LOCUS       sequence-201404-        6530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  No YY1 binding motifs as control for in vitro ligation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Weintraub AS, Li CH, Zamudio AV, Sigova AA, Hannett NM, Day DS, 
            Abraham BJ, Cohen MA, Nabet B, Buckley DL, Guo YE, Hnisz D, Jaenisch
            R, Bradner JE, Gray NS, Young RA
  TITLE     YY1 Is a Structural Regulator of Enhancer-Promoter Loops.
  JOURNAL   Cell. 2017 Dec 14;171(7):1573-1588.e28. doi: 
            10.1016/j.cell.2017.11.008. Epub 2017 Dec 7.
  PUBMED    29224777
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.11.008"; journalName: "Cell"; date: 
            "2017-12-14- 14"; volume: "171"; issue: "7"; pages: "1573-1588.e28"
COMMENT     Sequence Label: pAW79.pUC19.noYY1
FEATURES             Location/Qualifiers
     source          1..6530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(141..146,147..164,165..171)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    179..195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     184..206
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     203..219
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     203..219
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4133..4150)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4133..4149)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4142..4164)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4358..4377)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4477..4499
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4537..4555)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4623..4727
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4728..4796,4797..5588)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4946..4965)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5759..6347
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6248..6267
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6501..6518
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
       61 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      121 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      181 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca
      241 tgcctgcagg ggatccgtaa gctaggctag aggcaggcgg atttccgagt tcgaggccag
      301 cctggtctac agagtgagtt ccaggacagc caggactaca caacgtaagg gaaaccctgg
      361 ctcagaaaaa caaaaactga aaactaacca acagagtaga cagagcaaca acaacaacga
      421 accggtaggc agtggctagc tcacgtccct ggatcggtcg acagaggcag caaaagccag
      481 aaacgaacgc atcatcaagt gccggtcgtg cagcttcctc ggcaacggcg gcagaaaatt
      541 ctgccagggc ggcaaaaacg tccgagacga atgccaggtc atctgaaaca gcagcggaac
      601 ggagcgcctc tgcagcggca gacgcaaaaa cagcggcggc ggggagtgcg tcaacggcat
      661 ccacgaaggc gacagaggct gcgggaagtg cggtatcagc atcgcaagca aaagtgcggc
      721 agaagcggcg gcaatacgtg caaaaaattc gggaaaacga gctccagcta atactttcac
      781 acgaagagta tgtgaccttg attgttatca tcttgagctg agggttgatt gcttgttaat
      841 ttaacttgag aatacttagc atttttctct tttttaaaaa aataaataac acagtgagaa
      901 cctttcagtg tgtcactgag ctgtgtgtgt ctgttgggag agcttgaggc tatgagagct
      961 gtgtgtgaac agggctgtgc acaggtggtc tcagtcattt attttcttta gttcactgtg
     1021 taacatccgg gctctactct ctcactatga agcacatacc gagagtctgt tcaaggacac
     1081 agtgaaggcc tgagccttgt cctgtcccca ctttgacaat taattgctgg cctttggaaa
     1141 gtggaaacag ctgtgatgtc aacaaagagt tcggactaca ggaagataat ttagagctca
     1201 atgatgtggt catcactatc tgtgcctgtg atgagatacg gaatatcgac atgatagtcg
     1261 gagaaatatg ttggagtggt gcctgccagt gattgatgcc aaaaataaga gtggcagcct
     1321 ggagttcagc attcctgggc agcctaatga cttcttccct gttcaagttt ctttcatctc
     1381 caaacagaat tactgcaaca cacaggttac caaagtgacc caggtagata aaccgtcccg
     1441 tcaggttttc cacggagacc actttcttag tgacaaatac gaaatcctgt aataccaaga
     1501 agggaaagct gaaaaggaag gatttcagat taataaagaa gaagccaaaa gtggctgaag
     1561 agtttttcca aatttacaag cattggaggt ccccccaaac cccttttctg atacagtaca
     1621 cgttctctgt gcgcaaggac cctcaactca cacccagtgt tgcaatgtca cagagacatt
     1681 tttgacaaat gacactgaca taaagggaaa ggctgctgat ttctttggcc agcaggaagc
     1741 cagctctcca gggaatgaat gccccagtaa gtagctcttc tgccttcacc taagtctccc
     1801 tttattttaa gccctaaata taggtaggat tatgagcccc caagggctaa aagaactcat
     1861 ctggggacct ttaaagtgct gtgtgcacaa gataggtcat gtcacatttc cttccacttg
     1921 attgtagctt ctaatgatgc agtcgtgaag ctgccctggg cgctccctgt agccctgcta
     1981 ctgtgatttt ctgcttctcc ttctttccag ggtcttttac aggtcagggg tcttatgtga
     2041 tttcctgttt ttcaggagcg catccacagc tgagataaga acctgttggc ttagattttt
     2101 caaaaccgat ttttcataag ggttcttaca tgctttgttt gtttgtttgt tttttgagcc
     2161 agggtttctc tgtgtagtcc tggctgtcct ggaactcact ttgtagacca ggctggtctt
     2221 gaactcagaa atttgcctgc ctctgcctcc caagtgccgg ctgttcttac atgctttatc
     2281 ctcccttctc acccactaac agcaagagaa aggacattag gatagggggg aagtttgtgt
     2341 agaaacaatg tgttggagca aatctaattt gtgttgtcag gatagcagca gtttagttca
     2401 caaaaatcag caaagtcggg tcaattaaga agaagctggg aggctcagct gtttgctgtt
     2461 tagaagcagt tccttggggt gatttcagtc tctgtcagga taccagcagt ccagttcaat
     2521 agtgtcagga ttgcaaacat gaatcaggag gggtggcatg acacagcaga aacagtcagg
     2581 tctccaatga ttcaacaaga gtcagcagga gggaccaggg ccagcagaga tgccagaact
     2641 tctctgtcct gcctctctca acaaagtgaa agcctaaaga agacacaaga ccaatgaagc
     2701 attgtaaagc tggctatgca agcccacact gtcccttgag tcctattata ctccctacaa
     2761 acatcgtgtg tcctcccacg ggtctagcct ctgttgcctc agcaaaattc cgagtctgta
     2821 tcagctgatg tataaactcc cacttcactc tgccagtcag cccaagtctg cagaagcagc
     2881 aagaagccgc cagaacatca ccagaagttt tttggtgcat ttatctctaa tcaccaataa
     2941 acataagtgt tacattatta tttttttaat cctattttaa agacatgcgt tttaaatgct
     3001 ttaacctctc ttttaaccca acacccagag ttagtacaaa agaatgagag gagggggaag
     3061 tggacctgtt tagaaaggtt ctttgaggaa ctcctgtcag tgttggctgg aaacagtcag
     3121 ttcagttcac agggtaagag cagctgcttc actcaaggtt cacggataca tcagcagtcc
     3181 agtgtgctag agccaaacgg gaatcggctg gggtggccct acctggtgag actgccaggc
     3241 ttcagcttgg ggccctagtt agcaggagga cctggaggaa tgtcagcaga agttttcaga
     3301 gcatcataca gctagctgta ccagcaagcc aagctcagcc aagtcactgt ccgttgagtc
     3361 ctagttatgt tctccaatca tcatgtgtcc tgtgtcttgc ctcagcactt gcacctgtct
     3421 cagctggcat cactctgcca accaccctga gtctgctaaa gcaacaagaa actaacacac
     3481 cacccaaaat tttttggtgt cttgctctct atagagtcct aacacatgca gctcaactac
     3541 gcagtataag agagatacat gtctgttgtt agagaaaaat ccttcaagag gccaagcggc
     3601 agaggctcct gtcaggggcg gcaggtccgg agcggccaga cacctctctg ggcagcagcc
     3661 cgagtccctg cagagtcaga gcggccgcac cccacctgcg gcctcatgct gttggcagca
     3721 gcagtctgca cgaatgcgag aaaggctatt gtttctcgac agttcgtgga gatgacccga
     3781 actcggattg aaggcttatt agcagctttc ccaaaactca tgaacactgg aaaacagcat
     3841 acttttgttg aaacagagag tgtaagatat gtgtaccaac ctagaaactg tactattgac
     3901 tagtcccgcc tccccaggcc tacgcgctga gctccacgtg ctcaggagct gaggcagtag
     3961 gctcacatat ttcgaggcta gcttggatat cttagtttcc tttctgtgca ctgtggtaaa
     4021 aatactaaaa gcaaactgag gaatagaatt acaatcgacc tcagacaagc agcagtcagg
     4081 acaagaactc aaggctcaag acgaggatcc cccgggtacc gagctcgaat tcactggccg
     4141 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     4201 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     4261 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     4321 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     4381 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     4441 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     4501 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     4561 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4621 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4681 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4741 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4801 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4861 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4921 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4981 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5041 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5101 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5161 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5221 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5281 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5341 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5401 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5461 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5521 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5581 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5641 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5701 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5761 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5821 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5881 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5941 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6001 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6061 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6121 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6181 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6241 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6301 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6361 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6421 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6481 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
//
