LOCUS       sequence-201484-        9556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual-intron DMD platform plasmid. Co-expresses DMD platform segment,
            firefly luciferase, puroR and mCherry. Plasmid carries phiC31 and 
            Bxb1 attB sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9556)
  AUTHORS   Farruggio AP, Bhakta MS, du Bois H, Ma J, P Calos M
  TITLE     Genomic integration of the full-length dystrophin coding sequence in
            Duchenne muscular dystrophy induced pluripotent stem cells.
  JOURNAL   Biotechnol J. 2017 Jan 31. doi: 10.1002/biot.201600477.
  PUBMED    28139886
REFERENCE   2  (bases 1 to 9556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2017 Jan 31. doi: 10.1002/biot.201600477."
COMMENT     Sequence Label: p28-2iDP-puro
FEATURES             Location/Qualifiers
     source          1..9556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    43..112
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     enhancer        402..705
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        707..984
                     /label=chicken beta-actin promoter
     intron          985..2000
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1924..1946
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2008..2027
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3307..3363
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3364..5013
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3430..3448)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             5023..5079
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5686..5742
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5743..6453
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5888..5906)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6156..6175
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6460..7048
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6513..6533)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6931..6942)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7063..7080)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7069..7293
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7435..7484
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     CDS             7803..8597
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7803..8597
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7857..7876)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8467..8486
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      8922..9510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9411..9430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg
       61 gtgccagggc gtgcccttgg gctccccggg cgcgtactcc acctcaccca tctggtccat
      121 catgatgaac gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa
      181 gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc
      241 gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc tcgacggtca cggcgggcat
      301 gtcgacggta tcgatccata gagcccaccg catccccagc atgcttcata atagtaatca
      361 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      421 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      481 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      541 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      601 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      661 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc
      721 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt
      781 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcg cgcgccaggc
      841 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat
      901 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
      961 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc
     1021 tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     1081 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
     1141 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
     1201 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
     1261 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
     1321 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
     1381 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
     1441 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     1501 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     1561 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc
     1621 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     1681 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     1741 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     1801 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     1861 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     1921 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     1981 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     2041 ttggcaaaga attccgccac catgctttgg tgggaagaag tagaggactg ttatgaaaga
     2101 gaagatgttc aaaagaaaac attcacaaaa tgggtaaatg cacaattttc taagtttggg
     2161 aagcagcata ttgagaacct cttcagtgac ctacaggatg ggaggcgcct cctagacctc
     2221 ctcgaaggcc tgacagggca aaaactggta tgtatcaagc ttacaagaca gctttaagga
     2281 gaccaataga aactggggca tgtggagaca gagaagactc ttgggcctcg aggcttcatt
     2341 ttttcttttt ctttttcagc caaaagaaaa aggatccaca agagttcatg ccctgaacaa
     2401 tgtcaacaag gcactgcggg ttttgcagaa caataatgtt gatttagtga atattggaag
     2461 tactgacatc gtagatggaa atcataaact gactcttggt ttgatttgga atataatcct
     2521 ccactggcag gtcaaaaatg taatgaaaaa tatcatggct ggattgcaac aaaccaacag
     2581 tgaaaagatt ctcctgagct gggtccgaca atcaactcgt aattatccac aggttaatgt
     2641 aatcaacttc accaccagct ggtctgatgg cctggctttg aatgctctca tccatagtca
     2701 taggccagac ctatttgact ggaatagtgt ggtttgccag cagtcagcca cacaacgact
     2761 ggaacatgca ttttcttccc tgtgtttgtt ctccaccttt tcgtctctcc gggatgctga
     2821 gctcattgct gaggccaagc tactgcgtca acacaaaggc cgcctggaag ccaggatgca
     2881 aatcctggaa gaccacaata aacagctgga gtcacagtta cacaggctaa ggcagctgct
     2941 ggagcaaccc caggcagagg ccaaagtgaa tggcacaacg gtgtcctctc cttctacctc
     3001 tctacagagg tccgacagca gtcagcctat gctgctccga gtggttggca gtcaaacttc
     3061 ggactccatg ggtaagtaga gggctgggct gggctgtggg gggtgtgggg tgcgggactg
     3121 ggcagtctgg gagtccctct caccactttt cttacctttc taggtgagga agatcttctc
     3181 agtcctcccc aggacacaag cacagggtta gaggaggtga tggagcaact caacaactcc
     3241 ttccctagtt caagaggaag aaatacccct ggaaagccaa tgagagagga cacaatggga
     3301 tccggtgcca cgaacttctc tctgttaaag caagcaggag atgtggaaga aaaccccggt
     3361 cctatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat
     3421 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
     3481 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg
     3541 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
     3601 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
     3661 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc
     3721 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
     3781 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag
     3841 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac
     3901 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct
     3961 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc
     4021 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
     4081 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
     4141 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat
     4201 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact
     4261 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc
     4321 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga
     4381 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg
     4441 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg
     4501 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc
     4561 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
     4621 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag
     4681 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc
     4741 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa
     4801 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
     4861 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
     4921 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc
     4981 ataaaggcca agaagggcgg aaagatcgcc gtgggatccg gtgccacgaa cttctctctg
     5041 ttaaagcaag caggagatgt ggaagaaaac cccggtccta tgactgaata caaaccaact
     5101 gttcgcctgg caactcgtga tgatgttcca cgtgcagttc gcaccctggc tgctgcattt
     5161 gctgactacc ctgcaacccg tcacactgtg gacccagacc gccacattga acgtgtgact
     5221 gaactgcagg agctgttcct gacccgtgtg ggcctggaca ttggcaaagt gtgggtggca
     5281 gatgatggtg ctgctgtggc agtgtggacc acccctgaat ctgttgaagc tggtgcagtg
     5341 tttgctgaga ttggcccacg catggcagaa ctgtctggca gccgcctggc agcacaacag
     5401 cagatggaag gtctgctggc accacaccgc ccaaaagaac ctgcttggtt cctggcaact
     5461 gtgggtgtga gccctgacca ccagggtaag ggcctgggct ctgcagtggt gctgcctggt
     5521 gtggaagcag ctgaacgtgc aggtgtgcct gctttcctgg agacctcagc tccacgcaac
     5581 ctgcctttct atgaacgcct gggcttcact gtgactgctg atgtggaagt gccagaaggc
     5641 ccacgcactt ggtgcatgac tcgcaaacca ggtgctggat ccggtgccac gaacttctct
     5701 ctgttaaagc aagcaggaga tgtggaagaa aaccccggtc ctatggtgag caagggcgag
     5761 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     5821 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     5881 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     5941 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     6001 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     6061 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     6121 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     6181 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     6241 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     6301 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     6361 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     6421 tccaccggcg gcatggacga gctgtacaag taaatcgata atcaacctct ggattacaaa
     6481 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6541 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6601 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6661 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6721 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6781 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6841 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     6901 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6961 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7021 cggatctccc tttgggccgc ctccccgcat cgatactcga gccctcgact gtgccttcta
     7081 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     7141 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     7201 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     7261 gcaggcatgc tggggatgcg gtgggctcta tggggcgcgc gaaaccggtg aaaaaccgct
     7321 cagcggcgcc cgcacccaaa cgaaaacacc cccggcccaa gcggaccggg ggtgtcgcgt
     7381 gaaatggatc agtgagcgtg gccgtggtgg tggccgtggc cgtgctcgtc ctcgtcggcc
     7441 ggcttgtcga cgacggcggt ctccgtcgtc aggatcatcc gggccaccga ggcggcgttg
     7501 agaacagccg agcgggtcac cttggccgga tcgacgacgc cggcggagac cagatcaccg
     7561 aactccagcg tggcggcgtt gaagccctgc cccttgggaa gttcggagat taattaaccg
     7621 tttaaattgg gcgcgccaag gaagctagca ggacagcaag cgaaccggaa ttgccagctg
     7681 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     7741 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc
     7801 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     7861 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     7921 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     7981 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     8041 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     8101 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     8161 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     8221 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     8281 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     8341 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     8401 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     8461 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     8521 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     8581 ttgacgagtt cttctgaatt attaacgctt acaatttcct gatgcggtat tttctcctta
     8641 cgcatctgtg cggtatttca caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga
     8701 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8761 ccctgataaa tgcttcaata atagcacgtg ctaaaacttc atttttaatt taaaaggatc
     8821 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     8881 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     8941 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9001 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9061 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9121 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9181 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9241 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9301 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9361 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9421 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9481 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9541 ctgggctttt gctggc
//
