LOCUS       sequence-201309-        8242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High efficiency channelrhodopsin with cytomegalovirus enhancer fused
            to chicken beta-actin (CAG) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Berndt A, Schoenenberger P, Mattis J, Tye KM, Deisseroth K, Hegemann
            P, Oertner TG
  TITLE     High-efficiency channelrhodopsins for fast neuronal stimulation at 
            low light levels.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 3;108(18):7595-600. doi: 
            10.1073/pnas.1017210108. Epub 2011 Apr 19.
  PUBMED    21504945
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1017210108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-3- 3"; volume: "108"; issue: "18"; pages: "7595-600"
COMMENT     Sequence Label: pAAV CAG ChR2 E123T T159C 2A tDimer
FEATURES             Location/Qualifiers
     source          1..8242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1673
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1597..1619
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1681..1700
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1737..1746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1743..2669
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYATWLLTCPVILIHLSNLTGLSNDYSRRTMGLLVSDIGCIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             2679..2732
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2748..2757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2752..2761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2754..3431
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             3474..4148
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     misc_feature    4176..4764
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4229..4249)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4647..4658)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4767..4783)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4768..4784)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    4851..5327
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5050..5069)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5367..5507
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5367..5496
                     /label=AAV2 ITR
     rep_origin      5582..6037
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5669..5688)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5879..5900
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6054..6073)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6173..6195
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6233..6251)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6319..6423
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6424..6492,6493..7284)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6642..6661)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7455..8043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7944..7963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   8105..8234
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 actcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
     1441 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     1501 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
     1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     1681 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcaag ctgctagcca
     1741 ccatggatta tggaggcgcc ctgagtgccg ttgggcgcga gctgctattt gtaacgaacc
     1801 cagtagtcgt caatggctct gtacttgtgc ctgaggacca gtgttactgc gcgggctgga
     1861 ttgagtcgcg tggcacaaac ggtgcccaaa cggcgtcgaa cgtgctgcaa tggcttgctg
     1921 ctggcttctc catcctactg cttatgtttt acgcctacca aacatggaag tcaacctgcg
     1981 gctgggagga gatctatgtg tgcgctatcg agatggtcaa ggtgattctc gagttcttct
     2041 tcgagtttaa gaacccgtcc atgctgtatc tagccacagg ccaccgcgtc cagtggttgc
     2101 gttacgccac gtggcttctc acctgcccgg tcattctcat tcacctgtca aacctgacgg
     2161 gcttgtccaa cgactacagc aggcgcacca tgggtctgct tgtgtctgat attggctgta
     2221 ttgtgtgggg cgccacttcc gccatggcca ccggatacgt caaggtcatc ttcttctgcc
     2281 tgggtctgtg ttatggtgct aacacgttct ttcacgctgc caaggcctac atcgagggtt
     2341 accacaccgt gccgaagggc cggtgtcgcc aggtggtgac tggcatggct tggctcttct
     2401 tcgtatcatg gggtatgttc cccatcctgt tcatcctcgg ccccgagggc ttcggcgtcc
     2461 tgagcgtgta cggctccacc gtcggccaca ccatcattga cctgatgtcg aagaactgct
     2521 ggggtctgct cggccactac ctgcgcgtgc tgatccacga gcatatcctc atccacggcg
     2581 acattcgcaa gaccaccaaa ttgaacattg gtggcactga gattgaggtc gagacgctgg
     2641 tggaggacga ggccgaggct ggcgcggtac ccgcggccga gggcagagga agtcttctaa
     2701 catgcggtga cgtggaggag aatcccggcc ctgcaccggg atccgcggcc gccatggtgg
     2761 cctcctccga ggacgtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctccg
     2821 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     2881 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     2941 ccccccagtt ccagtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgact
     3001 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     3061 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg
     3121 tgaagttccg cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg
     3181 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     3241 accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca
     3301 tggccaagaa gcccgtgcag ctgcccggct actactacgt ggacaccaag ctggacatca
     3361 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc
     3421 acctgttcct ggggcatggc accggcagca ccggcagcgg cagctccggc accgcctcct
     3481 ccgaggacgt catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg
     3541 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     3601 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc
     3661 agttccagta cggctccaag gcgtacgtga agcaccccgc cgacatcccc gactacaaga
     3721 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     3781 tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaagt
     3841 tccgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg
     3901 aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg
     3961 ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca
     4021 agaagcccgt gcagctgccc ggctactact acgtggacac caagctggac atcacctccc
     4081 acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt
     4141 tcctgtaggt cgacttcgag caaaagctta tcgataatca acctctggat tacaaaattt
     4201 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4261 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4321 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4381 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4441 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4501 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4561 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4621 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4681 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4741 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagacgcg atcgcggcgc
     4801 gccattcgat ggcctctctg gcctcgacct cgagtctaga gatgatctac gggtggcatc
     4861 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca
     4921 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
     4981 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg
     5041 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
     5101 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc
     5161 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca
     5221 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca
     5281 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg
     5341 taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc
     5401 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     5461 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
     5521 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     5581 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5641 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5701 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5761 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     5821 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5881 ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta
     5941 taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt
     6001 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
     6061 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     6121 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     6181 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     6241 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     6301 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6361 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6421 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6481 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6541 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6601 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6661 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6721 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6781 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6841 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6901 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6961 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7021 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7081 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7141 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7201 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7261 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7321 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7381 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7441 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7501 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7561 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7621 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7681 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7741 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7801 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7861 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7921 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7981 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8041 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8101 atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg ccgcccgggc gtcgggcgac
     8161 ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat
     8221 cactaggggt tcctgcggcc gc
//
