LOCUS       sequence-201456-       12226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector, mammalian expression of Dnd1-EGFP fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pLVCT-Dnd1-EGFP
FEATURES             Location/Qualifiers
     source          1..12226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             6..639
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    686..811
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1304..1537
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1722..1766
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2061..2440
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2442..2717
                     /label=chicken beta-actin promoter
     intron          2718..3728
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3652..3674
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3736..3755
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    3814..3932
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      5036..5045
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5042..5044,5045..5047,5048..5761)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5042..5044,5045..5047,5048..5758)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5087..5108)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5348..5367)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5695..5716
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5810..6398
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5863..5883)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6281..6292)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(6536..6554,6555..6577,6578..6596,6597..6619,6620..6638,
                     6639..6661,6662..6680,6681..6703,6704..6722,6723..6745,
                     6746..6764,6765..6787,6788..6806)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     LTR             6845..7025
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7050..7068)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7051..7068)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7358..7377)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7591..7610)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7710..7732
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7770..7788)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7856..7960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7961..8029,8030..8821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8179..8198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8992..9580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9481..9500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9734..9751
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9821..9841)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9826..10155
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10006..10141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11289..11354
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11484..11504
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11929..12063
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11966..11985)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12020..12039
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
       61 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
      121 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
      181 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
      241 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
      301 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
      361 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
      421 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
      481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
      661 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
      721 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
      781 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
      841 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
      901 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
      961 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1021 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1081 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1141 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc
     2041 ctcgaccatc gatgtcgatc gacattgatt attgactagt tattaatagt aatcaattac
     2101 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     2161 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2221 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2281 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2341 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2401 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     2461 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     2521 ttaattattt tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg
     2581 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
     2641 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
     2701 gaagcgcgcg gcgggcggga gtcgctgcgt tgccttcgcc ccgtgccccg ctccgcgccg
     2761 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
     2821 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctc gtttcttttc
     2881 tgtggctgcg tgaaagcctt aaagggctcc gggagggccc tttgtgcggg ggggagcggc
     2941 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggccc gcgctgcccg
     3001 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcgtg tgcgcgaggg
     3061 gagcgcggcc gggggcggtg ccccgcggtg cgggggggct gcgaggggaa caaaggctgc
     3121 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcggcggt cgggctgtaa
     3181 cccccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     3241 ccgtgcgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     3301 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc
     3361 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     3421 tgcgagaggg cgcagggact tcctttgtcc caaatctggc ggagccgaaa tctgggaggc
     3481 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
     3541 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
     3601 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
     3661 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
     3721 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     3781 taaatttaat taatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
     3841 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa tttaaagaat
     3901 tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgagccgc
     3961 caccatgcag tccaaacggg agtgcgagca atggtgtgag agggtgaatc cagagaacaa
     4021 ggcagccctg gaggcgtggg taagggagac tggcatccgc ctggtgcagg tcaacgggca
     4081 aaggaagtat ggagggccac ccccaggctg ggtgggcagc ccgccaccgt cagggtccga
     4141 ggtgtatatc ggacgacttc cgcaggacgt gtacgagcac cagctaatcc cactcttcca
     4201 gcgcgtgggg cgcctctatg agttccgcct aatgatgacc ttcagtggtt tgaaccgcgg
     4261 ctttgcctat gcgcgctaca gctcgcggcg cggtgcccag gccgccattg ctacgctgca
     4321 caaccaccag ctgcggcctt cttgccagct cctggtgtgc cgaagcaccg agaagtgcga
     4381 gctgacggtg gacgggctgc ctcttagctt aaaccgccga gcgttactgc tcgccctgca
     4441 accgttcggt ccctgcctcc aggagacact gctgctaccc agccctgggt ctgcgccctc
     4501 tcagattgcg ctgctcaagt tcagtacgca ccgagctgct gctatggcca aaaaggccct
     4561 ggtagaaggt cagtcacgcc tctgtggaga gcaggtggct gtggagtggc tcaagccaga
     4621 cctaaagcag cacttccgcc agcagcttgc aggtccctca ctgcggttcc tcaggccaga
     4681 tgtcagccaa ctaacccaga ccagggagaa gctggggtcc caaggggctc gggctgccct
     4741 gcaactgctg tgtcaacgga tgaagctagg cagcccagtg ttcctgacca agtgtttagg
     4801 aacagggcct gctggctggc atcgcttttg gtaccaggtg gtgatccctg ggcatccagt
     4861 gcctttcagt ggcctcattt gggttgtgtt ggcctctgat tggcaggatg ggcatgaggt
     4921 ggccaaggat gctgtgtccg cacagctgct ggaggcactg agtgaaccta ggaccagctt
     4981 gtggtctcct ggggctgaag caggtactat ggttaagcag aaggatccac cggtcgccac
     5041 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     5101 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     5161 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     5221 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     5281 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     5341 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5401 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     5461 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     5521 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     5581 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     5641 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     5701 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     5761 aagcggccag cttatcgact cgcgaattcg atgggactag tcatatgata atcaacctct
     5821 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     5881 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     5941 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6001 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6061 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6121 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6181 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6241 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6301 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6361 tcagacgagt cggatctccc tttgggccgc ctccccgcat cggtacgtat ggccaggtac
     6421 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6481 ggggactgga agggctaatt cactcccaac gaagacaaga tctcgagttt accactccct
     6541 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     6601 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     6661 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     6721 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     6781 ttaccactcc ctatcagtga tagagaaaag tgaaagtcgg atcgatctgc tttttgcttg
     6841 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6901 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6961 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7021 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg
     7081 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat
     7141 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga
     7201 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg
     7261 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg
     7321 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     7381 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     7441 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     7501 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     7561 aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     7621 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     7681 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     7741 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     7801 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7861 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7921 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7981 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8041 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8101 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8161 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8221 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8281 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8341 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8401 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8461 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8521 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8581 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8641 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8701 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8761 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8821 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8881 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8941 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9001 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9061 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9121 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9181 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9241 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9301 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     9361 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
     9421 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9481 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9541 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9601 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9661 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9721 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     9781 gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg
     9841 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9901 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9961 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    10021 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    10081 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    10141 ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc
    10201 actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact
    10261 ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt
    10321 ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg
    10381 ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg
    10441 gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa
    10501 ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc
    10561 gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac
    10621 ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt
    10681 gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc
    10741 ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc
    10801 tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg
    10861 cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct
    10921 gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg
    10981 ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca
    11041 ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat
    11101 cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata
    11161 cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg
    11221 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
    11281 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
    11341 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
    11401 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
    11461 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
    11521 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
    11581 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
    11641 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
    11701 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
    11761 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
    11821 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
    11881 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
    11941 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    12001 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    12061 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
    12121 accactgcca attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    12181 ttcattttaa agaaattgta tttgttaaat atgtactaca aactta
//
