LOCUS       sequence-201368-        6245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Ballard DW, Dixon EP, Peffer NJ, Bogerd H, Doerre S, Stein B, Greene
            WC
  TITLE     The 65-kDa subunit of human NF-kappa B functions as a potent 
            transcriptional activator and a target for v-Rel-mediated 
            repression.
  JOURNAL   Proc Natl Acad Sci U S A. 1992 Mar 1. 89(5):1875-9.
  PUBMED    1542686
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1992 Mar 1. 89(5):1875-9."
COMMENT     Sequence Label: pCMV4 p50
FEATURES             Location/Qualifiers
     source          1..6245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     120..142
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     134..151
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     135..151
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        312..691
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        692..895
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     846..866
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(2606..2625)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3053..3073)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3058..3387
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3238..3373
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          3426..3522
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     complement(3598..3617)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3599..3617)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3631..3647)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3631..3647)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3644..3666)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3655..3671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3679..3685,3686..3703,3704..3709))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3724..3745
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3862..3879)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4033..4621)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4113..4132)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4792..5583,5584..5652))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5415..5434
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5653..5757)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5784..6239
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5871..5890)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6081..6102
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
       61 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      121 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg atctatacat tgaatcaata
      181 ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg gctattggcc
      241 attgcatacg ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg
      301 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      361 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      421 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      481 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      541 ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa
      601 atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta
      661 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg
      721 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      781 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc
      841 gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
      901 agtgaaccgt cagaattgtt tttattttta attttctttc aaatacttcc atcgaattca
      961 gatctggtac cacgcgtatc gataagcttg tcgacggatg gcagaagatg atccatattt
     1021 gggaaggcct gaacaaatgt ttcatttgga tccttctttg actcatacaa tatttaatcc
     1081 agaagtattt caaccacaga tggcactgcc aacagcagat ggcccatacc ttcaaatatt
     1141 agagcaacct aaacagagag gatttcgttt ccgttatgta tgtgaaggcc catcccatgg
     1201 tggactacct ggtgcctcta gtgaaaagaa caagaagtct taccctcagg tcaaaatctg
     1261 caactatgtg ggaccagcaa aggttattgt tcagttggtc acaaatggaa aaaatatcca
     1321 cctgcatgcc cacagcctgg tgggaaaaca ctgtgaggat gggatctgca ctgtaactgc
     1381 tggacccaag gacatggtgg tcggcttcgc aaacctgggt atacttcatg tgacaaagaa
     1441 aaaagtattt gaaacactgg aagcacgaat gacagaggcg tgtataaggg gctataatcc
     1501 tggactcttg gtgcaccctg accttgccta tttgcaagca gaaggtggag gggaccggca
     1561 gctgggagat cgggaaaaag agctaatccg ccaagcagct ctgcagcaga ccaaggagat
     1621 ggacctcagc gtggtgcggc tcatgtttac agcttttctt ccggatagca ctggcagctt
     1681 cacaaggcgc ctggaacccg tggtatcaga cgccatctat gacagtaaag cccccaatgc
     1741 atccaacttg aaaattgtaa gaatggacag gacagctgga tgtgtgactg gaggggagga
     1801 aatttatctt ctttgtgaca aagttcagaa agatgacatc cagattcgat tttatgaaga
     1861 ggaagaaaat ggtggagtct gggaaggatt tggagatttt tcccccacag atgttcatag
     1921 acaatttgcc attgtcttca aaactccaaa gtataaagat attaatatta caaaaccagc
     1981 ctctgtgttt gtccagcttc ggaggaaatc tgacttggaa actagtgaac caaaaccttt
     2041 cctctactat cctgaaatca aagataaaga agaagtgcag aggaaacgtc agaagctcat
     2101 gcccaatttt tcggatagtt tcggcggtgg tagtggtgcc ggagctggag gcggaggcat
     2161 gtttggtagt ggcggtggag gagggggcac tggaagtaca ggtccagggt atagcttccc
     2221 acactatgga tttcctactt atggtgggat tactttccat cctggaacta ctaaatctaa
     2281 tgctgggatg aagcatggaa ccatggacac tgaatctaaa aaggaccctg aaggttgtga
     2341 caaaagtgat gacaaaaaca ctgtaaacct ctttgggaaa gatcctagag gatccttaag
     2401 ttaacttaat ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
     2461 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     2521 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
     2581 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
     2641 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
     2701 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
     2761 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
     2821 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     2881 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct
     2941 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt
     3001 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcttggct gtggaatgtg
     3061 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3121 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     3181 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     3241 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3301 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     3361 ttttttggag gcctaggctt ttgcaaaaag ctcctcgagg aactgaaaaa ccagaaagtt
     3421 aactggtaag tttagtcttt ttgtctttta tttcaggtcc cggatccggt ggtggtgcaa
     3481 atcaaagaac tgctcctcag tggatgttgc ctttacttct aggcctgtac ggaagtgtta
     3541 cttctgctct aaaagctgct gcaggtcgac tctagggccg cgtaccgagc tcgaattccc
     3601 tatagtgagt cgtattaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     3661 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3721 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3781 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3841 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3901 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3961 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4021 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4081 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4141 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4201 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4261 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4321 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4381 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4441 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4501 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4561 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4621 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4681 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4741 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4801 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4861 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4921 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4981 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     5041 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     5101 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     5161 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     5221 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     5281 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5341 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     5401 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5461 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5521 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5581 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5641 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5701 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5761 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5821 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5881 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5941 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6001 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6061 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6121 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6181 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc
     6241 cattc
//
