LOCUS       sequence-201330-        9052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic promoter CEBPB drives mKate2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9052)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 9052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN380 (FuGW-S(CEBPB)p-mKate2)
FEATURES             Location/Qualifiers
     source          1..9052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..88
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(97..525)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(207..228)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     419..438
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    571..795
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     784..801
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             827..1007
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1512..1528
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1513..1529
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1532..2120
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1638..1649
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2047..2067
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      2560..2569
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2566..3279
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    3379..3496
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3794..3838)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4023..4256
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4749..4874
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4921..5101
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5119..5317)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5144..5164)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5319..5698
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5870..5889
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5964..6068
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6069..6137,6138..6929)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6287..6306)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7100..7688
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7589..7608
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7842..7859
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7976..7997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8012..8017,8018..8035,8036..8042)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8050..8066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8055..8077
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8074..8090
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8074..8090
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8139..8260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8150..8169)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8204..8223
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8390..8764)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(8783..8830)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      8892..9027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8946..8965)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
       61 tttccacacc ctaactgaca cacattccac agaattaatt cgcgttaaat ttttgttaaa
      121 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
      181 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
      241 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
      301 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
      361 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
      421 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
      481 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtgggga taccccctag
      541 agccccagct ggttctttcc gcctcagaag ccatagagcc caccgcatcc ccagcatgcc
      601 tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag
      661 aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg
      721 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc
      781 aactagaagg cacagtcgag gctgatcagc gggtttaaac gggccctgct agagattttc
      841 cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca
      901 cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt
      961 tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccagt acagtccgga
     1021 tgcagctctc gggccatgtg atgaaatgct aggcggctgt caaacctcca ctctaatact
     1081 tctctctccg ggtcatccat cccatgcagg ctcacagggt gtaacaagcg ggtgttctct
     1141 ccttcattgg cttcttctac cttctcttgc tcaactggta ctagcttgta gcaccatcca
     1201 aaggtcagtg gatatctgat ccctggccct ggtgtgtagt tctgccaatc agggaagtag
     1261 ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc ttcgttggga gtgaattagc
     1321 ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc
     1381 attggtctta aaggtacctg aggtgtgact ggaaaaccca cctcctcctc ctcttgtgct
     1441 tctagccagg cacaatcagc attggtagct gctgtattgc tacttgtgat tgctccatgt
     1501 ttttctaggt ctcgaggtcg acggtatcga tgcggggagg cggcccaaag ggagatccga
     1561 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg
     1621 gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga
     1681 atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc cgacaacacc
     1741 acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg
     1801 atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag
     1861 gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca
     1921 cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag
     1981 gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg
     2041 tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt
     2101 ttgtaatcca gaggttgatt atcgataagc ttgatatcgc ggccgccctg cacctgcagg
     2161 tctgatgcaa tagcggtatg atgcaaggaa tcagattgtg aaatgtgaca ttgtgaaata
     2221 acttctgatg caatgtcggt atgatgcaag gagagagatt gtgaaatgtt ccattgtgaa
     2281 atacgaattc tgccctactg acactgcctg ccgtctgatg caatagcggt atgatgcaag
     2341 gaatcagatt gtgaaatgtg acattgtgaa ataacttctg atgcaatgtc ggtatgatgc
     2401 aaggagagag attgtgaaat gttccattgt gaaatacgaa tatccgcggc gcgccagacg
     2461 ctagcggggg gctataaaag ggggtggggg cgttcgtcct cactctagat ctgcgatcta
     2521 agtaagcttg gcattccggt actgttggta aaccagctcg ccaccatggt gtctaagggc
     2581 gaagagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac
     2641 caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
     2701 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc
     2761 ttcatgtacg gcagcaaaac cttcatcaac cacacccagg gcatccccga cttctttaag
     2821 cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
     2881 ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
     2941 agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag
     3001 gcctccaccg agatgctgta ccccgctgac ggcggcctgg aaggcagaag cgacatggcc
     3061 ctgaagctcg tgggcggggg ccacctgatc tgcaacttga agaccacata cagatccaag
     3121 aaacccgcta agaacctcaa gatgcccggc gtctactatg tggacagaag actggaaaga
     3181 atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt ggccagatac
     3241 tgcgacctcc ctagcaaact ggggcacaaa cttaattgat aaaccggttg cagtcccggg
     3301 taagcgggac tctggggttc gaaatgaccg accaagcatt aaccaaactg gatctctgct
     3361 gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt
     3421 ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc
     3481 cccttttctt ttaaaattgt ggatgaatac tgccatttgt ctgcagaatt ggcgcacgca
     3541 gtgccgatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt
     3601 ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg
     3661 gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac
     3721 ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa tttgttatgt
     3781 taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt
     3841 cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt
     3901 ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc
     3961 aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc
     4021 ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct
     4081 tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat
     4141 tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg
     4201 acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc
     4261 ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta
     4321 atggttcaat ttttactact ttatatttat ataattcact tctccaattg tccctcatat
     4381 ctcctcctcc aggtctgaag atcagcggcc gcttgctgtg cggtggtctt acttttgttt
     4441 tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat cctttgatgc
     4501 acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga tcctgtctga
     4561 agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc taacaggcca
     4621 ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt taatttatat
     4681 tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat tctcccccgc
     4741 ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt caaaattttt
     4801 ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc aagccgagtc
     4861 ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt cgggcgccac
     4921 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
     4981 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
     5041 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
     5101 cagtacaggc aaaacgcgct gcttatatag acctcccacc gtacacgcct accgcccatt
     5161 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     5221 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     5281 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     5341 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     5401 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     5461 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     5521 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     5581 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     5641 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
     5701 cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc
     5761 ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac
     5821 tcagcgacct ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag
     5881 cagattgtac tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag
     5941 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6001 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6061 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6121 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6181 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6241 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6301 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6361 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6421 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6481 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6541 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6601 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6661 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6721 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6781 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6841 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6901 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6961 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7021 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7081 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7141 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7201 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7261 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7321 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7381 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7441 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7501 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7561 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7621 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7681 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7741 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7801 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7861 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7921 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7981 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     8041 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     8101 ccaagctcta gctagaggtc gacggtatac agacatgata agatacattg atgagtttgg
     8161 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     8221 tgctttattt gtaaccatta taagctgcaa taaacaagtt ggggtgggcg aagaactcca
     8281 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
     8341 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tagcacgtgt cagtcctgct
     8401 cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc
     8461 acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca
     8521 cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg
     8581 tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga
     8641 ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga
     8701 actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg
     8761 ccatggttta gttcctcacc ttgtcgtatt atactatgcc gatatactat gccgatgatt
     8821 aattgtcaac acgtgctgat cagatccgaa aatggatata caagctcccg ggagcttttt
     8881 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggcaga
     8941 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     9001 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tg
//
