LOCUS       sequence-201305-        7138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yellow fluorescent protein (YFP) – tagged rhodopsin adenylyl cyclase
            created from the rhodopsin guanylyl cyclase of Catenaria anguillulae
            with a neuron-specific promoter. Useful for raising intracellu.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Scheib U, Broser M, Constantin OM, Yang S, Gao S, Mukherjee S, 
            Stehfest K, Nagel G, Gee CE, Hegemann P
  TITLE     Rhodopsin-cyclases for photocontrol of cGMP/cAMP and 2.3 A structure
            of the adenylyl cyclase domain.
  JOURNAL   Nat Commun. 2018 May 24;9(1):2046. doi: 10.1038/s41467-018-04428-w.
  PUBMED    29799525
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-24"; volume: "9(1):2046. doi"; pages: " 
            10.1038/s41467-018-04428-w"
COMMENT     Sequence Label: pAAV hSyn1 YFP-CaRhAC
FEATURES             Location/Qualifiers
     source          1..7138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   423..552
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     rep_origin      complement(614..1202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(694..713)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1373..2164,2165..2233))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1996..2015
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2234..2338)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2406..2424
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2462..2484)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2584..2603
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2620..3075)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2757..2778)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2969..2988
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   3150..3290
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3161..3290
                     /label=AAV2 ITR
     polyA_signal    3330..3806
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     3588..3607
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     misc_feature    3838..4426
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3944..3955
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4353..4373
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(6327..7037,7038..7040,7041..7043))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6369..6390)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6718..6737
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6977..6998
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      7040..7049
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(7069..7138,1..378))
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 ccgcagcgca gatggtcgcg cccgtgcccc cctatctcgc gcctcgcgtg gtgcggtccg
       61 gctgggccgg cggcggcgcg gacgcgacca aggtggccgg gaaggggagt ttgcggggga
      121 ccggcgagtg acgtcagcgc gccttcagtg ctgaggcggc ggtggcgcgc gccgccaggc
      181 gggggcgaag gcactgtccg cggtgctgaa gctggcagtg cgcacgcgcc tcgccgcatc
      241 ctgtttcccc tccccctctc tgatagggga tgcgcaattt ggggaatggg ggttgggtgc
      301 ttgtccagtg ggtcggggtc ggtcgtcagg taggcacccc caccccgcct catcctggtc
      361 ctaaaaccca cttgcactca tacgcagggc cctctgcagt ctagacacac gcgtgcggcc
      421 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
      481 ggccgggcga ccaaaggtcg cccgacgccc gggcggcctc agtgagcgag cgagcgcgca
      541 gctgcctgca ggacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      601 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      661 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      721 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      781 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      841 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      901 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      961 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1021 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1081 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1141 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1201 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1261 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1321 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1381 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1441 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1501 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1561 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1621 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1681 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1741 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1801 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1861 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1921 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1981 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2041 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2101 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2161 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2221 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2281 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2341 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     2401 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     2461 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     2521 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     2581 atgcggcatc agagcagatt gtactgagag tgcaccataa aattgtaaac gttaatattt
     2641 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa tagaccgaaa
     2701 tcggcaaaat cccttataaa tcaaaagaat agcccgagat agagttgagt gttgttccag
     2761 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     2821 tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga
     2881 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     2941 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaagg
     3001 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     3061 cgctacaggg cgcgtactat ggttgctttg acgtatgcgg tgtgaaatac cgcacagatg
     3121 cgtaaggaga aaataccgca tcaggcgccc ctgcaggcag ctgcgcgctc gctcgctcac
     3181 tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag
     3241 cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct gcggccgctc
     3301 ggtccgcacg tggttaccta caaaatcaga aggacaggga agggagcagt ggttcacgcc
     3361 tgtaatccca gcaatttggg aggccaaggt gggtagatca cctgagatta ggagttggag
     3421 accagcctgg ccaatatggt gaaaccccgt ctctaccaaa aaaacaaaaa ttagctgagc
     3481 ctggtcatgc atgcctggaa tcccaacaac tcgggaggct gaggcaggag aatcgcttga
     3541 acccaggagg cggagattgc agtgagccaa gattgtgcca ctgcactcca gcttggttcc
     3601 caatagaccc cgcaggccct acaggttgtc ttcccaactt gccccttgct ccataccacc
     3661 cccctccacc ccataatatt atagaaggac acctagtcag acaaaatgat gcaacttaat
     3721 tttattagga caaggctggt gggcactgga gtggcaactt ccagggccag gagaggcact
     3781 ggggaggggt cacagggatg ccacccgtag atctctcgag cagcgctcgg tatcgatgcg
     3841 gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag
     3901 gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga
     3961 cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac ataggcgagc agccaaggaa
     4021 aggacgatga tttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg
     4081 tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag
     4141 cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt
     4201 cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa
     4261 gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag
     4321 catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa
     4381 gaataccagt caatctttca caaattttgt aatccagagg ttgattatcg ataagcttat
     4441 cactttcggg ctgtgaccca gtatgtttgc atctttccct ttcccttcac ttccatgaga
     4501 tcgagtggtt gtgtcacgaa ctttcccttg atcatttggt atgtcgaatc cgagatgtgg
     4561 atgtgtccag cctttgaagt cgattccatt cgcgaggcag tattcactgt atctcctacg
     4621 aggtcccagt gtggattcaa atctccaaga actccggctg taactggtcc cgagttgagt
     4681 ccgattcgga tgttgatcga ctctccagtg gctgtcttga atgtcttgat catctcgatg
     4741 atatcgaggg cgaagttaac agctcgatca gcatgatctg gcacaacctc tggtgctcca
     4801 gtaactccga ggtaagcatc tccgattgtc ttgaccttgt acactcccca tcgtttagcg
     4861 atagcatcgt attcgagcca gagcttgttg agtgtggcca tcatatcttt tgtactagtt
     4921 cgcgacgaga tcactgtgaa gttagtgata tccgagaaga acactgtgac tgactcgtac
     4981 tctttggcct ctgtggcttg tccattcttg agttgttcga gaactccctc tggcatcatc
     5041 ttttggagga tggcatcaca gtttgtgatt tgcgattcga gttcttcggc gatagctgtg
     5101 atggcctcga ccttttgatt ttgggcttgc tcgactgtcg agttaacgag aacgagagtg
     5161 aggaacacct tggctccgat atcgatcata gtgaatgagg cctctgtcat tgtgaacgag
     5221 agcattcccg atgtgaaggc gaaccaactg agtgggaaga gtgtccaagt tgtcactgtc
     5281 gagaatcgtg tccatcggag tccgttcact tcgatgttgc atggtgtttc tccgttgata
     5341 gctccagtga acgatcgcca gagtgaatac accacgaacg agaagaaagc gcacgagaga
     5401 atgttgaaga ggtttcccca tgggtatggt ggaagaacag ctccgaagaa tccgcacaca
     5461 acaagagcgt aatctgagaa caccactccc caagcgttgt gatcagcttg tgtaatctcc
     5521 gagatgagca ggatgagcac tgggcatgta gcaatccatt cgaggtatcg agctggctca
     5581 actgggtttg agagtgttcc ctcgatagtt ggtgtgagtc ggagcatgat gaggatgtac
     5641 gaactgaagg cgataatgtt cactccgagg aggcaaagcg aaagggcttt cttttctggg
     5701 tttctagctc ctgcgaatcc catgtacgag aaggcgattc cgattccgaa tccaaaagct
     5761 ccgtatccgt accaaatatc ggcccatcct cgattgtagg cttgtcgttc ttggatgaag
     5821 agagcgtaga aagctccggc aactgtgaga atggcccaca tgatgaattg tcctgtgagg
     5881 aacttgagtg atggtgttga gagcatcata tcccacgacc acatatcggc aatgtttgtt
     5941 tggtattgtt ggagtgccga ttgttttgat ggcgatcctg gtcgcgaatt agctggcgac
     6001 gaaacatttg acgacgaggc cgatgatgtc gatcgcacgt ttgacaccga gagcgagttg
     6061 ttttgtcggt ttcgcatatt agcattcgac gaggctgtcg acgatcctgt cactttgatg
     6121 tttggttgtg atggtgatgg aagaagtgaa gctcccgacg atctgaacga tccgtttcgt
     6181 gtgagtggtt gctcaacgat tgggtgcttt gtgaccgagc acttgcaatc atcacagagt
     6241 gtcgatgttg gcgagtagca atattctggg catgaacatc ctgagcaggc tccacggaga
     6301 ttgttatcct tatccttctt ctcgagcttg tacagctcgt ccatgccgag agtgatcccg
     6361 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
     6421 agggcggact ggtagctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
     6481 ggggtgttct gctggtagtg gtcggcgagc tgcacgccgc cgtcctcgat gttgtggcgg
     6541 atcttgaagt tggccttgat gccgttcttc tgcttgtcgg cggtgatata gacgttgtgg
     6601 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
     6661 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
     6721 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
     6781 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa gcactgcagg
     6841 ccgtagccca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
     6901 atcagcttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
     6961 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
     7021 agctcctcgc ccttgctcac catggtggcg gatccggtac cttctcgact gcgctctcag
     7081 gcacgacacg actcctccgc tgcccaccgc agactgaggc agcgctgagt cgccggcg
//
