LOCUS       sequence-201460-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used as a PCR template to amplify the M9-HA point mutant of 
            glmS fragment for gene specific tagging that only allows tagging but
            not regulation. Acts as a control for glmS based gene regulation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Cruz-Bustos T, Ramakrishnan S, Cordeiro CD, Ahmed MA, Docampo R
  TITLE     A Riboswitch-based Inducible Gene Expression System for Trypanosoma 
            brucei.
  JOURNAL   J Eukaryot Microbiol. 2017 Dec 19. doi: 10.1111/jeu.12493.
  PUBMED    29265590
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Eukaryot 
            Microbiol. 2017 Dec 19. doi: 10.1111/jeu.12493."
COMMENT     Sequence Label: pMOTag-M9-4H
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..84
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 gagtaccctt acgatgtgcc tgattacgcg tacccatacg acgtgccaga ctacgcatac
       61 ccgtacgatg tgcccgatta cgcatagtcg actgagtaat tatcccgccc gaactaagcg
      121 cccggaaaaa ggcttagttg acgaggatgg aggttatcga attttcggcg gatgcctccc
      181 ggctgagtgt gcagatcaca gccgtaagga tttcttcaaa ccaagggggt gactccttga
      241 acaaagagaa atcacatgat ctgtcgacaa agtgtgacaa cgtcgcacca tgtgtaggtt
      301 ttcatttatg ttctttcttt ctttcttttg tgaatttgtt ttctgtctca aatgttttta
      361 attcgcttgg gacctatgtt tttcttgttt ttttgctcac cctttgtgta ggaggcaccc
      421 tgtcacgtct gtggttgcgt gtatgccttc cttcccctta ttcgcttctt cctgtcgtgt
      481 cacacctctt tctccctctc cctttccgcc ttttctttca atcttgtttt ctcgaccagc
      541 cctactagag gagaaagaat agtaaccctt tcatcaaaga aaatagttca aacgaattgc
      601 tagcttaagc ttgatctaga actagtgatg aaaaagcctg aactcaccgc gacgtctgtc
      661 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
      721 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
      781 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
      841 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
      901 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg anncgaactg cccgctgttc
      961 tgcagccggt cgcggaggcc atggntgcga tcgctgcggc cgatcttanc cagac
//
