LOCUS       sequence-201382-       13882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A vector backbone, including mKate, to be used to make constructs to
            be inserted as a single copy at a site on Chr I..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   Lee SH, Omi S, Thakur N, Taffoni C, Belougne J, Engelmann I, Ewbank 
            JJ, Pujol N
  TITLE     Modulatory upregulation of an insulin peptide gene by different 
            pathogens in C. elegans.
  JOURNAL   Virulence. 2018 Dec 31;9(1):648-658. doi: 
            10.1080/21505594.2018.1433969.
  PUBMED    29405821
REFERENCE   2  (bases 1 to 13882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1080/21505594.2018"; journalName: "Virulence"; date: 
            "2018-12-31- 31"; volume: "9"; issue: "1"; pages: "648-658"
COMMENT     Sequence Label: pSO5
FEATURES             Location/Qualifiers
     source          1..13882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1553..1586)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1586..1597)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(3019..3342)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5260..5284
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5287..6312
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5287..6312
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6313..6337)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6690..6723)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     7092..7109
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7093..7109
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7370..7675
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             9710..10015
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(11743..11762)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11862..11884
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11922..11940)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12008..12112
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12113..12181,12182..12973)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12331..12350)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13144..13732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13633..13652
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caagtgggga tcaggaagaa gttgataaag tgattcaaaa actcgaggag ctcaacacag
       61 cgatgaaccc aattttgaga gcaacactga cgaagaacta tgacgcgagg gtaggcacta
      121 ccttgaagct atccacctgc ctgcctgccg cctatttcca tgtgcagtag gaacgtgtgt
      181 aggtataggc aggcattatc gcgtctcctt tcgaactacc tagtacctag taggtagttt
      241 gaagggaggc gcgataatgc ctgcctatgt ctacacatgt gcctactgcg catggaaata
      301 ggcggtagtc aggcaggtag atagcctcat aaaattcaat acttcctttt cagaaagaat
      361 gctggaatgt attcaaagag gagtcactct tcccattgct cagagaatat gaagagttgc
      421 tcggcgagca aaagttcatg attggagatc gatattcatg ggctgacatc gctgtcgtcg
      481 agtttctaac tcggtgtcag cagtgctacg atagcttcta tctggctcat ttcgagaggc
      541 tccgaacttt ttgccagact tttgagtctc ttccgcacat tcgtccctac attcagggac
      601 gtgttgattc atttatctaa ttttttaaaa tggatatttt tcgtttttta tttgtttgtt
      661 ttgctgataa ttcaataaag ttacgcaacg aattttagac atttatgaaa accgaaaatc
      721 gacaaaaata ttttaagaaa aatagcagca aaattcaata ttttatcaaa ttttgtttat
      781 ggtaatgaga aagaaaacat acaatatttt tctaagaaga agaaaaatga ccagaatttt
      841 aagatttttc tcgaatatac gaaaaattga taaactaaaa ttttcgattt ttccatttat
      901 tttgaaaaaa aaactcttga cggttttcac catgcgaaga ggtgagaaat attctgaaag
      961 ttatgcattt tgaaaaaatt gcaaaaaaat acgttaaaat tttttgatct attgtttttt
     1021 tttgaaaacc ggaatttaaa atggaatttt ttttcaaaaa ttaaaattaa aatttaaata
     1081 tttctagtgt ttggatagtt tttcaaaagc tagatttaac ccgcgagatg tcgaccgcta
     1141 gtgtagctta cagatggctg aagacttcgc aaaaatctta tcttagaaac ccgcgaggtg
     1201 tcggccgcta aaactttgta aacctgccaa caaaatgctt cgcattgtct gaacagatga
     1261 aaccttgaaa caaaacatat acaattgaaa tcccgtaaag tgtcgggcgc aagaactgcg
     1321 attagttgta taattggact agctcaaatt tgaccaaaat ctcattttta gacctctaag
     1381 atgtcggcca ctataacttt attcaacttg aaactgcaaa aaaacttatc aactgtatag
     1441 attcaagtat tatgtcaagt attggtttta gagctacagt acactctgat aacaaaatct
     1501 gcagaaatac ctccctgtca attcccaaaa tacggtagca aactcacttc gtataacttc
     1561 gtatagtata ccttatacga agttatctgc cctcgattgt ctatcttcca ttctcaccaa
     1621 gtttcaacaa ctttattcag tcattcttct ctggttcatt tcccaaccaa tcttcccgaa
     1681 taaagcccat ctgtcctttc ggccacattc ctcacctaca tccgtactcc ttatctcccg
     1741 ttcttatcca gtcttttatc gtctctgcgt ctcgcacact gtctcgtcgg taatgtaaaa
     1801 aaaaaacaaa aaaaacttga aaacttgcgg aaagatttcc taataaaagg agacgaatcc
     1861 ggtaggaaat cgacaagtct ctgcttctcg aagactaggt gagttacatt tctttggccg
     1921 aattattttg aaatcctaaa tttgataatt tcagagatgt ctgtaaaact tgcgtgttat
     1981 gtgacggctt cggtcactgt cgccactctt atggtatgct tcatgaccat gtcaaccatc
     2041 tactcggagg ttgatggatt cagagagaag cttgataccg agatgaatgt gttcagagtg
     2101 agttaaatta tattttgaat tttaaataat ttttaatttt ctagcaatct accaatggat
     2161 tgtggaagga catagttgtc atcggaagat ctagcaagcg tgtccgttgt caatatgaag
     2221 agaccaacgc taccccaact ccacatgctg atggatcccc atctgctcca ccaggtcaac
     2281 caccagcagt tccaccagtc ttcaaccagc caaagactcc aaatggagcc aatggaaatg
     2341 gaccaacctg caactgcaat gctgataaca agtgcccagc tggaccatcc ggaccaaagg
     2401 gagttccagg agttccagga ctcgacggag ttccaggact tgacggtgtt ccaggagttg
     2461 gagctgatga tatcgctcca caacgcgagt ctgtcggatg cttcacttgc ccacaaggac
     2521 cagttggacc accaggagct cttggaagac caggaccacg tggacttcca ggaccaagag
     2581 gacaaaatgg aaacccagga agagatggac aaccaggaca tccaggagag caaggatcat
     2641 ccggtcaaat cggaaagatc ggagagccag gaccaccagg agagaaggga cgcgacgccg
     2701 agcatccaat cggaagacca ggaccaaagg gaccaagagg agatcaagga ccaacaggac
     2761 cagctggaca gaacggtctt cacggaccac caggagagcc aggaaccgtt ggaccagaag
     2821 gaccatctgg aaagcaagga cgtcaaggac cagacggaac ccagggagag actggaccag
     2881 acggaagacc aggaaaggat gccgagtact gccagtgccc agacaagtct ccaccatcag
     2941 aggctgtcaa cgccaaccgt ggatacagaa atatctaaat tgttggtgtt ttctaataaa
     3001 atatttgaga ttcagtgaga cttttttctt ggcggcacaa taaagtttcc tctttttgtt
     3061 gggtcactca acgaacacaa tacaaatatt tgagcgctcg catttttctc aacctttcgg
     3121 gtttaaagca cttcaaaatg gaattgaaga gagattgtga aagtacatca gacagaatca
     3181 cattttgata gcattcactt cactcagatg caaacttttc agtgactata aaaagtcata
     3241 aaataataac attaaaaaag caaataaatt agcgagagaa ttttttgaca aaaagaaaga
     3301 agagtgatag agaagaaggg aatgcttgaa aggattttgc atttatctta atcaccgtct
     3361 tcgagaagac gaaccatggc gccagtttca gaatccaagt ttctaatata gttcatcaca
     3421 atgttgacgt tggtccaacc tccggcttgc ataatctctg ggatggaaac tccagcgcga
     3481 gccatgtcac gggcagctcc gacacgggcg gaatgtccag accaagccaa gtaacgttgt
     3541 ccggaatcat ccttggctcc gtagatgaga cggtgggtag cttcgaagat tcctgaaaat
     3601 ttattttcag cttattctta gctaatatgt tgtttaaact taccttcaag agctcgagtc
     3661 gagagttgtg atgttgcact cggggcagca actccgttct tgcgaacacg acagaacagg
     3721 tagttgtttg gatcatcggc gactccggaa accgaaatcc agcgctcaac gagcttggta
     3781 actccaaggg agagcgcttt ttcgactccg gcagtcgaga ccagcgtttt tgtacgtccg
     3841 atgtggatga gcattcttcc tccgtctgtg cgactaatat ccttgacgcg aatgcgggca
     3901 atttcggcga tacggagaag tgtgttgtaa gcaattccga ggaaggcgag gtttcggatg
     3961 tcctggcaac gatcggaatt ttccataaga gaacggacct gatcgaagtc ggttctgaaa
     4021 atttcgtcaa gtcttaggat tgaatacatt aatatttaaa cttacctttc gaaggcgagg
     4081 gcttgcttgg ctctctctcc agcatcaaca ttttccttgc ggatacgtct cattacgaga
     4141 gatacggcat tagaatctga tgggcgtgga agtccagatc ggcgatggag catattgagc
     4201 tgtccgagat gttgttggat cgtcttcacc gccaatccac gagcttggag gtagagcaag
     4261 tagtcacgaa cgtcttctgg ctcggctggg aaccacttgc ggttgttaag cttgcaccag
     4321 gcagcccaag aacggcacac tgagagaagc atcttccagg tgtgctcgga gaaggcttgt
     4381 ctgtcacgga acatatccat gaggttcttg cgaacttcat cacttgtcgc gtcaactgga
     4441 agtgctggaa gattttgatg aacagtgaga agatttgaca tattttcgaa gttttttaga
     4501 tgcactagaa caaagcgtgt tggcttcctc tgagcccgct ttccttatat acccgcattc
     4561 tgcagcctta cagaatgttc tagaaggtcc tagatgcatt cgtttgaaaa tactcccggt
     4621 gggtgcaaag agacgcagac ggaaaatgta tctgggtctc tttattgtgt acactacttt
     4681 tccatgtacc gaatgtgagt cgccctcctt ttgcaacaag cagctcgaat gttctagaaa
     4741 aaggtggaaa atagtataaa taccgttgaa aataaatacc gaacaacatt tgctctaatt
     4801 gtgaaattag aaatcttcaa actataatca tgtcacttta ccactatttc cgtccagctc
     4861 aacgttccgt ttttggtgat catttttgct ttcgtcgtaa atctacacac gcgtctcttc
     4921 cgtgcgagag tccaagccag cagccaaatt cgttgactga gtattcaacg tttatacgtt
     4981 gtcggcaacg agaaatagga aaatgcatcg ggaaatgttc ttttttcgat tttttccaag
     5041 gttttgacaa attttaccac gaattttgct atgttttcaa ttaaaaaata tgttattcaa
     5101 ctgtttctat gaggaaaata aggctttgca tgtaattttc ttattcagca taatttttaa
     5161 ttaatttgaa ttttctgtcc taacgtttat tttgttttct tggttatgac tgatctgaaa
     5221 ttaatttttg aattttaagg taatatgtca ggcggtgccg caagtttgta caaaaaagca
     5281 ggctccatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     5341 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     5401 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     5461 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     5521 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     5581 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     5641 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     5701 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     5761 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     5821 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     5881 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     5941 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     6001 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     6061 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     6121 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     6181 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     6241 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     6301 gcaaaggaat agacccagct ttcttgtaca aagtgggtcc aattactctt caacatccct
     6361 acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct
     6421 taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc
     6481 aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa
     6541 taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt
     6601 ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt
     6661 tacgtttcag tttatgaccg caatttttaa taacttcgta tagtatacct tatacgaagt
     6721 tatactagta tatattttta tgttttcttt taaaccttcc tccatatttc tgaattgaat
     6781 gttgttgcat attgtgtgtc cttatttcat ttccatcatc cttttgtgtg tttccccctt
     6841 ccaccaattc cccccacgtc ctaatgaaat gaaagcgaga agataaataa acttgcacat
     6901 tgtgttcaga tgagaggagc ggaatacgaa ttgggagacg gaaagagaaa aaaaaatgtt
     6961 tagatttgga ttgaattaat tttttagaaa gcaagaaaaa aaacacaaag agaataaata
     7021 aataatagga aaaaaaacaa atattcagta tttaaaattt gaaaattcaa cgacgttggc
     7081 gtcgatcatc ctgtaaaacg acggccagtg cggccgctac ccgaagtatg tcaaaaagag
     7141 gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
     7201 ggcatatatg atgtcaatat ctccggttcg gtaagcacaa ccatgcagaa tgaagcccgt
     7261 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
     7321 tttattgaaa tgaacggctc ttttgccgac gagaacaggg gctggtgaaa tgcagtttaa
     7381 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
     7441 tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga
     7501 taaagtcccc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat
     7561 gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag
     7621 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
     7681 aggctccctt atacacagcc agtctgcagg tcgaatcgat ggtctccgag ctcattaaag
     7741 aaaacatgca tatgaagctc tacatggagg gaaccgtcaa caaccaccac ttcaagtgca
     7801 cctccgaggg agagggaaag ccatacgagg gaacccaaac catgcgtatc aaggccgtcg
     7861 agggaggacc actcccattc gccttcgaca tcctcgccac ctccttcatg tacggatcca
     7921 aggtaagttt aaacatatat atactaacta accctgatta tttaaatttt cagaccttca
     7981 tcaaccacac ccaaggaatc ccagacttct tcaagcaatc cttcccagag ggattcacct
     8041 gggagcgtgt caccacctac gaggacggag gagtcctcac cgccacccaa gacacctccc
     8101 tccaagacgg atgcctcatc tacaacgtca aggtaagttt aaacagttcg gtactaacta
     8161 accatacata tttaaatttt cagatccgtg gagtcaactt cccatccaac ggaccagtca
     8221 tgcaaaagaa gaccctcgga tgggaggcct ccaccgagac cctctaccca gccgacggag
     8281 gacttgaggg acgtgccgac atggccctca agctcgtcgg aggaggacac ctcatctgca
     8341 acctcaaggt aagtttaaac atgattttac taactaacta atctgattta aattttcaga
     8401 ccacctaccg ttccaagaag ccagccaaga acctcaagat gccaggagtc tactacgtcg
     8461 accgtcgtct tgagcgtatc aaggaggccg acaaggagac ctacgtcgag caacacgagg
     8521 tcgccgtcgc ccgttactgc gacctcccat ccaagctcgg acaccgtggt ggaggaggat
     8581 ctggaggagg aggatccatg gtttccgagt tgatcaagga gaatatgcac atgaaactct
     8641 acatggaagg aaccgtcaac aatcatcatt tcaaatgtac ttcagaagga gaaggaaaac
     8701 catacgaggg aactcagacc atgagaatca aggctgttga gggaggtaag tttaaaaaat
     8761 acgtgttcat aacttgatac gaatataaat tttcaggacc acttccattc gcgttcgaca
     8821 ttcttgccac ttcgttcatg tatggaagca agactttcat taatcacaca caagggatcc
     8881 cggatttctt caagcaatct ttcccagaag gattcacatg ggagagagtt accacttacg
     8941 aggatggagg agttcttacc gccacccaag acacatccct ccaaggtaag tttaaaagat
     9001 tatagcaatg ttcgtctaaa tataataaat tttcagatgg atgtctgatc tacaacgtca
     9061 agatcagagg agtcaatttc ccatcaaatg gaccagttat gcagaagaag actttgggct
     9121 gggaggcttc caccgagacc ctttacccag cagatggagg tctcgagggc cgcgctgata
     9181 tggctctcaa actcgtcgga ggaggacact tgatctgcaa tttaaaaacc acctaccggt
     9241 cgaaggtaag tttaaaatca atttttaata gcttatataa taatacaaat tttcagaagc
     9301 cagccaagaa tctcaaaatg ccaggagttt actatgttga ccgtcgtctt gagcgcatta
     9361 aagaggccga taaggaaacg tacgttgaac aacatgaagt tgccgttgct cgatattgtg
     9421 atctcccatc caagctcgga caccgtgccg cggccgctac ccgaagtatg tcaaaaagag
     9481 gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
     9541 ggcatatatg atgtcaatat ctccggttcg gtaagcacaa ccatgcagaa tgaagcccgt
     9601 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
     9661 tttattgaaa tgaacggctc ttttgccgac gagaacaggg gctggtgaaa tgcagtttaa
     9721 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
     9781 tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga
     9841 taaagtcccc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat
     9901 gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag
     9961 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
    10021 aggctccctt atacacagcc agtctgcagg tcgaatcgat gcgcgatgca agtcggcgat
    10081 ttctttgaag ttttgtcaaa agaagagaca aggacttgat agatacgctc atcagagtga
    10141 gattcattgc aaatagtgtg taaatcgtgg agagacgaga gaaaaggtgt gagaagctta
    10201 gaagataaag aataggaaga agaacatatc tcatttccta ttttctctaa tttttcgttc
    10261 atttttctag acattaccca tttgatggtt tttcaatatg gttatcaggg tactcatttt
    10321 ggtagttttc aaagcaattt ggagtttttt tttaaattgt caactacaaa aacgcagctt
    10381 aaaggtccaa atatcatagt ataaaaattc tttaaaaaat tccaaatttt atatgatttt
    10441 ttgaaaattg gaagaatgtt agtttttgcc atttttttgg aagtttttct ccggatttta
    10501 tggtacttat atttcaaata taaatttttg taggaattcg agtatgcgag agagctatag
    10561 tttactaaaa aaaagtggca aaaactcagt aattgccact tttttcaaaa atgttttgaa
    10621 aaattttact atgatatttg gccattttgg caccatatgt acgttacttt ttacgaaaca
    10681 ctctaatttt tgaaatttga gtttcatgtt taatcttttg tataaaattt ttatggtcct
    10741 tttgtgacag gataaaaaat tgcaaaaaaa tagaaaactt tgtttcgtga aaacacctag
    10801 tttaaaaatt gtgggccact tagcgattct agtcaaaaat cagacaaatg tcagctgccg
    10861 taactttttc cggatcaggg ttatcaaatt ttaattttaa ttgtaaatgt agaaaaatga
    10921 tcggcaacat tttgctaatt gaaaatattt tgaaaattgc tatagtacat attaatctag
    10981 tattaatata gtattaatgt tataatatca gttatgtgtt tgaataactc aagcgtaagt
    11041 ttccgtaaaa tatctttaaa ttttggtaat tttttacggt ttggtataaa tttttgcatt
    11101 ttttgatttt ctacactttt tttcgatttc gcattttccg aatttctctg aaataaatcg
    11161 aacaatcgga aaattttcga gggcaccctt agccatgcag ctcaggaatt tgactttatg
    11221 ttcccattca aattttttta aaattaaata aaatgctaat tgtaagataa attacaaaaa
    11281 ttattaaatc tcatcatcct tatgatgata gtgcagtgca tgaatatatt tttccaaata
    11341 ggatatgatt cgtcgagtag cttcacgtaa tccgttttct tcaatcacca tttcgaactg
    11401 caattttttt tttggatttt tgtaacaata ttgtcataaa ttgtgatttt gatattttgc
    11461 gtagagcatc agaacctttc tgctttgaat tttaatggga ttaaaaatta taaaaaactt
    11521 gcaccctaac acgtgacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
    11581 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
    11641 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
    11701 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
    11761 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
    11821 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
    11881 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
    11941 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
    12001 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    12061 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
    12121 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    12181 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    12241 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    12301 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
    12361 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    12421 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    12481 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    12541 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    12601 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    12661 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    12721 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    12781 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    12841 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    12901 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    12961 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    13021 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    13081 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    13141 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    13201 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    13261 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
    13321 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    13381 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    13441 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    13501 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    13561 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    13621 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    13681 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    13741 caacgcggcc tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc
    13801 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccag
    13861 cggataacaa tttcacaggg cc
//
