LOCUS       sequence-201436-       10093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for catalytically inactive Cpf1 from 
            Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10093)
  AUTHORS   Tak YE, Kleinstiver BP, Nunez JK, Hsu JY, Horng JE, Gong J, Weissman
            JS, Joung JK
  TITLE     Inducible and multiplex gene regulation using CRISPR-Cpf1-based 
            transcription factors.
  JOURNAL   Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483.
  PUBMED    29083402
REFERENCE   2  (bases 1 to 10093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483."
COMMENT     Sequence Label: YET1000: CAG-human
            dLbCpf1(D832A)-NLS-3xHA-3xF...
FEATURES             Location/Qualifiers
     source          1..10093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1777..5460
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             5461..5508
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5515..5595
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5602..5667
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5698..6018
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             6028..6348
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             6373..6693
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             6703..7029
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLEG"
     primer_bind     complement(7115..7134)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7180..7235
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7234..7253)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7596..7612)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7596..7612)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7609..7631)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7620..7636
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7644..7650,7651..7668,7669..7674))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7689..7710
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7769..7964
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7815..7950
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7970..8104
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8007..8026)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8061..8080
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8172..8189)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8343..8931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8423..8442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9102..9893,9894..9962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9725..9744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9963..10067)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tctgcagtcg
     1741 acggtaccgc gggcccggga tccaccggtc gccaccatga gcaagctgga gaagtttaca
     1801 aactgctact ccctgtctaa gaccctgagg ttcaaggcca tccctgtggg caagacccag
     1861 gagaacatcg acaataagcg gctgctggtg gaggacgaga agagagccga ggattataag
     1921 ggcgtgaaga agctgctgga tcgctactat ctgtctttta tcaacgacgt gctgcacagc
     1981 atcaagctga agaatctgaa caattacatc agcctgttcc ggaagaaaac cagaaccgag
     2041 aaggagaata aggagctgga gaacctggag atcaatctgc ggaaggagat cgccaaggcc
     2101 ttcaagggca acgagggcta caagtccctg tttaagaagg atatcatcga gacaatcctg
     2161 ccagagttcc tggacgataa ggacgagatc gccctggtga acagcttcaa tggctttacc
     2221 acagccttca ccggcttctt tgataacaga gagaatatgt tttccgagga ggccaagagc
     2281 acatccatcg ccttcaggtg tatcaacgag aatctgaccc gctacatctc taatatggac
     2341 atcttcgaga aggtggacgc catctttgat aagcacgagg tgcaggagat caaggagaag
     2401 atcctgaaca gcgactatga tgtggaggat ttctttgagg gcgagttctt taactttgtg
     2461 ctgacacagg agggcatcga cgtgtataac gccatcatcg gcggcttcgt gaccgagagc
     2521 ggcgagaaga tcaagggcct gaacgagtac atcaacctgt ataatcagaa aaccaagcag
     2581 aagctgccta agtttaagcc actgtataag caggtgctga gcgatcggga gtctctgagc
     2641 ttctacggcg agggctatac atccgatgag gaggtgctgg aggtgtttag aaacaccctg
     2701 aacaagaaca gcgagatctt cagctccatc aagaagctgg agaagctgtt caagaatttt
     2761 gacgagtact ctagcgccgg catctttgtg aagaacggcc ccgccatcag cacaatctcc
     2821 aaggatatct tcggcgagtg gaacgtgatc cgggacaagt ggaatgccga gtatgacgat
     2881 atccacctga agaagaaggc cgtggtgacc gagaagtacg aggacgatcg gagaaagtcc
     2941 ttcaagaaga tcggctcctt ttctctggag cagctgcagg agtacgccga cgccgatctg
     3001 tctgtggtgg agaagctgaa ggagatcatc atccagaagg tggatgagat ctacaaggtg
     3061 tatggctcct ctgagaagct gttcgacgcc gattttgtgc tggagaagag cctgaagaag
     3121 aacgacgccg tggtggccat catgaaggac ctgctggatt ctgtgaagag cttcgagaat
     3181 tacatcaagg ccttctttgg cgagggcaag gagacaaaca gggacgagtc cttctatggc
     3241 gattttgtgc tggcctacga catcctgctg aaggtggacc acatctacga tgccatccgc
     3301 aattatgtga cccagaagcc ctactctaag gataagttca agctgtattt tcagaaccct
     3361 cagttcatgg gcggctggga caaggataag gagacagact atcgggccac catcctgaga
     3421 tacggctcca agtactatct ggccatcatg gataagaagt acgccaagtg cctgcagaag
     3481 atcgacaagg acgatgtgaa cggcaattac gagaagatca actataagct gctgcccggc
     3541 cctaataaga tgctgccaaa ggtgttcttt tctaagaagt ggatggccta ctataacccc
     3601 agcgaggaca tccagaagat ctacaagaat ggcacattca agaagggcga tatgtttaac
     3661 ctgaatgact gtcacaagct gatcgacttc tttaaggata gcatctcccg gtatccaaag
     3721 tggtccaatg cctacgattt caacttttct gagacagaga agtataagga catcgccggc
     3781 ttttacagag aggtggagga gcagggctat aaggtgagct tcgagtctgc cagcaagaag
     3841 gaggtggata agctggtgga ggagggcaag ctgtatatgt tccagatcta taacaaggac
     3901 ttttccgata agtctcacgg cacacccaat ctgcacacca tgtacttcaa gctgctgttt
     3961 gacgagaaca atcacggaca gatcaggctg agcggaggag cagagctgtt catgaggcgc
     4021 gcctccctga agaaggagga gctggtggtg cacccagcca actcccctat cgccaacaag
     4081 aatccagata atcccaagaa aaccacaacc ctgtcctacg acgtgtataa ggataagagg
     4141 ttttctgagg accagtacga gctgcacatc ccaatcgcca tcaataagtg ccccaagaac
     4201 atcttcaaga tcaatacaga ggtgcgcgtg ctgctgaagc acgacgataa cccctatgtg
     4261 atcggcatcg ccaggggcga gcgcaatctg ctgtatatcg tggtggtgga cggcaagggc
     4321 aacatcgtgg agcagtattc cctgaacgag atcatcaaca acttcaacgg catcaggatc
     4381 aagacagatt accactctct gctggacaag aaggagaagg agaggttcga ggcccgccag
     4441 aactggacct ccatcgagaa tatcaaggag ctgaaggccg gctatatctc tcaggtggtg
     4501 cacaagatct gcgagctggt ggagaagtac gatgccgtga tcgccctgga ggacctgaac
     4561 tctggcttta agaatagccg cgtgaaggtg gagaagcagg tgtatcagaa gttcgagaag
     4621 atgctgatcg ataagctgaa ctacatggtg gacaagaagt ctaatccttg tgcaacaggc
     4681 ggcgccctga agggctatca gatcaccaat aagttcgaga gctttaagtc catgtctacc
     4741 cagaacggct tcatctttta catccctgcc tggctgacat ccaagatcga tccatctacc
     4801 ggctttgtga acctgctgaa aaccaagtat accagcatcg ccgattccaa gaagttcatc
     4861 agctcctttg acaggatcat gtacgtgccc gaggaggatc tgttcgagtt tgccctggac
     4921 tataagaact tctctcgcac agacgccgat tacatcaaga agtggaagct gtactcctac
     4981 ggcaaccgga tcagaatctt ccggaatcct aagaagaaca acgtgttcga ctgggaggag
     5041 gtgtgcctga ccagcgccta taaggagctg ttcaacaagt acggcatcaa ttatcagcag
     5101 ggcgatatca gagccctgct gtgcgagcag tccgacaagg ccttctactc tagctttatg
     5161 gccctgatga gcctgatgct gcagatgcgg aacagcatca caggccgcac cgacgtggat
     5221 tttctgatca gccctgtgaa gaactccgac ggcatcttct acgatagccg gaactatgag
     5281 gcccaggaga atgccatcct gccaaagaac gccgacgcca atggcgccta taacatcgcc
     5341 agaaaggtgc tgtgggccat cggccagttc aagaaggccg aggacgagaa gctggataag
     5401 gtgaagatcg ccatctctaa caaggagtgg ctggagtacg cccagaccag cgtgaagcac
     5461 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaaggg atcctaccca
     5521 tacgatgttc cagattacgc ttatccctac gacgtgcctg attatgcata cccatatgat
     5581 gtccccgact atgcctcgag cgactacaaa gaccatgacg gtgattataa agatcatgac
     5641 atcgattaca aggatgacga tgacaaggct gcaggaggcg gtggaagcgg gggaagggga
     5701 gtgcaggtgg aaaccatctc cccaggagac gggcgcacct tccccaagcg cggccagacc
     5761 tgcgtggtgc actacaccgg gatgcttgaa gatggaaaga aatttgattc ctcccgggac
     5821 agaaacaagc cctttaagtt tatgctaggc aagcaggagg tgatccgagg ctgggaagaa
     5881 ggggttgccc agatgagtgt gggtcagaga gccaaactga ctatatctcc agattatgcc
     5941 tatggtgcca ctgggcaccc aggcatcatc ccaccacatg ccactctcgt cttcgatgtg
     6001 gagcttctaa aactggaagg ttctagggga gtgcaggtgg aaaccatctc cccaggagac
     6061 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa
     6121 gatggaaaga aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc
     6181 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga
     6241 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc
     6301 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaagg gggaagcggt
     6361 ggaagcggga ggggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc
     6421 aagcgcggcc agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaattt
     6481 gattcctccc gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc
     6541 cgaggctggg aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata
     6601 tctccagatt atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact
     6661 ctcgtcttcg atgtggagct tctaaaactg gaaggttcta ggggagtgca ggtggaaacc
     6721 atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt ggtgcactac
     6781 accgggatgc ttgaagatgg aaagaaattt gattcctccc gggacagaaa caagcccttt
     6841 aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt tgcccagatg
     6901 agtgtgggtc agagagccaa actgactata tctccagatt atgcctatgg tgccactggg
     6961 cacccaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaactg
     7021 gaaggataag cggccgcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg
     7081 tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg
     7141 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
     7201 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
     7261 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
     7321 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
     7381 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     7441 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     7501 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat
     7561 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7621 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7681 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7741 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc
     7801 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7861 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7921 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
     7981 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8101 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8161 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8221 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8281 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8341 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8401 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8461 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8521 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8581 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8641 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8701 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8761 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8821 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8881 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8941 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9001 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9061 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9121 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9181 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9241 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9301 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9361 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9421 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9481 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9541 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9601 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9661 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9721 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9781 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9841 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9901 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9961 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10021 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10081 aaagtgccac ctg
//
