LOCUS       sequence-201402-        6557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains YY1 binding sites for in vitro ligation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Weintraub AS, Li CH, Zamudio AV, Sigova AA, Hannett NM, Day DS, 
            Abraham BJ, Cohen MA, Nabet B, Buckley DL, Guo YE, Hnisz D, Jaenisch
            R, Bradner JE, Gray NS, Young RA
  TITLE     YY1 Is a Structural Regulator of Enhancer-Promoter Loops.
  JOURNAL   Cell. 2017 Dec 14;171(7):1573-1588.e28. doi: 
            10.1016/j.cell.2017.11.008. Epub 2017 Dec 7.
  PUBMED    29224777
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.11.008"; journalName: "Cell"; date: 
            "2017-12-14- 14"; volume: "171"; issue: "7"; pages: "1573-1588.e28"
COMMENT     Sequence Label: pAW49.pUC19.YY1
FEATURES             Location/Qualifiers
     source          1..6557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    120..141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(156..161,162..179,180..186)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    194..210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     199..221
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     218..234
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     218..234
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4175..4192)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4175..4191)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4184..4206)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4400..4419)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4519..4541
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4579..4597)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4665..4769
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4770..4838,4839..5630)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4988..5007)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5801..6389
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6290..6309
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(6543..6557,1..3)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
       61 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      121 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      181 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
      241 tacgccaagc ttgcatgcct gcaggggatc cgtaagctag gctagaggca ggcggatttc
      301 cgagttcgag gccagcctgg tctacagagt gagttccagg acagccagga ctacacaacg
      361 taagggaaac cctggctcag aaaaacaaaa actgaaaact aaccaacaga gtagacagag
      421 caacaacaac aacgaaccgg ttcgctcccc ggccatcttg gcggctggtg tgtcgacaga
      481 ggcagcaaaa gccagaaacg aacgcatcat caagtgccgg tcgtgcagct tcctcggcaa
      541 cggcggcaga aaattctgcc agggcggcaa aaacgtccga gacgaatgcc aggtcatctg
      601 aaacagcagc ggaacggagc gcctctgcag cggcagacgc aaaaacagcg gcggcgggga
      661 gtgcgtcaac ggcatccacg aaggcgacag aggctgcggg aagtgcggta tcagcatcgc
      721 agagcaaaag tgcggcagaa gcggcggcaa tacgtgcaaa aaattcggca aaacgagctc
      781 cagctaatac tttcacacga agagtatgtg accttgattg ttatcatctt gagctgaggg
      841 ttgattgctt gttaatttaa cttgagaata cttagcattt ttctcttttt taaaaaaata
      901 aataacacag tgagaacctt tcagtgtgtc actgagctgt gtgtgtctgt tgggagagct
      961 tgaggctatg agagctgtgt gtgaacaggg ctgtgcacag gtggtctcag tcatttattt
     1021 tctttagttc actgtgtaac atccgggctc tactctctca ctatgaagca cataccgaga
     1081 gtctgttcaa ggacacagtg aaggcctgag ccttgtcctg tccccacttt gacaattaat
     1141 tgctggcctt tggaaagtgg aaacagctgt gatgtcaaca aagagttcgg actacaggaa
     1201 gataatttag agctcaatga tgtggtcatc actatctgtg cctgtgatga gatacggaaa
     1261 tatcgacatg atagtcggag aaatatgttg gagtggtgcc tgccagtgat tgatgccaaa
     1321 aataagagtg gcagcctgga gttcagcatt cctgggcagc ctaatgactt cttccctgtt
     1381 caagtttctt tcatctccaa acagaattac tgcaacacac aggttaccaa agtgacccag
     1441 gtagataaac cgtcccgtca ggttttccac ggagaccact ttcttagtga caaatacgaa
     1501 atcctgtaat accaagaagg gaaagctgaa aaggaaggat ttcagattaa taaagaagaa
     1561 gccaaaagtg gctgaagagt ttttccaaat ttacaagcat tggaggtccc cccaaacccc
     1621 ttttctgata cagtacacgt tctctgtgcg caaggaccct caactcacac ccagtgttgc
     1681 aatgtcacag agacattttt gacaaatgac actgacataa agggaaaggc tgctgatttc
     1741 tttggccagc aggaagccag ctctccaggg aatggatgcc ccagtaagga gctcttctgc
     1801 cttcacctaa gtctcccttt attttaagcc ctaaatatag gtaggattat gagcccccaa
     1861 gggctaaaag aactcatctg gggaccttta aagtgctgtg tgcacaagat aggtcatgtc
     1921 acatttcctt ccacttgatt gtagcttcta atgatgcagt cgtgaagctg ccctgggcgc
     1981 tccctgtagc cctgctactg tgattttctg cttctccttc tttccagggt cttttacagg
     2041 tcaggggtct tatgtgattt cctgtttttc aggagcgcat ccacagctga gataagaacc
     2101 tgttggctta gatttttcaa aaccgatttt tcataagggt tcttacatgc tttgtttgtt
     2161 tgtttgtttt ttgagccagg gtttctctgt gtagtcctgg ctgtcctgga actcactttg
     2221 tagaccaggc tggtcttgaa ctcagaaatt tgcctgcctc tgcctcccaa gtaccggctg
     2281 ttcttacatg ctttatcctc ccttctcacc cactaacagc aaagaaggac attaggatag
     2341 gggggaagtt tgtgtagaaa caatgtgttg gagcaaatct aatttgtgtt gtcaggatag
     2401 cagcagttta gttcacaaaa atcagcaaag tcgggtcaat taagaagaag ctgggaggct
     2461 cagctgtttg ctgtttggag gcagttcctt ggggtgattt cagtctctgt caggatacca
     2521 gcagtccagt tcaatagtgt caggattgca aacatgaatc aggaggggtg gcatgacaca
     2581 gcagaaacag tcaggtctcc aatgattcaa caagagtcag caggagggac cagggccagc
     2641 agagatgcca gaacttctct gtcctgcctc tctcaacaaa gtgaaagcct aaagaagaca
     2701 caagaccaat gaagcattgt aaagctggct atgcaagccc acactgtccc ttgagtccta
     2761 ttatactccc tacaaacatc gtgtgtcctc ccacgggtct agcctcgtgt tgcctcagca
     2821 aaattccgag tctgtatcag ctgatgtata aactcccact tcactctgcc agtcagccca
     2881 agtctgcaga agcagcaaga agccgccaga acatcaccag aagttttttg gtgcatttat
     2941 ctctaatcac caataaacat aagtgttaca ttattatttt tttaatccta ttttaaagac
     3001 atgcgtttta aatgctttaa cctctctttt aacccaacac ccagagttag tacaaaagaa
     3061 tgagaggagg gggaagtgga cctgtttaga aaggttcttt gaggaactcc tgtcagtgtt
     3121 ggctggaaac agtcagttca gttcacaggg taagagcagc tgcttcactc aaggttcacg
     3181 gatacatcag cagtccagtg tggtagagtc aaacgggaat cggctggggt ggccctacct
     3241 ggtgagactg ccaggcttca gctttgggcc tagttagcag gaggacctgg aggaatgtca
     3301 ggagaagttt tcagagcatc atacagctag ctgtaccagc aagccaagct cagcccaagt
     3361 cactgtccgt tgagtcctag ttatgttctc caatcatcat gtgtcctgtg tcttgcctca
     3421 gcacttgcac ctgtctcagc tggcatcact ctgccaacca ccctgagtca gctagagcaa
     3481 caagaaacta acacaccacc caaaattttt tggtgtcttg ctctctatag aggtcctaac
     3541 acatgcagct caactacgca gtataagaga gatacatgtc tgttgttaga gaaaaatcct
     3601 tcaagagacc aagcggcaga ggctcctgtc aggggcggca ggtccggacg gccagacacc
     3661 tctctgggca gcagcccgag tccctgcaga gtcagagcgg ccgcacccca cctgcggcct
     3721 catgctgttg gcagcagcag tctgcacgaa tgcgagaaag gctattgttt ctcgacagtt
     3781 cgtggagatg acccgaactc ggattgaagg cttattagca gctttcccaa aactcatgaa
     3841 cactggaaaa cagcatactt ttgttgaaac agagagtgta agatatgtgt accaacctag
     3901 aaactgtact attgactagt ctcttctctc ttaaaatggc tgcctgtctg ccatggctca
     3961 ggagctgagg cagtaggctc acatatttcg aggctagctt ggatatctta gtttcctttc
     4021 tgtgcactgt ggtaaaaata ctaaaagcaa actgaggaat agaattacaa tcgacctcag
     4081 acaagcagca gtcaggacaa gaactcaagg ctcaagacga ggatcccccg ggtaccgagc
     4141 tcgaattcac tggccgggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg
     4201 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     4261 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     4321 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     4381 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     4441 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     4501 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     4561 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     4621 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4681 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4741 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4801 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4861 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4921 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4981 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5041 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5101 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5161 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5221 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5281 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5341 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5401 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5461 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5521 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5581 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5641 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5701 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5761 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5821 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5881 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5941 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6001 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6061 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6121 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6181 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6241 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6301 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6361 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6421 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6481 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6541 gcagcgagtc agtgagc
//
