LOCUS       sequence-201480-         321 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses catalytically inactive dCas9 with N-terminal fusion of 
            Sin3a Interacting Domain (SID) and C-terminal fusion of repressive 
            KRAB domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Carleton JB, Berrett KC, Gertz J
  TITLE     Multiplex Enhancer Interference Reveals Collaborative Control of 
            Gene Regulation by Estrogen Receptor alpha-Bound Enhancers.
  JOURNAL   Cell Syst. 2017 Oct 25;5(4):333-344.e5. doi: 
            10.1016/j.cels.2017.08.011. Epub 2017 Sep 27.
  PUBMED    28964699
REFERENCE   2  (bases 1 to 321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2017.08.011"; journalName: "Cell Syst"; date: 
            "2017-10-25- 25"; volume: "5"; issue: "4"; pages: "333-344.e5"
COMMENT     Sequence Label: SID4x-dCas9-KRAB
FEATURES             Location/Qualifiers
     source          1..321
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..212
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             213..278
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cacttacggc atggtcgaga acactggtta cgttcaagga cgtgtttgtg gactttacac
       61 gtgaggagtg gaaattgctg gatactgcgc aacaaattgt gtatcgaaat gtcatgcttg
      121 agaattacaa gaacctcgtc agtctcggat accagttgac gaaaccggat gtgatcctta
      181 ggctcgaaaa gggggaagaa ccttggctgg tagactacaa agaccatgac ggtgattata
      241 aagatcatga catcgactac aaggatgacg atgacaaaat cgatagatct ggcagcggag
      301 agggcagagg aagccttcta a
//
