LOCUS       sequence-201381-       13311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To insert a single copy of a construct at a site on Chr II, 
            corresponding to mKate expressed specifically in the epidermis; can 
            be used for N- or C-terminal fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13311)
  TITLE     Ewbank lab plasmids
REFERENCE   2  (bases 1 to 13311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pNP172
FEATURES             Location/Qualifiers
     source          1..13311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1338..1371)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1371..1382)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2804..3127)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5045..5069
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5072..6097
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5072..6097
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6098..6122)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6475..6508)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     6877..6894
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6878..6894
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9589..9669
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      9678..10001
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(11172..11191)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11291..11313
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11351..11369)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11437..11541
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11542..11610,11611..12402)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11760..11779)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12573..13161
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13062..13081
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtcggatt atgggctctt cttttatcct ctactttctt tctagaaaat gcaaatgttg
       61 agtcagaaat tgctgaatgg agtacaactg caatgggttt agcttatgtt tctggaacaa
      121 ttactggtgg aattgtgatt gaaaagtaag gttatatttc atttcaataa atgttaccac
      181 agtataacaa aaacaagtta cattgttcag agtatgtctg aatgtattat tattattgta
      241 tttgtgcatc agtactgtag ttgtactgaa gaagggttta aaataatctc aacgttttta
      301 agacgagtta gtgagaatac ggtcgcagtt aggatgtttg gacatagttt tattagataa
      361 tcgtgaacta actcactata aaaacccgct aactcattga ataagatagt tttaaacgtt
      421 caaaatttga agacaaaatt gtatgaataa gtttacagag ttggtcgacg caagcttctc
      481 cttctcttca catttctcaa taatttagct ctaattgcat tcgtattctt cgcaaaaata
      541 cgtattctga ttgacccgat gaaatacgga tgcctagttg cattgatcat ctacggttat
      601 acttatgggt gaggtttttc aaatatattt tgatttaatc gaataatgaa atttcagaac
      661 tggagttggt ccaatttcgt ggtttatatc ctctgaactg gtacctcaaa aacatcgtag
      721 tattgctcaa tctgtggcat attcaatgaa tactgtaatg gtggttatca gtacatttac
      781 agtattacct ctatattccg ttattggtag ttatgctttc cttattctat attcaattcc
      841 ttcatttata agtatgctta tattatttcg ctacttaccg gaaaccaaag gacgagagat
      901 tcatgatatt gtgaatgaac ttaaaaaaaa atgaaaacct ttgaaaacgt tcatacggcc
      961 gaaattttac ttgatcgagc caattgaact tttgttaaga cttacagaaa agtgagagat
     1021 tttgttacaa aaaagtgaga aaaaagcgaa aatgaacagg aaaaagaaga aaaatacacc
     1081 cgaagtatca tataaaaatt aaaaagattg tttgacctgg cggaactttt ttgcaaaaac
     1141 tctgaatatc catggcacat gcttcgtgca aaacaggatc caaatgaata tcaacaagag
     1201 attcttgaag acgacgagcc acttgctcag agcattgctt atctcgaatg agacccttgt
     1261 taaaatcatt tttcaaacat tatatgacat tgtcgaaatg tcctcctgat tccatgatgg
     1321 tagcaaactc acttcgtata acttcgtata gtatacctta tacgaagtta tctgccctcg
     1381 attgtctatc ttccattctc accaagtttc aacaacttta ttcagtcatt cttctctggt
     1441 tcatttccca accaatcttc ccgaataaag cccatctgtc ctttcggcca cattcctcac
     1501 ctacatccgt actccttatc tcccgttctt atccagtctt ttatcgtctc tgcgtctcgc
     1561 acactgtctc gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact tgcggaaaga
     1621 tttcctaata aaaggagacg aatccggtag gaaatcgaca agtctctgct tctcgaagac
     1681 taggtgagtt acatttcttt ggccgaatta ttttgaaatc ctaaatttga taatttcaga
     1741 gatgtctgta aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca ctcttatggt
     1801 atgcttcatg accatgtcaa ccatctactc ggaggttgat ggattcagag agaagcttga
     1861 taccgagatg aatgtgttca gagtgagtta aattatattt tgaattttaa ataattttta
     1921 attttctagc aatctaccaa tggattgtgg aaggacatag ttgtcatcgg aagatctagc
     1981 aagcgtgtcc gttgtcaata tgaagagacc aacgctaccc caactccaca tgctgatgga
     2041 tccccatctg ctccaccagg tcaaccacca gcagttccac cagtcttcaa ccagccaaag
     2101 actccaaatg gagccaatgg aaatggacca acctgcaact gcaatgctga taacaagtgc
     2161 ccagctggac catccggacc aaagggagtt ccaggagttc caggactcga cggagttcca
     2221 ggacttgacg gtgttccagg agttggagct gatgatatcg ctccacaacg cgagtctgtc
     2281 ggatgcttca cttgcccaca aggaccagtt ggaccaccag gagctcttgg aagaccagga
     2341 ccacgtggac ttccaggacc aagaggacaa aatggaaacc caggaagaga tggacaacca
     2401 ggacatccag gagagcaagg atcatccggt caaatcggaa agatcggaga gccaggacca
     2461 ccaggagaga agggacgcga cgccgagcat ccaatcggaa gaccaggacc aaagggacca
     2521 agaggagatc aaggaccaac aggaccagct ggacagaacg gtcttcacgg accaccagga
     2581 gagccaggaa ccgttggacc agaaggacca tctggaaagc aaggacgtca aggaccagac
     2641 ggaacccagg gagagactgg accagacgga agaccaggaa aggatgccga gtactgccag
     2701 tgcccagaca agtctccacc atcagaggct gtcaacgcca accgtggata cagaaatatc
     2761 taaattgttg gtgttttcta ataaaatatt tgagattcag tgagactttt ttcttggcgg
     2821 cacaataaag tttcctcttt ttgttgggtc actcaacgaa cacaatacaa atatttgagc
     2881 gctcgcattt ttctcaacct ttcgggttta aagcacttca aaatggaatt gaagagagat
     2941 tgtgaaagta catcagacag aatcacattt tgatagcatt cacttcactc agatgcaaac
     3001 ttttcagtga ctataaaaag tcataaaata ataacattaa aaaagcaaat aaattagcga
     3061 gagaattttt tgacaaaaag aaagaagagt gatagagaag aagggaatgc ttgaaaggat
     3121 tttgcattta tcttaatcac cgtcttcgag aagacgaacc atggcgccag tttcagaatc
     3181 caagtttcta atatagttca tcacaatgtt gacgttggtc caacctccgg cttgcataat
     3241 ctctgggatg gaaactccag cgcgagccat gtcacgggca gctccgacac gggcggaatg
     3301 tccagaccaa gccaagtaac gttgtccgga atcatccttg gctccgtaga tgagacggtg
     3361 ggtagcttcg aagattcctg aaaatttatt ttcagcttat tcttagctaa tatgttgttt
     3421 aaacttacct tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg cagcaactcc
     3481 gttcttgcga acacgacaga acaggtagtt gtttggatca tcggcgactc cggaaaccga
     3541 aatccagcgc tcaacgagct tggtaactcc aagggagagc gctttttcga ctccggcagt
     3601 cgagaccagc gtttttgtac gtccgatgtg gatgagcatt cttcctccgt ctgtgcgact
     3661 aatatccttg acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt tgtaagcaat
     3721 tccgaggaag gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca taagagaacg
     3781 gacctgatcg aagtcggttc tgaaaatttc gtcaagtctt aggattgaat acattaatat
     3841 ttaaacttac ctttcgaagg cgagggcttg cttggctctc tctccagcat caacattttc
     3901 cttgcggata cgtctcatta cgagagatac ggcattagaa tctgatgggc gtggaagtcc
     3961 agatcggcga tggagcatat tgagctgtcc gagatgttgt tggatcgtct tcaccgccaa
     4021 tccacgagct tggaggtaga gcaagtagtc acgaacgtct tctggctcgg ctgggaacca
     4081 cttgcggttg ttaagcttgc accaggcagc ccaagaacgg cacactgaga gaagcatctt
     4141 ccaggtgtgc tcggagaagg cttgtctgtc acggaacata tccatgaggt tcttgcgaac
     4201 ttcatcactt gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag tgagaagatt
     4261 tgacatattt tcgaagtttt ttagatgcac tagaacaaag cgtgttggct tcctctgagc
     4321 ccgctttcct tatatacccg cattctgcag ccttacagaa tgttctagaa ggtcctagat
     4381 gcattcgttt gaaaatactc ccggtgggtg caaagagacg cagacggaaa atgtatctgg
     4441 gtctctttat tgtgtacact acttttccat gtaccgaatg tgagtcgccc tccttttgca
     4501 acaagcagct cgaatgttct agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa
     4561 ataccgaaca acatttgctc taattgtgaa attagaaatc ttcaaactat aatcatgtca
     4621 ctttaccact atttccgtcc agctcaacgt tccgtttttg gtgatcattt ttgctttcgt
     4681 cgtaaatcta cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc aaattcgttg
     4741 actgagtatt caacgtttat acgttgtcgg caacgagaaa taggaaaatg catcgggaaa
     4801 tgttcttttt tcgatttttt ccaaggtttt gacaaatttt accacgaatt ttgctatgtt
     4861 ttcaattaaa aaatatgtta ttcaactgtt tctatgagga aaataaggct ttgcatgtaa
     4921 ttttcttatt cagcataatt tttaattaat ttgaattttc tgtcctaacg tttattttgt
     4981 tttcttggtt atgactgatc tgaaattaat ttttgaattt taaggtaata tgtcaggcgg
     5041 tgccgcaagt ttgtacaaaa aagcaggctc catgaaaaag cctgaactca ccgcgacgtc
     5101 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     5161 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     5221 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     5281 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     5341 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     5401 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     5461 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     5521 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     5581 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     5641 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     5701 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     5761 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     5821 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     5881 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     5941 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     6001 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     6061 ccgacgcccc agcactcgtc cgagggcaaa ggaatagacc cagctttctt gtacaaagtg
     6121 ggtccaatta ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt
     6181 tttgttatta tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc
     6241 tctaacaatg aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa
     6301 aaattgtgct ccctcccccc attaataata attctatccc aaaatctaca caatgttctg
     6361 tgtacacttc ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc
     6421 gcacacaaaa taccttatca tatgttacgt ttcagtttat gaccgcaatt tttaataact
     6481 tcgtatagta taccttatac gaagttatac tagtatatat ttttatgttt tcttttaaac
     6541 cttcctccat atttctgaat tgaatgttgt tgcatattgt gtgtccttat ttcatttcca
     6601 tcatcctttt gtgtgtttcc cccttccacc aattcccccc acgtcctaat gaaatgaaag
     6661 cgagaagata aataaacttg cacattgtgt tcagatgaga ggagcggaat acgaattggg
     6721 agacggaaag agaaaaaaaa atgtttagat ttggattgaa ttaatttttt agaaagcaag
     6781 aaaaaaaaca caaagagaat aaataaataa taggaaaaaa aacaaatatt cagtatttaa
     6841 aatttgaaaa ttcaacgacg ttggcgtcga tcatcctgta aaacgacggc cagtgctgca
     6901 ttttcagtat ttgctattga caaataaaat ttccaattat tattcactta aacttctcgt
     6961 ttacacaaaa tcaaaagcga tgtaattgtt cctgattagt tcactcaaaa cttttttttc
     7021 acctactgtt tatctccttc tgttcttttt ctttattccc tctaattcat ccattctgtt
     7081 ttaattcaca ctgcgcgccg caacaccatg tcgctgtaat atttagtcag tttttgagaa
     7141 aataggtata gagacagaga cgctgacaag acaacactag gtataaaagc ggaagtggtg
     7201 gctttaaaat cacttggctt ctaaagtcca gtgacaggta aggttctcgt tacttccgtc
     7261 tcgattacta agatttgatt actttttaga aaaatggtct ccgagctcat taaagaaaac
     7321 atgcatatga agctctacat ggagggaacc gtcaacaacc accacttcaa gtgcacctcc
     7381 gagggagagg gaaagccata cgagggaacc caaaccatgc gtatcaaggc cgtcgaggga
     7441 ggaccactcc cattcgcctt cgacatcctc gccacctcct tcatgtacgg atccaaggta
     7501 agtttaaaca tatatatact aactaaccct gattatttaa attttcagac cttcatcaac
     7561 cacacccaag gaatcccaga cttcttcaag caatccttcc cagagggatt cacctgggag
     7621 cgtgtcacca cctacgagga cggaggagtc ctcaccgcca cccaagacac ctccctccaa
     7681 gacggatgcc tcatctacaa cgtcaaggta agtttaaaca gttcggtact aactaaccat
     7741 acatatttaa attttcagat ccgtggagtc aacttcccat ccaacggacc agtcatgcaa
     7801 aagaagaccc tcggatggga ggcctccacc gagaccctct acccagccga cggaggactt
     7861 gagggacgtg ccgacatggc cctcaagctc gtcggaggag gacacctcat ctgcaacctc
     7921 aaggtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagaccacc
     7981 taccgttcca agaagccagc caagaacctc aagatgccag gagtctacta cgtcgaccgt
     8041 cgtcttgagc gtatcaagga ggccgacaag gagacctacg tcgagcaaca cgaggtcgcc
     8101 gtcgcccgtt actgcgacct cccatccaag ctcggacacc gtggtggagg aggatctgga
     8161 ggaggaggat ccatggtttc cgagttgatc aaggagaata tgcacatgaa actctacatg
     8221 gaaggaaccg tcaacaatca tcatttcaaa tgtacttcag aaggagaagg aaaaccatac
     8281 gagggaactc agaccatgag aatcaaggct gttgagggag gtaagtttaa aaaatacgtg
     8341 ttcataactt gatacgaata taaattttca ggaccacttc cattcgcgtt cgacattctt
     8401 gccacttcgt tcatgtatgg aagcaagact ttcattaatc acacacaagg gatcccggat
     8461 ttcttcaagc aatctttccc agaaggattc acatgggaga gagttaccac ttacgaggat
     8521 ggaggagttc ttaccgccac ccaagacaca tccctccaag gtaagtttaa aagattatag
     8581 caatgttcgt ctaaatataa taaattttca gatggatgtc tgatctacaa cgtcaagatc
     8641 agaggagtca atttcccatc aaatggacca gttatgcaga agaagacttt gggctgggag
     8701 gcttccaccg agacccttta cccagcagat ggaggtctcg agggccgcgc tgatatggct
     8761 ctcaaactcg tcggaggagg acacttgatc tgcaatttaa aaaccaccta ccggtcgaag
     8821 gtaagtttaa aatcaatttt taatagctta tataataata caaattttca gaagccagcc
     8881 aagaatctca aaatgccagg agtttactat gttgaccgtc gtcttgagcg cattaaagag
     8941 gccgataagg aaacgtacgt tgaacaacat gaagttgccg ttgctcgata ttgtgatctc
     9001 ccatccaagc tcggacatcg tgccggccac ggactccaag acgacccaga cctccaagcc
     9061 ctcctcaagg gatcccaact cctcaaggtc aagtcctcct cctggcgtcg tgagcgtttc
     9121 tacaagctcc aagaggactg caaggtaagt ttaaacatat atatactaac taaccctgat
     9181 tatttaaatt ttcagaccat ctggcaagag tcccgtaagg taagtttaaa cagttcggta
     9241 ctaactaacc atacatattt aaattttcag gtcatgcgtt ccccagagtc ccaactcttc
     9301 tccatcgagg acatccaaga ggtccgtatg ggacaccgta ccgagggact cgagaagttc
     9361 gcccgtgaca tcccagagga ccgttgcttc tccatcgtct tcaaggtaag tttaaacatg
     9421 attttactaa ctaactaatc tgatttaaat tttcaggacc aacgtaacac cctcgacctc
     9481 atcgccccat ccccagccga cgcccaacac tgggtccaag gactccgtaa gatcatccac
     9541 cactccggat ccatggacca acgtcaaaag tcaggagcta gcggagccta cccttacgac
     9601 gtgccagatt acgcctaccc ctacgacgta ccagactacg cctacccata cgacgtccca
     9661 gactacgctt aagataaatg caaaatcctt tcaagcattc ccttcttctc tatcactctt
     9721 ctttcttttt gtcaaaaaat tctctcgcta atttatttgc ttttttaatg ttattatttt
     9781 atgacttttt atagtcactg aaaagtttgc atctgagtga agtgaatgct atcaaaatgt
     9841 gattctgtct gatgtacttt cacaatctct cttcaattcc attttgaagt gctttaaacc
     9901 cgaaaggttg agaaaaatgc gagcgctcaa atatttgtat tgtgttcgtt gagtgaccca
     9961 acaaaaagag gaaactttat tgtgccgcca agaaaaaagt ctcacgtacg gtgcgcgcga
    10021 tgccgtatca caactgagaa aaaagacaaa acttattgaa cagtgttcct ttcaaaaaat
    10081 tattttgtaa aaaatcgagt ttttctccaa aataacgcac gcaatcaaaa acaagaaaac
    10141 taacgtgttc tcctgggcgt ggaactcctt atcaagatta ccattattgt ctgtcagaat
    10201 tgatatttca attatttcat atctagacaa acccgggtgt cttgcattta ttagtactgt
    10261 gagtttacat acaaacgagg cttactcaga taacatagag tcacctctaa ttagcaaaaa
    10321 cccatttcta attagctatt tcgaccagtt ctaaagtcac cgtaattaga aatggttttt
    10381 gtagcttttc taattagaaa tgaccccgag ataagtgaaa tatggcattt tctgggtttt
    10441 ctagtaagaa atgatcccgc acgtagtgat tccgcgttac aagccaaatt caaattaaaa
    10501 aagtatatta cacaattcaa gttacattat tttcaggaaa caatggcgaa gagcaaagca
    10561 gagattcaaa aggaggaaag agacaacaga tatgaacgaa gacttcttga aaagagaaag
    10621 agacatgagg tgagtaacca tgataataaa taatcaagta agggctatag atttataaat
    10681 tctggagtta acagtttcga aaaaaagtag ttttcatatg tgcgaaggaa tatcaaaaat
    10741 aggggaatag cctaaacgag tgagaaaatt aaatgtttgt tattttttca aaaatgtctg
    10801 cagggccgtt atcctttcaa atgcactcta tcaaacatag atcatatcca tcatatctta
    10861 tccagaattt atatcatatc caagattgag agcgtttcaa ggaaaatctc aaacaaccat
    10921 acaattttgt agatcgacaa caaggcctcc acttcttcca cgtgacgtcg tgactgggaa
    10981 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    11041 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    11101 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
    11161 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
    11221 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
    11281 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
    11341 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
    11401 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    11461 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    11521 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    11581 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    11641 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    11701 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    11761 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
    11821 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    11881 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    11941 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    12001 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    12061 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    12121 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    12181 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    12241 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    12301 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    12361 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    12421 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    12481 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    12541 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    12601 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    12661 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
    12721 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    12781 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    12841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    12901 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    12961 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    13021 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    13081 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    13141 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggtcttt
    13201 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    13261 attaccgcct ttgagtgagc tgataccagc ggataacaat ttcacagggc c
//
