LOCUS       sequence-201479-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses catalytically inactive dCas9 with N-terminal fusion of 
            Sin3a Interacting Domain (SID) and C-terminal fusion of repressive 
            KRAB domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Carleton JB, Berrett KC, Gertz J
  TITLE     Multiplex Enhancer Interference Reveals Collaborative Control of 
            Gene Regulation by Estrogen Receptor alpha-Bound Enhancers.
  JOURNAL   Cell Syst. 2017 Oct 25;5(4):333-344.e5. doi: 
            10.1016/j.cels.2017.08.011. Epub 2017 Sep 27.
  PUBMED    28964699
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2017.08.011"; journalName: "Cell Syst"; date: 
            "2017-10-25- 25"; volume: "5"; issue: "4"; pages: "333-344.e5"
COMMENT     Sequence Label: SID4x-dCas9-KRAB
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    76..100
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      115..124
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             520..546
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             550..570
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
       61 gggaaaaaac aattgacaag tttgtacaaa aaagcaggct ccgaattcac cggtgccacc
      121 atgggctccg gaatgaacat tcagatgttg ctcgaggctg ctgattacct ggagcgccgc
      181 gaacgggaag cggaacacgg ttacgctagt atgctgcctg ggagcggcat gaatatccag
      241 atgctgctcg aggcggccga ttatctcgaa cgcagagaga gagaagccga acatggctac
      301 gcctctatgc tgcctggctc cgggatgaat atacagatgc tcctggaagc tgccgactat
      361 ctggagcgaa gagaaaggga ggctgagcac gggtatgcaa gtatgctccc tggctccggt
      421 atgaatatcc agatgctgct ggaggccgct gactatcttg aacgccgaga aagggaagcc
      481 gagcatggat atgcatccat gctgccttct aggtctgcct acccatacga tgttccagat
      541 tacgcttcgc cgaagaaaaa gcgcaaggtc gaagcgtccg acaagaagta cagcatcggc
      601 ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
      661 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
      721 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
      781 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
      841 gagatggccn angtggacga cagcttcttc cacagactgn aagagtcctt cctggtgg
//
