LOCUS       sequence-201379-       13448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To insert a single copy of a construct at a site on Chr II, 
            corresponding to GFP-labelled RAB-11 expressed specifically in the 
            epidermis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13448)
  AUTHORS   Taffoni C, Omi S, Huber C, Mailfert S, Fallet M, Rupprecht JF, 
            Ewbank JJ, Pujol N
  TITLE     Microtubule plus-end dynamics link wound repair to the innate immune
            response.
  JOURNAL   Elife. 2020 Jan 29;9. pii: 45047. doi: 10.7554/eLife.45047.
  PUBMED    31995031
REFERENCE   2  (bases 1 to 13448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-01-29"; volume: "9. pii: 45047. doi"; pages: " 
            10.7554/eLife.45047"
COMMENT     Sequence Label: pNP159
FEATURES             Location/Qualifiers
     source          1..13448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1338..1371)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1371..1382)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2804..3127)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5045..5069
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5072..6097
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5072..6097
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6098..6122)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6475..6508)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     6877..6894
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6878..6894
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7938..7966)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     8714..8734
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    8775..8799
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9583..9607)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11309..11328)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11428..11450
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11488..11506)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11574..11678
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11679..11747,11748..12539)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11897..11916)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12710..13298
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13199..13218
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtcggatt atgggctctt cttttatcct ctactttctt tctagaaaat gcaaatgttg
       61 agtcagaaat tgctgaatgg agtacaactg caatgggttt agcttatgtt tctggaacaa
      121 ttactggtgg aattgtgatt gaaaagtaag gttatatttc atttcaataa atgttaccac
      181 agtataacaa aaacaagtta cattgttcag agtatgtctg aatgtattat tattattgta
      241 tttgtgcatc agtactgtag ttgtactgaa gaagggttta aaataatctc aacgttttta
      301 agacgagtta gtgagaatac ggtcgcagtt aggatgtttg gacatagttt tattagataa
      361 tcgtgaacta actcactata aaaacccgct aactcattga ataagatagt tttaaacgtt
      421 caaaatttga agacaaaatt gtatgaataa gtttacagag ttggtcgacg caagcttctc
      481 cttctcttca catttctcaa taatttagct ctaattgcat tcgtattctt cgcaaaaata
      541 cgtattctga ttgacccgat gaaatacgga tgcctagttg cattgatcat ctacggttat
      601 acttatgggt gaggtttttc aaatatattt tgatttaatc gaataatgaa atttcagaac
      661 tggagttggt ccaatttcgt ggtttatatc ctctgaactg gtacctcaaa aacatcgtag
      721 tattgctcaa tctgtggcat attcaatgaa tactgtaatg gtggttatca gtacatttac
      781 agtattacct ctatattccg ttattggtag ttatgctttc cttattctat attcaattcc
      841 ttcatttata agtatgctta tattatttcg ctacttaccg gaaaccaaag gacgagagat
      901 tcatgatatt gtgaatgaac ttaaaaaaaa atgaaaacct ttgaaaacgt tcatacggcc
      961 gaaattttac ttgatcgagc caattgaact tttgttaaga cttacagaaa agtgagagat
     1021 tttgttacaa aaaagtgaga aaaaagcgaa aatgaacagg aaaaagaaga aaaatacacc
     1081 cgaagtatca tataaaaatt aaaaagattg tttgacctgg cggaactttt ttgcaaaaac
     1141 tctgaatatc catggcacat gcttcgtgca aaacaggatc caaatgaata tcaacaagag
     1201 attcttgaag acgacgagcc acttgctcag agcattgctt atctcgaatg agacccttgt
     1261 taaaatcatt tttcaaacat tatatgacat tgtcgaaatg tcctcctgat tccatgatgg
     1321 tagcaaactc acttcgtata acttcgtata gtatacctta tacgaagtta tctgccctcg
     1381 attgtctatc ttccattctc accaagtttc aacaacttta ttcagtcatt cttctctggt
     1441 tcatttccca accaatcttc ccgaataaag cccatctgtc ctttcggcca cattcctcac
     1501 ctacatccgt actccttatc tcccgttctt atccagtctt ttatcgtctc tgcgtctcgc
     1561 acactgtctc gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact tgcggaaaga
     1621 tttcctaata aaaggagacg aatccggtag gaaatcgaca agtctctgct tctcgaagac
     1681 taggtgagtt acatttcttt ggccgaatta ttttgaaatc ctaaatttga taatttcaga
     1741 gatgtctgta aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca ctcttatggt
     1801 atgcttcatg accatgtcaa ccatctactc ggaggttgat ggattcagag agaagcttga
     1861 taccgagatg aatgtgttca gagtgagtta aattatattt tgaattttaa ataattttta
     1921 attttctagc aatctaccaa tggattgtgg aaggacatag ttgtcatcgg aagatctagc
     1981 aagcgtgtcc gttgtcaata tgaagagacc aacgctaccc caactccaca tgctgatgga
     2041 tccccatctg ctccaccagg tcaaccacca gcagttccac cagtcttcaa ccagccaaag
     2101 actccaaatg gagccaatgg aaatggacca acctgcaact gcaatgctga taacaagtgc
     2161 ccagctggac catccggacc aaagggagtt ccaggagttc caggactcga cggagttcca
     2221 ggacttgacg gtgttccagg agttggagct gatgatatcg ctccacaacg cgagtctgtc
     2281 ggatgcttca cttgcccaca aggaccagtt ggaccaccag gagctcttgg aagaccagga
     2341 ccacgtggac ttccaggacc aagaggacaa aatggaaacc caggaagaga tggacaacca
     2401 ggacatccag gagagcaagg atcatccggt caaatcggaa agatcggaga gccaggacca
     2461 ccaggagaga agggacgcga cgccgagcat ccaatcggaa gaccaggacc aaagggacca
     2521 agaggagatc aaggaccaac aggaccagct ggacagaacg gtcttcacgg accaccagga
     2581 gagccaggaa ccgttggacc agaaggacca tctggaaagc aaggacgtca aggaccagac
     2641 ggaacccagg gagagactgg accagacgga agaccaggaa aggatgccga gtactgccag
     2701 tgcccagaca agtctccacc atcagaggct gtcaacgcca accgtggata cagaaatatc
     2761 taaattgttg gtgttttcta ataaaatatt tgagattcag tgagactttt ttcttggcgg
     2821 cacaataaag tttcctcttt ttgttgggtc actcaacgaa cacaatacaa atatttgagc
     2881 gctcgcattt ttctcaacct ttcgggttta aagcacttca aaatggaatt gaagagagat
     2941 tgtgaaagta catcagacag aatcacattt tgatagcatt cacttcactc agatgcaaac
     3001 ttttcagtga ctataaaaag tcataaaata ataacattaa aaaagcaaat aaattagcga
     3061 gagaattttt tgacaaaaag aaagaagagt gatagagaag aagggaatgc ttgaaaggat
     3121 tttgcattta tcttaatcac cgtcttcgag aagacgaacc atggcgccag tttcagaatc
     3181 caagtttcta atatagttca tcacaatgtt gacgttggtc caacctccgg cttgcataat
     3241 ctctgggatg gaaactccag cgcgagccat gtcacgggca gctccgacac gggcggaatg
     3301 tccagaccaa gccaagtaac gttgtccgga atcatccttg gctccgtaga tgagacggtg
     3361 ggtagcttcg aagattcctg aaaatttatt ttcagcttat tcttagctaa tatgttgttt
     3421 aaacttacct tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg cagcaactcc
     3481 gttcttgcga acacgacaga acaggtagtt gtttggatca tcggcgactc cggaaaccga
     3541 aatccagcgc tcaacgagct tggtaactcc aagggagagc gctttttcga ctccggcagt
     3601 cgagaccagc gtttttgtac gtccgatgtg gatgagcatt cttcctccgt ctgtgcgact
     3661 aatatccttg acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt tgtaagcaat
     3721 tccgaggaag gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca taagagaacg
     3781 gacctgatcg aagtcggttc tgaaaatttc gtcaagtctt aggattgaat acattaatat
     3841 ttaaacttac ctttcgaagg cgagggcttg cttggctctc tctccagcat caacattttc
     3901 cttgcggata cgtctcatta cgagagatac ggcattagaa tctgatgggc gtggaagtcc
     3961 agatcggcga tggagcatat tgagctgtcc gagatgttgt tggatcgtct tcaccgccaa
     4021 tccacgagct tggaggtaga gcaagtagtc acgaacgtct tctggctcgg ctgggaacca
     4081 cttgcggttg ttaagcttgc accaggcagc ccaagaacgg cacactgaga gaagcatctt
     4141 ccaggtgtgc tcggagaagg cttgtctgtc acggaacata tccatgaggt tcttgcgaac
     4201 ttcatcactt gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag tgagaagatt
     4261 tgacatattt tcgaagtttt ttagatgcac tagaacaaag cgtgttggct tcctctgagc
     4321 ccgctttcct tatatacccg cattctgcag ccttacagaa tgttctagaa ggtcctagat
     4381 gcattcgttt gaaaatactc ccggtgggtg caaagagacg cagacggaaa atgtatctgg
     4441 gtctctttat tgtgtacact acttttccat gtaccgaatg tgagtcgccc tccttttgca
     4501 acaagcagct cgaatgttct agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa
     4561 ataccgaaca acatttgctc taattgtgaa attagaaatc ttcaaactat aatcatgtca
     4621 ctttaccact atttccgtcc agctcaacgt tccgtttttg gtgatcattt ttgctttcgt
     4681 cgtaaatcta cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc aaattcgttg
     4741 actgagtatt caacgtttat acgttgtcgg caacgagaaa taggaaaatg catcgggaaa
     4801 tgttcttttt tcgatttttt ccaaggtttt gacaaatttt accacgaatt ttgctatgtt
     4861 ttcaattaaa aaatatgtta ttcaactgtt tctatgagga aaataaggct ttgcatgtaa
     4921 ttttcttatt cagcataatt tttaattaat ttgaattttc tgtcctaacg tttattttgt
     4981 tttcttggtt atgactgatc tgaaattaat ttttgaattt taaggtaata tgtcaggcgg
     5041 tgccgcaagt ttgtacaaaa aagcaggctc catgaaaaag cctgaactca ccgcgacgtc
     5101 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     5161 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     5221 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     5281 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     5341 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     5401 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     5461 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     5521 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     5581 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     5641 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     5701 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     5761 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     5821 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     5881 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     5941 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     6001 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     6061 ccgacgcccc agcactcgtc cgagggcaaa ggaatagacc cagctttctt gtacaaagtg
     6121 ggtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt
     6181 tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc
     6241 tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa
     6301 aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca caatgttctg
     6361 tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc
     6421 gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt tttaataact
     6481 tcgtatagta taccttatac gaagttatac tagtatatat ttttatgttt tcttttaaac
     6541 cttcctccat atttctgaat tgaatgttgt tgcatattgt gtgtccttat ttcatttcca
     6601 tcatcctttt gtgtgtttcc cccttccacc aattcccccc acgtcctaat gaaatgaaag
     6661 cgagaagata aataaacttg cacattgtgt tcagatgaga ggagcggaat acgaattggg
     6721 agacggaaag agaaaaaaaa atgtttagat ttggattgaa ttaatttttt agaaagcaag
     6781 aaaaaaaaca caaagagaat aaataaataa taggaaaaaa aacaaatatt cagtatttaa
     6841 aatttgaaaa ttcaacgacg ttggcgtcga tcatcctgta aaacgacggc cagtgccttg
     6901 aagccatatg gtcttgctgg aaactactgg gaaaagtttg gagaagtgat gattgatgtg
     6961 gtactggcgc aagaggcagt gcgagatctt ccgggagctg gacaggcttg ggtaattttt
     7021 accgcttgct tggtggatca gatgagagct ggatttgatg agaacagaaa aactgaccat
     7081 gaagctcaaa tgaaaaaaaa cacggttttg caccacgcta cgcagcagct attggaagac
     7141 gttagttcga atgtttcaaa aagttttaga gatgtttagt ttaagaatgt ggcatccacc
     7201 tcagcaacaa ctgcatcgtc tggaaatggt gagtgttcga gctccaacca accatccacg
     7261 agctccaatc aaccgttggc atgcccattt gcaagaatgt ctctgacgcc tgtgagttct
     7321 aataggattt ttgcattcct agttgataaa tgttcttatc caacgttttg caactgtaca
     7381 catttcagaa ggacgctcgt gtgatcgaaa gtctctccgg tagcggcggc agcggcagca
     7441 gcggtaacgg agacatcagt gacctctcgg gaacaatcta tttgtaatct cattccacga
     7501 tttctcgcaa cacatccctt atattctaca ttcctttttc gcatgtactc atctcttctc
     7561 gtttggaatc ttttcttatc tccgataatt gggttattgt gtcgataaag actgacctat
     7621 ttaacagata cactccacct tcatccacct ttccgttttc tacaatccat gttcttttca
     7681 attcaattca tttatcacac cctgttcttt tttttaattt atattttttg tcaaaactcg
     7741 aaatattttt tctaccgctc tgtgaaaaca aaacaagctt gcatgcctgc aggtcgactc
     7801 tagaggatcc ccgggattgg ccaaaggacc caaaggtatg tttcgaatga tactaacata
     7861 acatagaaca ttttcaggag gacccttgga gggtaccggt agaaaaaatg agtaaaggag
     7921 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
     7981 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     8041 aatttatttg cactactgga aaactacctg ttccatgggt aagtttaaac atatatatac
     8101 taactaaccc tgattattta aattttcagc caacacttgt cactactttc tgttatggtg
     8161 ttcaatgctt ctcgagatac ccagatcata tgaaacggca tgactttttc aagagtgcca
     8221 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
     8281 cacgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcaggtgctg
     8341 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
     8401 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
     8461 acatcatggc agacaaacaa aagaatggaa tcaaagttgt aagtttaaac atgattttac
     8521 taactaacta atctgattta aattttcaga acttcaaaat tagacacaac attgaagatg
     8581 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
     8641 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
     8701 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
     8761 atgaactata caaagcaagt ttgtacaaaa aagcaggctt gatgggctct cgtgacgatg
     8821 aatacgacta cttgttcaag ggtaattttg tttttcattt ttcaattccc aaacgaaaaa
     8881 ttgtttcagt tgttctgatt ggagactcag gcgtcggaaa gtcgaatctc ctgtctcgtt
     8941 tcacaagaaa tgagttcaac ttggaatcaa aatcaacaat cggagtcgag tttgccacga
     9001 gaagcatctc ggtgaggaac ttcattagaa tacacttatg attcaaatta ttatttttgt
     9061 aggtagaagg caagacagtg aaggctcaaa tttgggatac tgctggacag gaacgttacc
     9121 gtgccatcac atccgcttac tatcgtgggg ctgtcggagc tctcctagtc tacgacatcg
     9181 ctaagcatgt gacgtacgag aatgttgagc gatggttgaa ggagcttcgt gatcacgccg
     9241 atcagaacat tgtgattatg ttggtcggaa acaagagcga cttgcgccat ttgcgtgcag
     9301 ttccaacaga cgaggccaag atctacgccg aaagaaatca attgtcgttt attggtaagt
     9361 taaactaatc tattgcgttt atatattttt ttttttcaga aacatctgcc ctcgacagca
     9421 ccaacgttga agcagctttc actaatatcc tgacggaaat ctacaaatca gtatccaaca
     9481 agcatgtagg aactgacaga caaggatatg gcggtggcag tggtacaatc attccttcgc
     9541 cagcgtccga cccaccaaag aagcagtgtt gcatcccata aaacccagct ttcttgtaca
     9601 aagtggacca ttcgtagaat tccaactgag cgccggtcgc taccattacc aacttgtctg
     9661 gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt taaactgagt tctactaact
     9721 aacgagtaat atttaaattt tcagcatctc gcgcccgtgc ctctgacttc taagtccaat
     9781 tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat
     9841 tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca cctctaacaa
     9901 tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa aaaaattgtg
     9961 ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc tgtgtacact
    10021 tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa
    10081 aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc
    10141 tgggcctcta tgcgcgatgc cgtatcacaa ctgagaaaaa agacaaaact tattgaacag
    10201 tgttcctttc aaaaaattat tttgtaaaaa atcgagtttt tctccaaaat aacgcacgca
    10261 atcaaaaaca agaaaactaa cgtgttctcc tgggcgtgga actccttatc aagattacca
    10321 ttattgtctg tcagaattga tatttcaatt atttcatatc tagacaaacc cgggtgtctt
    10381 gcatttatta gtactgtgag tttacataca aacgaggctt actcagataa catagagtca
    10441 cctctaatta gcaaaaaccc atttctaatt agctatttcg accagttcta aagtcaccgt
    10501 aattagaaat ggtttttgta gcttttctaa ttagaaatga ccccgagata agtgaaatat
    10561 ggcattttct gggttttcta gtaagaaatg atcccgcacg tagtgattcc gcgttacaag
    10621 ccaaattcaa attaaaaaag tatattacac aattcaagtt acattatttt caggaaacaa
    10681 tggcgaagag caaagcagag attcaaaagg aggaaagaga caacagatat gaacgaagac
    10741 ttcttgaaaa gagaaagaga catgaggtga gtaaccatga taataaataa tcaagtaagg
    10801 gctatagatt tataaattct ggagttaaca gtttcgaaaa aaagtagttt tcatatgtgc
    10861 gaaggaatat caaaaatagg ggaatagcct aaacgagtga gaaaattaaa tgtttgttat
    10921 tttttcaaaa atgtctgcag ggccgttatc ctttcaaatg cactctatca aacatagatc
    10981 atatccatca tatcttatcc agaatttata tcatatccaa gattgagagc gtttcaagga
    11041 aaatctcaaa caaccataca attttgtaga tcgacaacaa ggcctccact tcttccacgt
    11101 gacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    11161 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    11221 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
    11281 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
    11341 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
    11401 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
    11461 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
    11521 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
    11581 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    11641 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
    11701 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
    11761 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
    11821 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    11881 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
    11941 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    12001 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    12061 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    12121 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    12181 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    12241 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    12301 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    12361 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    12421 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    12481 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    12541 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    12601 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    12661 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    12721 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    12781 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    12841 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    12901 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    12961 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    13021 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    13081 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    13141 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    13201 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    13261 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    13321 tacggttcct ggtcttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    13381 attctgtgga taaccgtatt accgcctttg agtgagctga taccagcgga taacaatttc
    13441 acagggcc
//
