LOCUS       sequence-201477-       10238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses GLI2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10238)
  TITLE     human Gli2 full length
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GLI2 his
FEATURES             Location/Qualifiers
     source          1..10238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             932..949
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             953..985
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(989..997,998..1012)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(5829..5846)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5835..6059
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6105..6533
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6192..6211)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6402..6423
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6542..6562)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6547..6876
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6727..6862
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6789..6808
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6943..7737
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6997..7016)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7607..7626
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7911..8032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7948..7967)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8002..8021
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8081..8097)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8081..8097)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8094..8116)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8105..8121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8129..8135,8136..8153,8154..8159))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8174..8195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8312..8329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8483..9071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8563..8582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9242..10033,10034..10102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9865..9884
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10103..10207)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttacca tggggggttc tcatcatcat catcatcatg gtatggctag
      961 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggtaccagg
     1021 atccagtgtg gtggaattca tggagacgtc tgcctcagcc actgcctccg agaagcaaga
     1081 agccaaaagt gggatcctgg aggccgctgg cttccccgac ccgggtaaaa aggcctctcc
     1141 tttggtggtg gctgcagcgg cagcagcagc ggtagctgcc caaggagtgc cgcagcatct
     1201 cttgccacca ttccatgcgc ccctaccgat tgacatgcga caccaggaag gaaggtacca
     1261 ttacgagcct cattctgtcc acggtgtgca cgggccccct gccctcagcg gcagccctgt
     1321 catctctgac atctccttga tccggctttc cccgcacccg gctggccctg gggagtcccc
     1381 cttcaacgcc ccccacccgt acgtgaaccc ccacatggag cactacctcc gttctgtgca
     1441 cagcagcccc acgctctcca tgatctctgc agccaggggc ctcagccccg ctgatgtggc
     1501 ccaggagcac cttaaggaga ggggactgtt tggccttcct gctccaggca ccaccccctc
     1561 agactattac caccagatga ccctcgtggc aggccacccc gcgccctacg gggacctgct
     1621 gatgcagagc gggggcgctg ccagcgcacc ccatctccac gactacctca accccgtgga
     1681 cgtgtcccgt ttctccagcc cgcgggtgac gccccgcctg agccgcaagc gggcgctgtc
     1741 catctcccca ctctcagacg ccagcctgga cctgcagcgg atgatccgca cctcacccaa
     1801 ctcgctagtg gcctacatca acaactcccg aagcagctca gcggccagcg gttcctacgg
     1861 gcatctgtca gcgggtgccc tcagcccagc cttcaccttc ccccacccca tcaaccccgt
     1921 ggcctaccag cagattctga gccagcagag gggtctgggg tcagcctttg gacacacacc
     1981 acccctgatc cagccctcac ccaccttcct ggcccagcag cccatggccc tcacctccat
     2041 caatgccacg cccacccagc tcagcagcag cagcaactgt ctgagtgaca ccaaccagaa
     2101 caagcagagc agtgagtcgg ccgtcagcag caccgtcaac cctgtcgcca ttcacaagcg
     2161 cagcaaggtc aagaccgagc ctgagggcct gcggccggcc tcccctctgg cgctgacgca
     2221 gggccaggtg tctggacacg gctcatgtgg gtgtgccctt cccctctccc aggagcagct
     2281 ggctgacctc aaggaagatc tggacaggga tgactgtaag caggaggctg aggtggtcat
     2341 ctatgagacc aactgccact gggaagactg caccaaggag tacgacaccc aggagcagct
     2401 ggtgcatcac atcaacaacg agcacatcca cggggagaag aaggagtttg tgtgccgctg
     2461 gcaggcctgc acgcgggagc agaagccctt caaggcgcag tacatgctgg tggtgcacat
     2521 gcggcgacac acgggcgaga agccccacaa gtgcacgttc gagggctgct cgaaggccta
     2581 ctcccgcctg gagaacctga agacacacct gcggtcccac accggggaga agccatatgt
     2641 gtgtgagcac gagggctgca acaaagcctt ctccaacgcc tcggaccgcg ccaagcacca
     2701 gaatcgcacc cactccaacg agaaacccta catctgcaag atcccaggct gcaccaagag
     2761 atacacagac cccagctctc tccggaagca tgtgaaaacg gtccacggcc cagatgccca
     2821 cgtcaccaag aagcagcgca atgacgtgca cctccgcaca ccgctgctca aagagaatgg
     2881 ggacagtgag gccggcacgg agcctggcgg cccagagagc accgaggcca gcagcaccag
     2941 ccaggccgtg gaggactgcc tgcacgtcag agccatcaag accgagagct ccgggctgtg
     3001 tcagtccagc cccggggccc agtcgtcctg cagcagcgag ccctctcctc tgggcagtgc
     3061 ccccaacaat gacagtggcg tggagatgcc ggggacgggg cccgggagcc tgggagacct
     3121 gacggcactg gatgacacac ccccaggggc cgacacctca gccctggctg ccccctccgc
     3181 tggtggcctc cagctgcgca aacacatgac caccatgcac cggttcgagc agctcaagaa
     3241 ggagaagctc aagtcactca aggattcctg ctcatgggcc gggccgactc cacacacgcg
     3301 gaacaccaag ctgcctcccc tcccgggaag tggctccatc ctggaaaact tcagtggcag
     3361 tgggggcggc gggcccgcgg ggctgctgcc gaacccgcgg ctgtcggagc tgtccgcgag
     3421 cgaggtgacc atgctgagcc agctgcagga gcgccgcgac agctccacca gcacggtcag
     3481 ctcggcctac accgtgagcc gccgctcctc cggcatctcc ccctacttct ccagccgccg
     3541 ctccagcgag gcctcgcccc tgggcgccgg ccgcccgcac aacgcgagct ccgctgactc
     3601 ctacgacccc atctccacgg acgcgtcgcg gcgctcgagc gaggccagcc agtgcagcgg
     3661 cggctccggg ctgctcaacc tcacgccggc gcagcagtac agcctgcggg ccaagtacgc
     3721 ggcagccact ggcggccccc cgcccactcc gctgccgggc ctggagcgca tgagcctgcg
     3781 gaccaggctg gcgctgctgg acgcgcccga gcgcacgctg cccgccggct gcccacgccc
     3841 actggggccg cggcgtggca gcgacgggcc gacctatggc cacggccacg cgggggctgc
     3901 gcccgccttc ccccacgagg ctccaggcgg cggagccagg cgggccagcg accctgtgcg
     3961 gcggcccgat gccctgtccc tgccgcgggt gcagcgcttc cacagcaccc acaacgtgaa
     4021 ccccggcccg ctgccgccct gtgccgacag gcgaggcctc cgcctgcaga gccacccgag
     4081 caccgacggc ggcctggccc gcggcgccta ctcgccccgg ccgcctagca tcagcgagaa
     4141 cgtggcgatg gaggccgtgg cggcaggagt ggacggcgcg gggcccgagg ccgacctggg
     4201 gctgccggag gacgacctgg tgcttccaga cgacgtggtg cagtacatca aggcgcacgc
     4261 cagtggcgct ctggacgagg gcaccgggca ggtgtatccc acggaaagca ctggcttctc
     4321 tgacaacccc agactaccca gcccggggct gcacggccag cgcaggatgg tggctgcgga
     4381 ctccaacgtg ggcccctccg cccctatgct gggaggatgc cagttaggct ttggggcgcc
     4441 ctccagcctg aacaaaaata acatgcctgt gcagtggaat gaggtgagct ccggcaccgt
     4501 agacgccctg gccagccagg tgaagcctcc accctttcct cagggcaacc tggcggtggt
     4561 gcagcagaag cctgcctttg gccagtaccc gggctacagt ccgcaaggcc tacaggctag
     4621 ccctgggggc ctggacagca cgcagccaca cctgcagccc cgcagcggag ccccctccca
     4681 gggcatcccc agggtaaact acatgcagca gctgcgacag ccagtggcag gcagccagtg
     4741 tcctggcatg actaccacta tgagccccca tgcctgctat ggccaagtcc acccccagct
     4801 gagccccagc accatcagtg gggccctcaa ccagttcccc caatcctgca gcaacatgcc
     4861 agccaagcca gggcatctgg ggcaccctca gcagacagaa gtggcacctg accccaccac
     4921 gatgggcaat cgccacaggg aacttggggt ccccgattca gccctggctg gagtgccacc
     4981 acctcaccca gtccagagct acccacagca gagccatcac ctggcagcct ccatgagcca
     5041 ggagggctac caccaggtcc ccagccttct gcctgcccgc cagcctggct tcatggagcc
     5101 ccaaacaggc ccgatggggg tggctacagc aggctttggc ctagtgcagc cccggcctcc
     5161 cctcgagccc agccccactg gccgccaccg tggggtacgt gctgtgcagc agcagctggc
     5221 ctacgccagg gccacaggcc atgccatggc tgccatgccg tccagtcagg aaacagcaga
     5281 ggctgtgccc aagggagcga tgggcaacat ggggtcggtg cctccccagc cgcctccgca
     5341 ggacgcaggt ggggccccgg accacagcat gctctactac tacggccaga tccacatgta
     5401 cgaacaggat ggaggcctgg agaacctcgg gagctgccag gtcatgcggt cccagccacc
     5461 acagccacag gcctgtcagg acagcatcca gccccagccc ttgccctcac caggggtcaa
     5521 ccaggtgtcc agcactgtgg actcccagct cctggaggcc ccccagattg acttcgatgc
     5581 catcatggat gatggcgatc actcgagttt gttctcgggt gctctgagcc ccagcctcct
     5641 ccacagcctc tcccagaact cctcccgcct caccaccccc cgaaactcct tgaccctgcc
     5701 ctccatcccc gcaggcatca gcaacatggc tgtcggggac atgagctcca tgctcaccag
     5761 cctcgccgag gagagcaagt tcctgaacat gatgacctct agagggcccg tttaaacccg
     5821 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     5881 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     5941 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     6001 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     6061 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     6121 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6181 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6241 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6301 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6361 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6421 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6481 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     6541 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6601 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     6661 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     6721 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6781 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     6841 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     6901 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     6961 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     7021 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     7081 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     7141 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     7201 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     7261 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     7321 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     7381 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     7441 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     7501 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     7561 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     7621 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     7681 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     7741 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     7801 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7861 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7921 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7981 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8041 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     8101 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8161 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8221 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     8281 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8341 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8401 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8461 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8521 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8581 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8641 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8701 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8761 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8821 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8881 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8941 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9001 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9061 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9121 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9181 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9241 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9301 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9361 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9421 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9481 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9541 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9601 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9661 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9721 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9781 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9841 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9901 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9961 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10021 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10081 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10141 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10201 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
