LOCUS       sequence-201370-       14353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Triple gRNA expression separated with 28nt Csy4 motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14353)
  AUTHORS   Ferreira R, Skrekas C, Nielsen J, David F
  TITLE     Multiplexed CRISPR/Cas9 Genome Editing and Gene Regulation using 
            Csy4 in Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2017 Nov 21. doi: 10.1021/acssynbio.7b00259.
  PUBMED    29161506
REFERENCE   2  (bases 1 to 14353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Nov 21. doi: 10.1021/acssynbio.7b00259."
COMMENT     Sequence Label: pCRA03
FEATURES             Location/Qualifiers
     source          1..14353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        355..459
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(460..528,529..1320)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(678..697)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1491..2079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1980..1999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2474..2492
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2558..2577
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        2606..2680
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    2685..2704
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        2733..2807
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    2812..2831
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        2860..2934
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    2939..2958
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     terminator      2967..3029
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     CDS             4164..4787
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4237..4256)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     5284..5301
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5418..5439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5454..5459,5460..5477,5478..5484)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5492..5508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5497..5519
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5516..5532
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5516..5532
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5551..5571
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5553..5571
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5589..5987
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             6010..6030
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(6040..6066,6067..6069,6070..8553,8554..8559,8560..8625,
                     8626..8628,8629..10143)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(6040..6066,6067..6069,6070..8556,8557..8559,
                     8560..10143)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6040..10143
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10156..10176
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10201..10350
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             10375..10395
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10825..11181
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             11200..11769
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     protein_bind    complement(11774..11798)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11831..11847)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11832..11848)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      11850..12097
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(11866..11884)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(12115..12134)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12116..12134)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12144..12161)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12144..12160)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12153..12175)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12301..12756
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12388..12407)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12598..12619
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(12887..13690)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(13691..13906)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(13930..13949)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14049..14071
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(14109..14127)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    join(14168..14353,1..318)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat acattcgatt
       61 aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac acagacaaga
      121 tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg tagtatttgt
      181 tggcgatccc cctagagtct tttacatctt cggaaaacaa aaactatttt ttctttaatt
      241 tcttttttta ctttctattt ttaatttata tatttatatt aaaaaattta aattataatt
      301 atttttatag cacgtgatga aaaggaccca ggtggcactt ttcggggaaa tgtgcgcgga
      361 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      421 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
      481 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
      541 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
      601 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
      661 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
      721 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
      781 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
      841 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
      901 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
      961 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     1021 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     1081 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     1141 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     1201 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     1261 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     1321 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     1381 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     1441 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1501 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1561 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1621 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     1681 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1741 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1801 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1861 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1921 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1981 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2041 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2101 ttacggttcc tggccttttg ctggcctttt gctggccttt tgctcggggg atctgccaat
     2161 tgaacataac atggtagtta catatactag taatatggtt cggcacacat taaaagtata
     2221 aaaactatct gaattacgaa ttacatatat tggtcataaa aatcaatcaa tcatcgtgtg
     2281 ttttatatgt ctcttatcta agtataagaa tatccatagt taatattcac ttacgctacc
     2341 ttttaacctg taatcattgt caacaggata tgttaacgac ccacattgat aaacgctagt
     2401 atttcttttt cctcttctta ttggccggct gtctctatac tcccctatag tctgtttctt
     2461 ttcgtttcga ttgtccctat cagtgataga gatggcgcac atggtacgct gtggtgctcg
     2521 cggctgggaa cgaaactctg ggagctgcga ttggcaggtt cactgccgta taggcagcta
     2581 agaaacgccc cagttggctt ggctcgtttt agagctagaa atagcaagtt aaaataaggc
     2641 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg gtgcgttcac tgccgtatag
     2701 gcagctaaga aaagctgttg ttcgataaat ccgttttaga gctagaaata gcaagttaaa
     2761 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtggtg cgttcactgc
     2821 cgtataggca gctaagaaat aatggcatcg gaggctcggg ttttagagct agaaatagca
     2881 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtggtgcgt
     2941 tcactgccgt ataggcagct aagaaaccat atccaacttc caatttaatc tttctttttt
     3001 aattttcact tatttgcgat acagaaagaa aaaagcgata gtaactattg aattttgttt
     3061 ggatttggtt agattagata tggtttctct ttatatttac atgctaaaaa tgggctacac
     3121 cagagataca taattagata tatatacgcc agtacacctt atcggcccaa gccttgtccc
     3181 aaggcagcgt tttgttcttg gaaacgctgc cctacacgtt cgctatgctt caagaacttt
     3241 tctgagcact tcatgatgca tgtttgttcc ttattggtta gctttgatgt tgtgaagtca
     3301 ttgacacagt ctgtgaaaca tctttctacc agattagagt acaaacgcat gaaatccttc
     3361 atttgctttt gttccactac tttttggaac tcttgttgtt ctttcctgca ggtagtcgtg
     3421 caatgtatga ctttaagatt tgtgagcagg aagaaaaggg agaatcttct aacgataaac
     3481 ccttgaaaaa ctgggtagac tacgctatgt tgagttgcta cgcaggctgc acaattacac
     3541 gagaatgctc ccgcctagga tttaaggcta agggacgtgc aatgcagacg acagatctaa
     3601 atgaccgtgt cggtgaagtg ttcgccaaac ttttcggtta acacatgcag tgatgcacgc
     3661 gcgatggtgc taagttacat atatatatat atgtatagcc atagtgatgt ctaagtaacc
     3721 tttatggtat atttcttaat gtggaaagat actagcgcgc gcacccacac acaagcttcg
     3781 tcttttcttg aagaaaagag gaagctcgct aaatgggatt ccactttccg ttccctgcca
     3841 gctgatggaa aaaggttagt ggaacgatga agaataaaaa gagagatcca ctgaggtgaa
     3901 atttcagctg acagcgagtt tcatgatcgt gatgaacaat ggtaacgagt tgtggctgtt
     3961 gccagggagg gtggttctca acttttaatg tatggccaaa tcgctacttg ggtttgttat
     4021 ataacaaaga agaaataatg aactgattct cttcctcctt cttgtccttt cttaattctg
     4081 ttgtaattac cttcctttgt aatttttttt gtaattattc ttcttaataa tccaaacaaa
     4141 cacacatatt acaatatatt aaaatgtcta gattagataa aagtaaagtg attaacagcg
     4201 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
     4261 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
     4321 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
     4381 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
     4441 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
     4501 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
     4561 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
     4621 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
     4681 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
     4741 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtcttaagtc tgaagaatga
     4801 atgatttgat gatttctttt tccctccatt tttcttactg aatatatcaa tgatatagac
     4861 ttgtatagtt tattatttca aattaagtag ctatatatag tcaagataac gtttgtttga
     4921 cacgattaca ttattcgtcg acatcttttt tcagcctgtc gtggtagcaa tttgaggagt
     4981 attattaatt gaataggttc attttgcgct cgcataaaca gttttcgtca gggacagtat
     5041 gttggaatga gtggtaatta atggtgacat gacatgttat agcaataacc ttgatgttta
     5101 catcgtagtt taatgtacac cccgcgaatt cgttcaagta ggagtgcacc aattgcaaag
     5161 ggaaaagctg aatgggcagt tcgaaccgcg gtctttcctg cgttatcccc tgattctgtg
     5221 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     5281 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     5341 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     5401 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     5461 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     5521 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     5581 ctggagctca tagcttcaaa atgtttctac tcctttttta ctcttccaga ttttctcgga
     5641 ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca gcatactaaa tttcccctct
     5701 ttcttcctct agggtgtcgt taattacccg tactaaaggt ttggaaaaga aaaaagagac
     5761 cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt
     5821 cttgaaaatt ttttttttga tttttttctc tttcgatgac ctcccattga tatttaagtt
     5881 aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgttcta ttacaacttt
     5941 ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt tctagagatg
     6001 tctagagccc caaagaagaa gagaaaagtt agacccggga tggacaagaa gtactccatt
     6061 gggctcgcta tcggcacaaa cagcgtcggc tgggccgtca ttacggacga gtacaaggtg
     6121 ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa gaagaacctc
     6181 attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct caaaagaaca
     6241 gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga gatctttagt
     6301 aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc ctttttggtg
     6361 gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga cgaggtggcg
     6421 taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga cagtactgat
     6481 aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt tcggggacac
     6541 ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact ctttatccaa
     6601 ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc cggagttgac
     6661 gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa cctcatcgca
     6721 cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct gtcactcggg
     6781 ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct tcaactgagc
     6841 aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga ccagtacgca
     6901 gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctgagtga tattctgcga
     6961 gtgaacacgg agatcaccaa agctccgctg agcgctagta tgatcaagcg ctatgatgag
     7021 caccaccaag acttgacttt gctgaaggcc cttgtcagac agcaactgcc tgagaagtac
     7081 aaggaaattt tcttcgatca gtctaaaaat ggctacgccg gatacattga cggcggagca
     7141 agccaggagg aattttacaa atttattaag cccatcttgg aaaaaatgga cggcaccgag
     7201 gagctgctgg taaagcttaa cagagaagat ctgttgcgca aacagcgcac tttcgacaat
     7261 ggaagcatcc cccaccagat tcacctgggc gaactgcacg ctatcctcag gcggcaagag
     7321 gatttctacc cctttttgaa agataacagg gaaaagattg agaaaatcct cacatttcgg
     7381 ataccctact atgtaggccc cctcgcccgg ggaaattcca gattcgcgtg gatgactcgc
     7441 aaatcagaag agaccatcac tccctggaac ttcgaggaag tcgtggataa gggggcctct
     7501 gcccagtcct tcatcgaaag gatgactaac tttgataaaa atctgcctaa cgaaaaggtg
     7561 cttcctaaac actctctgct gtacgagtac ttcacagttt ataacgagct caccaaggtc
     7621 aaatacgtca cagaagggat gagaaagcca gcattcctgt ctggagagca gaagaaagct
     7681 atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg tgaaacagct caaagaagac
     7741 tatttcaaaa agattgaatg tttcgactct gttgaaatca gcggagtgga ggatcgcttc
     7801 aacgcatccc tgggaacgta tcacgatctc ctgaaaatca ttaaagacaa ggacttcctg
     7861 gacaatgagg agaacgagga cattcttgag gacattgtcc tcacccttac gttgtttgaa
     7921 gatagggaga tgattgaaga acgcttgaaa acttacgctc atctcttcga cgacaaagtc
     7981 atgaaacagc tcaagaggcg ccgatataca ggatgggggc ggctgtcaag aaaactgatc
     8041 aatgggatcc gagacaagca gagtggaaag acaatcctgg attttcttaa gtccgatgga
     8101 tttgccaacc ggaacttcat gcagttgatc catgatgact ctctcacctt taaggaggac
     8161 atccagaaag cacaagtttc tggccagggg gacagtcttc acgagcacat cgctaatctt
     8221 gcaggtagcc cagctatcaa aaagggaata ctgcagaccg ttaaggtcgt ggatgaactc
     8281 gtcaaagtaa tgggaaggca taagcccgag aatatcgtta tcgagatggc ccgagagaac
     8341 caaactaccc agaagggaca gaagaacagt agggaaagga tgaagaggat tgaagagggt
     8401 ataaaagaac tggggtccca aatccttaag gaacacccag ttgaaaacac ccagcttcag
     8461 aatgagaagc tctacctgta ctacctgcag aacggcaggg acatgtacgt ggatcaggaa
     8521 ctggacatca atcggctctc cgactacgac gtggatgcta tcgtgcccca gtcttttctc
     8581 aaagatgatt ctattgataa taaagtgttg acaagatccg ataaaaatag agggaagagt
     8641 gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa attattggcg gcagctgctg
     8701 aacgccaaac tgatcacaca acggaagttc gataatctga ctaaggctga acgaggtggc
     8761 ctgtctgagt tggataaagc cggcttcatc aaaaggcagc ttgttgagac acgccagatc
     8821 accaagcacg tggcccaaat tctcgattca cgcatgaaca ccaagtacga tgaaaatgac
     8881 aaactgattc gagaggtgaa agttattact ctgaagtcta agctggtctc agatttcaga
     8941 aaggactttc agttttataa ggtgagagag atcaacaatt accaccatgc gcatgatgcc
     9001 tacctgaatg cagtggtagg cactgcactt atcaaaaaat atcccaagct tgaatctgaa
     9061 tttgtttacg gagactataa agtgtacgat gttaggaaaa tgatcgcaaa gtctgagcag
     9121 gaaataggca aggccaccgc taagtacttc ttttacagca atattatgaa ttttttcaag
     9181 accgagatta cactggccaa tggagagatt cggaagcgac cacttatcga aacaaacgga
     9241 gaaacaggag aaatcgtgtg ggacaagggt agggatttcg cgacagtccg gaaggtcctg
     9301 tccatgccgc aggtgaacat cgttaaaaag accgaagtac agaccggagg cttctccaag
     9361 gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg cacgcaaaaa agattgggac
     9421 cccaagaaat acggcggatt cgattctcct acagtcgctt acagtgtact ggttgtggcc
     9481 aaagtggaga aagggaagtc taaaaaactc aaaagcgtca aggaactgct gggcatcaca
     9541 atcatggagc gatcaagctt cgaaaaaaac cccatcgact ttctcgaggc gaaaggatat
     9601 aaagaggtca aaaaagacct catcattaag cttcccaagt actctctctt tgagcttgaa
     9661 aacggccgga aacgaatgct cgctagtgcg ggcgagctgc agaaaggtaa cgagctggca
     9721 ctgccctcta aatacgttaa tttcttgtat ctggccagcc actatgaaaa gctcaaaggg
     9781 tctcccgaag ataatgagca gaagcagctg ttcgtggaac aacacaaaca ctaccttgat
     9841 gagatcatcg agcaaataag cgaattctcc aaaagagtga tcctcgccga cgctaacctc
     9901 gataaggtgc tttctgctta caataagcac agggataagc ccatcaggga gcaggcagaa
     9961 aacattatcc acttgtttac tctgaccaac ttgggcgcgc ctgcagcctt caagtacttc
    10021 gacaccacca tagacagaaa gcggtacacc tctacaaagg aggtcctgga cgccacactg
    10081 attcatcagt caattacggg gctctatgaa acaagaatcg acctctctca gctcggtgga
    10141 gacagcaggg ctgaccccaa gaagaagagg aaggtggagg ccagcggttc cggacgggct
    10201 gacgcattgg acgattttga tctggatatg ctgggaagtg acgccctcga tgattttgac
    10261 cttgacatgc ttggttcgga tgcccttgat gactttgacc tcgacatgct cggcagtgac
    10321 gcccttgatg atttcgacct ggacatgctg attaactcta gaagttccgg atctccgaaa
    10381 aagaaacgca aagttggtag ccagtacctg cccgacaccg acgaccggca ccggatcgag
    10441 gaaaagcgga agcggaccta cgagacattc aagagcatca tgaagaagtc ccccttcagc
    10501 ggccccaccg accctagacc tccacctaga agaatcgccg tgcccagcag atccagcgcc
    10561 agcgtgccaa aacctgcccc ccagccttac cccttcacca gcagcctgag caccatcaac
    10621 tacgacgagt tccctaccat ggtgttcccc agcggccaga tctctcaggc ctctgctctg
    10681 gctccagccc ctcctcaggt gctgcctcag gctcctgctc ctgcaccagc tccagccatg
    10741 gtgtctgcac tggctcaggc accagcaccc gtgcctgtgc tggctcctgg acctccacag
    10801 gctgtggctc caccagcccc taaacctaca caggccggcg agggcacact gtctgaagct
    10861 ctgctgcagc tgcagttcga cgacgaggat ctgggagccc tgctgggaaa cagcaccgat
    10921 cctgccgtgt tcaccgacct ggccagcgtg gacaacagcg agttccagca gctgctgaac
    10981 cagggcatcc ctgtggcccc tcacaccacc gagcccatgc tgatggaata ccccgaggcc
    11041 atcacccggc tcgtgacagg cgctcagagg cctcctgatc cagctcctgc ccctctggga
    11101 gcaccaggcc tgcctaatgg actgctgtct ggcgacgagg acttcagctc tatcgccgac
    11161 atggacttct ccgcactgct gggtagcgga tcgggatctc gggattccag ggaagggatg
    11221 tttttgccga agcctgaggc cggctccgct attagtgacg tgtttgaggg ccgcgaggtg
    11281 tgccagccaa aacgaatccg gccatttcat cctccaggaa gtccatgggc caaccgccca
    11341 ctccccgcca gcctcgcacc aacaccaacc ggtccagtac atgagccagt cgggtcactg
    11401 accccggcac cagtccctca gccactggat ccagcgcccg cagtgactcc cgaggccagt
    11461 cacctgttgg aggatcccga tgaagagacg agccaggctg tcaaagccct tcgggagatg
    11521 gccgatactg tgattcccca gaaggaagag gctgcaatct gtggccaaat ggacctttcc
    11581 catccgcccc caaggggcca tctggatgag ctgacaacca cacttgagtc catgaccgag
    11641 gatctgaacc tggactcacc cctgaccccg gaattgaacg agattctgga taccttcctg
    11701 aacgacgagt gcctcttgca tgccatgcat atcagcacag gactgtccat cttcgacaca
    11761 tctctgtttt gaaacccagc tttcttgtac aaagtggtga tgggctgcag gaattcgata
    11821 tcaagcttat cgataccgtc gacctcgagt catgtaatta gttatgtcac gcttacattc
    11881 acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta
    11941 ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat
    12001 ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct
    12061 tgagaaggtt ttgggacgct cgaaggcttt aatttgcggc cggtacccaa ttcgccctat
    12121 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
    12181 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
    12241 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
    12301 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
    12361 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    12421 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    12481 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
    12541 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
    12601 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
    12661 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
    12721 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
    12781 gcatctgtgc ggtatttcac accgcatagg gtaataactg atataattaa attgaagctc
    12841 taatttgtga gtttagtata catgcattta cttataatac agttttttag ttttgctggc
    12901 cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag
    12961 catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga
    13021 ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca
    13081 caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag
    13141 caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat
    13201 attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag
    13261 gttcctttgt tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca
    13321 caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact
    13381 gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt
    13441 acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga
    13501 tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta
    13561 attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga
    13621 tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag
    13681 ctttcgacat gatttatctt cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga
    13741 atactgggca atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc
    13801 tgtgctcctt ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaag
    13861 gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga attgaaaagg tggtatggtg
    13921 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
    13981 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
    14041 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
    14101 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
    14161 ttagacggat cgcttgcctg taacttacac gcgcctcgta tcttttaatg atggaataat
    14221 ttgggaattt actctgtgtt tatttatttt tatgttttgt atttggattt tagaaagtaa
    14281 ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg tttaaaaaat
    14341 ttcaacaaaa agc
//
