctttc ctggataaac
     7321 agagcgtaga aagcgccggc cacggtcaga atggcccaca tgataaactg tcctgtcagg
     7381 aatttcagtg aaggggtact cagcatcata tcccaggacc acatgtctgc gatgtttgtc
     7441 tggtactgct gcagagcgga ctgcttacta ggagagccag gtcggctatt ggcgggagag
     7501 gacacgttgc ttgaacttgc agaggaggtg ctgcggacat tggacacgct cagtgaattg
     7561 ttctgtctgt tccgcatatt agcgttgctt gaggcggtac tagagccagt gaccttgata
     7621 ttgggctgac tgggagatgg cagcagtgag gctccggagc tgcggaaact tccgtttcga
     7681 gtcagtggct gctcgacgat agggtgtttt gtcacgctac acttgcaatc gtcgcacagg
     7741 gttgaggttg gtgagtaaca gtattcaggg caggaacagc cactacatgc tccccgcagg
     7801 ttattatctt tatctttcat agacatagac attattgcga attcgactgc gctctcaggc
     7861 acgacacgac tcctccgctg cccaccgcag actgaggcag cgctgagtcg ccggcgccgc
     7921 agcgcagatg gtcgcgcccg tgccccccta tctcgcgcct cgcgtggtgc g
//
