LOCUS       sequence-201392-       11244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of 
            antibody VH domains using SapI restriction enzyme. Abolished 
            Fc-mediated immune effector functions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Vazquez-Lombardi R, Nevoltris D, Rouet R, Christ D
  TITLE     Expression of IgG Monoclonals with Engineered Immune Effector 
            Functions.
  JOURNAL   Methods Mol Biol. 2018;1827:313-334. doi: 
            10.1007/978-1-4939-8648-4_16.
  PUBMED    30196504
REFERENCE   2  (bases 1 to 11244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2018"; volume: "1827:313-334. doi"; pages: " 
            10.1007/978-1-4939-8648-4_16"
COMMENT     Sequence Label: pRVL-3
FEATURES             Location/Qualifiers
     source          1..11244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     538..558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     584..608
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      629..638
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1728..1862
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1752..1771)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1806..1825
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2005..2027)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2587..4376
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(4678..6603)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7038..7056)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7124..7228
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7229..7297,7298..8089)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7447..7466)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8260..8848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8749..8768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9279..9424
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9467..10504
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(10502..10521)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10546..10594
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccgccgc caccatgggc tggagctgca tcatcctgtt
      661 cctggtggcc accgccaccg gcgtgcacag cgaagagcta cagagtgagc tcttcgccaa
      721 gaccaccgcc cccagcgtgt accccctggc ccccgtgtgc ggcggcacca ccggcagcag
      781 cgtgaccctg ggctgcctgg tgaagggcta cttccccgag cccgtgaccc tgacctggaa
      841 cagcggcagc ctgagcagcg gcgtgcacac cttccccgcc ctgctgcaga gcggcctgta
      901 caccctgagc agcagcgtga ccgtgaccag caacacctgg cccagccaga ccatcacctg
      961 caacgtggcc caccccgcca gcagcaccaa ggtggacaag aagatcgagc ctagggtgcc
     1021 catcacccag aacccctgcc cccctctgaa ggagtgccca ccctgcgccg cccccgacgc
     1081 agagggcgct cccagcgtgt tcatcttccc ccccaagatc aaggacgtgc tgatgatcag
     1141 cctgagcccc atggtgacct gcgtggtggt ggacgtgagc gaggacgacc ccgacgtgca
     1201 gatcagctgg ttcgtgaaca acgtggaggt gcacaccgcc cagacccaga cccacaggga
     1261 ggactacaac agcaccctga gggtggtgag cgccctgccc atccagcacc aggactggat
     1321 gagcggcaag gccttcgcat gcgctgtgaa caacagggcc ctgccaagcc ctatcgagaa
     1381 gaccatcagc aagcccaggg gccccgtgag ggccccccag gtgtacgtgc tgcctccccc
     1441 agccgaggag atgaccaaga aggagttcag cctgacctgc atgatcaccg gcttcctgcc
     1501 cgccgagatc gccgtggact ggaccagcaa cggcaggacc gagcagaact acaagaacac
     1561 cgccaccgtg ctggacagcg acggcagcta cttcatgtac agcaagctga gggtgcagaa
     1621 aagcacctgg gagaggggca gcctgttcgc ctgcagcgtg gtgcacgagg gcctgcacaa
     1681 ccacctgacc accaagacca tcagcaggag cctgggcaag tgataaggat ccagacatga
     1741 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     1801 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     1861 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     1921 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatccg gctgcctcgc
     1981 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     2041 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     2101 cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat cgatccccgc cccggacgaa
     2161 ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt aatcccttca
     2221 gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg gggggaccaa
     2281 acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc acatttgcca
     2341 acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca acacaacatt
     2401 gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac atgtacctcc
     2461 caggggccca ggaagactac gggaggctac accaacgtca atcagagggg cctgtgtagc
     2521 taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc ccccttgtta
     2581 accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag actaacccta
     2641 attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg ttagtaaaag
     2701 ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta tttacatggg
     2761 gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa
     2821 ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct tcgtttagct
     2881 aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt
     2941 ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc attgtgctat
     3001 gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag gaatgaaaca
     3061 ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa agactggatg
     3121 tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa tatgatactg
     3181 gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt gttacactct
     3241 atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact ggttgtctct
     3301 aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca aagacaagtg
     3361 gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac acgccgccct
     3421 gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc ccgctaacca
     3481 ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat acctgcataa
     3541 gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca aattacacac
     3601 acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag gtcgctgaga
     3661 gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg gatagcatat
     3721 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     3781 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     3841 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     3901 gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag ggtagtatat
     3961 gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata gcatatgcta
     4021 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gcataggcta
     4081 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     4141 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     4201 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta
     4261 tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt gctatccttt
     4321 gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt ttcctgctgg
     4381 ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca tgtctgattg
     4441 ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc gcggagctga
     4501 gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa aatggtgaca
     4561 agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga tttattcttt
     4621 agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca agttacatca
     4681 ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat ctccgtcatc
     4741 accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat ctactccatc
     4801 gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg tcataacaag
     4861 gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa agaccatgaa
     4921 ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag gggccattcc
     4981 aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg cagttcctcg
     5041 ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg cgacccaagt
     5101 tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta aaccttctgc
     5161 aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa accaccctcc
     5221 ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc cttctcctgg
     5281 gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac catctgggcc
     5341 accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa atggccttct
     5401 acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac tgggactcct
     5461 gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac ttccccccct
     5521 ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc ggcctccact
     5581 acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc ctgctcctgc
     5641 ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc
     5701 tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc
     5761 tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc
     5821 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     5881 tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgcccctc ctgcccctcc
     5941 tcctgctcct gcccctcctg ctcctgcccc tcctgcccct cctgcccctc ctgctcctgc
     6001 ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     6061 tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc
     6121 tcctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc
     6181 ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc
     6241 tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc
     6301 tcctgctcct gcccctcccg ctcctgctcc tgctcctgtt ccaccgtggg tccctttgca
     6361 gccaatgcaa cttggacgtt tttggggtct ccggacacca tctctatgtc ttggccctga
     6421 tcctgagccg cccggggctc ctggtcttcc gcctcctcgt cctcgtcctc ttccccgtcc
     6481 tcgtccatgg ttatcacccc ctcttctttg aggtccactg ccgccggagc cttctggtcc
     6541 agatgtgtct cccttctctc ctaggccatt tccaggtcct gtacctggcc cctcgtcaga
     6601 catgattcac actaaaagag atcaatagac atctttatta gacgacgctc agtgaataca
     6661 gggagtgcag actcctgccc cctccaacag cccccccacc ctcatcccct tcatggtcgc
     6721 tgtcagacag atccaggtct gaaaattccc catcctccga accatcctcg tcctcatcac
     6781 caattactcg cagcccggaa aactcccgct gaacatcctc aagatttgcg tcctgagcct
     6841 caagccaggc ctcaaattcc tcgtccccct ttttgctgga cggtagggat ggggattctc
     6901 gggacccctc ctcttcctct tcaaggtcac cagacagaga tgctactggg gcaacggaag
     6961 aaaagctggg tgcggcctgt gaggatcagc ttatcgatga taagctgtca aacatgagaa
     7021 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     7081 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     7141 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7201 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7261 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7321 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7381 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7441 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     7501 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7561 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7621 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7681 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7741 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     7801 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7861 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7921 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7981 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8041 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8101 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8161 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8221 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8281 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8341 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8401 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8461 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8521 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8581 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8641 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8701 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8761 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8821 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8881 ggccttttgc tggccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat
     8941 ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc
     9001 catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccccgagatg
     9061 cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt
     9121 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg
     9181 ttagcgaggt gccgccctgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt
     9241 ccccggaaga aatatatttg catgtcttta gttctatgat gacacaaacc ccgcccagcg
     9301 tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca
     9361 cttcgcatat taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc
     9421 ttaacagcgt caacagcgtg ccgcagatcc cggggggcaa tgagatatga aaaagcctga
     9481 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     9541 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     9601 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     9661 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     9721 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     9781 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     9841 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     9901 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     9961 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
    10021 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
    10081 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
    10141 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
    10201 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
    10261 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
    10321 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
    10381 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
    10441 cgccgatagt ggaaaccgac gccccagcac tcgtccggat cgggagatgg gggaggctaa
    10501 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
    10561 agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
    10621 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
    10681 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
    10741 gcggcaggcc ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga
    10801 atggtttatg gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact
    10861 ggactgagca gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc
    10921 gcgacacgaa caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc
    10981 gcggatttct ggcgtgccaa gctagtcgac caattctcat gtttgacagc ttatcatcgc
    11041 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
    11101 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
    11161 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
    11221 gctcatgtcc aatatgaccg ccat
//
