LOCUS       sequence-201431-        9085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for catalytically inactive Cpf1 from 
            Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Tak YE, Kleinstiver BP, Nunez JK, Hsu JY, Horng JE, Gong J, Weissman
            JS, Joung JK
  TITLE     Inducible and multiplex gene regulation using CRISPR-Cpf1-based 
            transcription factors.
  JOURNAL   Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483.
  PUBMED    29083402
REFERENCE   2  (bases 1 to 9085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483."
COMMENT     Sequence Label: JG674: CAG-human
            dLbCpf1(D832A)-NLS-3xHA-3xFLA...
FEATURES             Location/Qualifiers
     source          1..9085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1777..5460
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             5461..5508
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5515..5595
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5602..5667
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5695..6021
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLEG"
     primer_bind     complement(6107..6126)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6172..6227
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6226..6245)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6588..6604)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6588..6604)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6601..6623)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6612..6628
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6636..6642,6643..6660,6661..6666))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6681..6702
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6761..6956
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6807..6942
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6962..7096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6999..7018)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7053..7072
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7164..7181)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7335..7923)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7415..7434)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8094..8885,8886..8954))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8717..8736
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8955..9059)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cggggggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tctgcagtcg
     1741 acggtaccgc gggcccggga tccaccggtc gccaccatga gcaagctgga gaagtttaca
     1801 aactgctact ccctgtctaa gaccctgagg ttcaaggcca tccctgtggg caagacccag
     1861 gagaacatcg acaataagcg gctgctggtg gaggacgaga agagagccga ggattataag
     1921 ggcgtgaaga agctgctgga tcgctactat ctgtctttta tcaacgacgt gctgcacagc
     1981 atcaagctga agaatctgaa caattacatc agcctgttcc ggaagaaaac cagaaccgag
     2041 aaggagaata aggagctgga gaacctggag atcaatctgc ggaaggagat cgccaaggcc
     2101 ttcaagggca acgagggcta caagtccctg tttaagaagg atatcatcga gacaatcctg
     2161 ccagagttcc tggacgataa ggacgagatc gccctggtga acagcttcaa tggctttacc
     2221 acagccttca ccggcttctt tgataacaga gagaatatgt tttccgagga ggccaagagc
     2281 acatccatcg ccttcaggtg tatcaacgag aatctgaccc gctacatctc taatatggac
     2341 atcttcgaga aggtggacgc catctttgat aagcacgagg tgcaggagat caaggagaag
     2401 atcctgaaca gcgactatga tgtggaggat ttctttgagg gcgagttctt taactttgtg
     2461 ctgacacagg agggcatcga cgtgtataac gccatcatcg gcggcttcgt gaccgagagc
     2521 ggcgagaaga tcaagggcct gaacgagtac atcaacctgt ataatcagaa aaccaagcag
     2581 aagctgccta agtttaagcc actgtataag caggtgctga gcgatcggga gtctctgagc
     2641 ttctacggcg agggctatac atccgatgag gaggtgctgg aggtgtttag aaacaccctg
     2701 aacaagaaca gcgagatctt cagctccatc aagaagctgg agaagctgtt caagaatttt
     2761 gacgagtact ctagcgccgg catctttgtg aagaacggcc ccgccatcag cacaatctcc
     2821 aaggatatct tcggcgagtg gaacgtgatc cgggacaagt ggaatgccga gtatgacgat
     2881 atccacctga agaagaaggc cgtggtgacc gagaagtacg aggacgatcg gagaaagtcc
     2941 ttcaagaaga tcggctcctt ttctctggag cagctgcagg agtacgccga cgccgatctg
     3001 tctgtggtgg agaagctgaa ggagatcatc atccagaagg tggatgagat ctacaaggtg
     3061 tatggctcct ctgagaagct gttcgacgcc gattttgtgc tggagaagag cctgaagaag
     3121 aacgacgccg tggtggccat catgaaggac ctgctggatt ctgtgaagag cttcgagaat
     3181 tacatcaagg ccttctttgg cgagggcaag gagacaaaca gggacgagtc cttctatggc
     3241 gattttgtgc tggcctacga catcctgctg aaggtggacc acatctacga tgccatccgc
     3301 aattatgtga cccagaagcc ctactctaag gataagttca agctgtattt tcagaaccct
     3361 cagttcatgg gcggctggga caaggataag gagacagact atcgggccac catcctgaga
     3421 tacggctcca agtactatct ggccatcatg gataagaagt acgccaagtg cctgcagaag
     3481 atcgacaagg acgatgtgaa cggcaattac gagaagatca actataagct gctgcccggc
     3541 cctaataaga tgctgccaaa ggtgttcttt tctaagaagt ggatggccta ctataacccc
     3601 agcgaggaca tccagaagat ctacaagaat ggcacattca agaagggcga tatgtttaac
     3661 ctgaatgact gtcacaagct gatcgacttc tttaaggata gcatctcccg gtatccaaag
     3721 tggtccaatg cctacgattt caacttttct gagacagaga agtataagga catcgccggc
     3781 ttttacagag aggtggagga gcagggctat aaggtgagct tcgagtctgc cagcaagaag
     3841 gaggtggata agctggtgga ggagggcaag ctgtatatgt tccagatcta taacaaggac
     3901 ttttccgata agtctcacgg cacacccaat ctgcacacca tgtacttcaa gctgctgttt
     3961 gacgagaaca atcacggaca gatcaggctg agcggaggag cagagctgtt catgaggcgc
     4021 gcctccctga agaaggagga gctggtggtg cacccagcca actcccctat cgccaacaag
     4081 aatccagata atcccaagaa aaccacaacc ctgtcctacg acgtgtataa ggataagagg
     4141 ttttctgagg accagtacga gctgcacatc ccaatcgcca tcaataagtg ccccaagaac
     4201 atcttcaaga tcaatacaga ggtgcgcgtg ctgctgaagc acgacgataa cccctatgtg
     4261 atcggcatcg ccaggggcga gcgcaatctg ctgtatatcg tggtggtgga cggcaagggc
     4321 aacatcgtgg agcagtattc cctgaacgag atcatcaaca acttcaacgg catcaggatc
     4381 aagacagatt accactctct gctggacaag aaggagaagg agaggttcga ggcccgccag
     4441 aactggacct ccatcgagaa tatcaaggag ctgaaggccg gctatatctc tcaggtggtg
     4501 cacaagatct gcgagctggt ggagaagtac gatgccgtga tcgccctgga ggacctgaac
     4561 tctggcttta agaatagccg cgtgaaggtg gagaagcagg tgtatcagaa gttcgagaag
     4621 atgctgatcg ataagctgaa ctacatggtg gacaagaagt ctaatccttg tgcaacaggc
     4681 ggcgccctga agggctatca gatcaccaat aagttcgaga gctttaagtc catgtctacc
     4741 cagaacggct tcatctttta catccctgcc tggctgacat ccaagatcga tccatctacc
     4801 ggctttgtga acctgctgaa aaccaagtat accagcatcg ccgattccaa gaagttcatc
     4861 agctcctttg acaggatcat gtacgtgccc gaggaggatc tgttcgagtt tgccctggac
     4921 tataagaact tctctcgcac agacgccgat tacatcaaga agtggaagct gtactcctac
     4981 ggcaaccgga tcagaatctt ccggaatcct aagaagaaca acgtgttcga ctgggaggag
     5041 gtgtgcctga ccagcgccta taaggagctg ttcaacaagt acggcatcaa ttatcagcag
     5101 ggcgatatca gagccctgct gtgcgagcag tccgacaagg ccttctactc tagctttatg
     5161 gccctgatga gcctgatgct gcagatgcgg aacagcatca caggccgcac cgacgtggat
     5221 tttctgatca gccctgtgaa gaactccgac ggcatcttct acgatagccg gaactatgag
     5281 gcccaggaga atgccatcct gccaaagaac gccgacgcca atggcgccta taacatcgcc
     5341 agaaaggtgc tgtgggccat cggccagttc aagaaggccg aggacgagaa gctggataag
     5401 gtgaagatcg ccatctctaa caaggagtgg ctggagtacg cccagaccag cgtgaagcac
     5461 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaaggg atcctaccca
     5521 tacgatgttc cagattacgc ttatccctac gacgtgcctg attatgcata cccatatgat
     5581 gtccccgact atgcctcgag cgactacaaa gaccatgacg gtgattataa agatcatgac
     5641 atcgattaca aggatgacga tgacaaggct gcaggaggcg gtggaagcgg gaggggagtg
     5701 caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc
     5761 gtggtgcact acaccgggat gcttgaagat ggaaagaaat ttgattcctc ccgggacaga
     5821 aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg
     5881 gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat
     5941 ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt cgatgtggag
     6001 cttctaaaac tggaaggata agcggccgca ctcctcaggt gcaggctgcc tatcagaagg
     6061 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
     6121 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     6181 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     6241 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     6301 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     6361 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     6421 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     6481 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     6541 tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt
     6601 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6661 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6721 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa
     6781 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6841 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6901 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6961 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7021 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7081 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7141 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7201 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7261 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7321 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7381 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7441 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7501 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7561 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7621 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7681 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7741 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7801 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7861 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7921 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7981 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8041 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8101 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8161 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8221 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8281 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8341 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8401 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8461 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8521 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8581 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8641 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8701 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8761 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8821 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8881 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8941 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9001 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9061 acatttcccc gaaaagtgcc acctg
//
