LOCUS       sequence-20134-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Hartmann C, Tabin CJ
  TITLE     Wnt-14 plays a pivotal role in inducing synovial joint formation in 
            the developing appendicular skeleton.
  JOURNAL   Cell. 2001 Feb 9. 104(3):341-51.
  PUBMED    11239392
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2001 
            Feb 9. 104(3):341-51."
COMMENT     Sequence Label: pGEM cCol2 (CT#617)
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(546..564)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(547..564)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(582..598)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(582..598)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(595..617)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    606..622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(630..636,637..654,655..660))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    675..696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gctcccggcc gccatggcgg ccgcgggaat tcgattaagg acgtgtggaa gccggagccg
       61 tgccggatct gcgtgtgcga caccgggacc gttctgtgcg acgagatcat ctgcgaggag
      121 ccgcaggact gccccagccc cgaaatcccc ttcggagagt gctgccccgt ctgccccgca
      181 gagctgcccg ccgccgcccg acaacctggc cccaagggac agaagggaga acccggagat
      241 attaaagatg ttgtaggacc ccgagggcct ccaggaccac agggcccagc aggagagcag
      301 ggacagcgag gggaccgtgg cgagaagggg gagaagggtg ctcctggccc ccgtggaagg
      361 gatggagaac ccggcacccc tggaaaccca ggcccccccg gtccccccgg acctcctggc
      421 ccccccggac ttggtggaaa ctttgcggcg cagatggcgg gcggcaatca ctagtgaatt
      481 cgcggccgcc tgcaggtcga ccatatggga gagctcccaa cgcgttggat gcatagcttg
      541 agtattctat agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg
      601 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      661 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      721 ttccagtcgg gaaacctgtc gtgccagctg
//
