LOCUS       sequence-201491-       12962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of Psd95 shRNA, and EGFP-SAP97beta fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12962)
  AUTHORS   Schluter OM, Xu W, Malenka RC
  TITLE     Alternative N-terminal domains of PSD-95 and SAP97 govern 
            activity-dependent regulation of synaptic AMPA receptor function.
  JOURNAL   Neuron. 2006 Jul 6;51(1):99-111.
  PUBMED    16815335
REFERENCE   2  (bases 1 to 12962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2006-07-6"; volume: "51(1)"; pages: "99-111"
COMMENT     Sequence Label: FH95pUG+p97W (B8)
FEATURES             Location/Qualifiers
     source          1..12962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2610..2824
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     2745..2764
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        2928..4139
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3976..3995
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4185..4187,4188..4190,4191..4901)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4230..4251)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4491..4510)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4838..4859
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7660..8248
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7713..7733)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8131..8142)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8767..8947
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8973..8990)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8979..9203
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9249..9677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9336..9355)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9546..9567
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9686..9706)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9691..10020
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9871..10006
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9933..9952
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10068..10115
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10134..10508
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10638..10759
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10675..10694)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10729..10748
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10808..10824)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10808..10824)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10821..10843)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10832..10848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10856..10862,10863..10880,10881..10886))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10901..10922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11039..11056)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11210..11798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11290..11309)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11969..12760,12761..12829))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12592..12611
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12830..12934)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcg tacgaattcg aacgctgacg tcatcaaccc gctccaagga
     2641 atcgcgggcc cagtgtcact aggcgggaac acccagcgcg cgtgcgccct ggcaggaaga
     2701 tggctgtgag ggacagggga gtggcgccct gcaatatttg catgtcgcta tgtgttctgg
     2761 gaaatcacca taaacgtgaa atgtctttgg atttgggaat cttataagtt ctgtatgaga
     2821 ccacagatcc ccggacatcc aggcacacaa gttcaagaga cttgtgtgcc tggatgtcct
     2881 ttttggaaag cttatcgaat taattaaccc gtgtcggctc cagatctggc ctccgcgccg
     2941 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     3001 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     3061 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     3121 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     3181 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     3241 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     3301 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     3361 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     3421 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     3481 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     3541 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     3601 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     3661 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     3721 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     3781 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     3841 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     3901 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     3961 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     4021 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     4081 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     4141 aagcttgggc tgcaggtcga ctctagagga tccaccggtc caccatggtg agcaagggcg
     4201 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     4261 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     4321 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     4381 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     4441 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     4501 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     4561 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     4621 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggcgaact
     4681 tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga
     4741 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     4801 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     4861 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag
     4921 ctcaagcttc gaattctatg ccggtccgga agcaagatac ccagagagca ttgcatctgt
     4981 tggaagaata tcggtcgaaa ctaagccaaa ccgaagacag acagctcagg agttccatag
     5041 agcgggttat tagcatattt cagagcaacc tctttcaggc tttaatagac attcaagaat
     5101 tttatgaagt gaccttactt gataatccaa aatgtgtgga tcattcaaag cagtgtgaac
     5161 cggtccaacc cgggaatcct tgggagagtg gcagcctttc aagtgctgct gtgacttcag
     5221 agagcctgcc cggcggcctt agccctccag tagagaaata ccggtatcag gacgaagagg
     5281 tgctgccttc agagcggatt tccccgcaag tcccaaatga ggtgctgggt ccggagctgg
     5341 ttcacgtctc ggagaagagc ctgtcagaga ttgagaatgt ccacgggttt gtctctcact
     5401 ctcatatctc acccataaag gcaaatcctc ctcctgtgct ggtcaacaca gacagcttgg
     5461 agacaccaac ttatgttaat ggtactgatg cagattatga atatgaggaa atcacacttg
     5521 aaaggggaaa ttcaggcctt ggtttcagca ttgcaggagg tacagacaac ccacacattg
     5581 gagatgactc aagtattttc atcaccaaaa ttatcacggg aggagcagct gcccaggatg
     5641 gaagattgcg ggtaaatgac tgtatactga gagtaaatga agcagatgtt cgtgatgtaa
     5701 cccacagcaa agcagttgaa gcattgaaag aagctggatc gatagtgcga ttatatgtga
     5761 aaaggcggaa gccagcctca gaaaaaatca tggaaataaa acttattaaa ggccctaaag
     5821 gtcttgggtt cagcattgct ggaggtgttg gaaaccagca cattcctggg gataacagca
     5881 tctatgtaac caaaataatt gaaggaggtg cagcacacaa ggacggcaaa cttcagattg
     5941 gagacaagct tctagcagtg aacagtgtct gtttagaaga agttactcat gaagaagcag
     6001 tgactgcctt aaagaataca tctgattttg tttatttgaa agtggcaaaa ccaacaagta
     6061 tgtatataaa tgatggctat gcaccacccg acatcactaa ttcttcttct cagtctgttg
     6121 acaaccatgt tagcccgtct tcatacttgg gccagactcc agcgtcacca gccagatact
     6181 cacccatttc taaagcagtg cttggagatg atgagatcac tagggaacct agaaaagttg
     6241 ttcttcatcg tggctcaacg ggacttggtt tcaacattgt gggaggcgaa gatggagaag
     6301 ggatttttat ctctttcatc cttgctgggg gacctgctga cctaagcgga gagctcagaa
     6361 aaggagatcg cattatatcg gtaaacagtg ttgacctcag agctgcaagt catgaacaag
     6421 cagctgccgc attaaaaaac gcaggccaag ctgtcactat tgtcgcacag tatcggcctg
     6481 aagagtacag tcgttttgaa gctaaaattc acgacttgcg ggagcagatg atgaacagca
     6541 gtgtcagctc agggtcaggg tctctccgaa ccagccaaaa gcggtccctc tatgtcagag
     6601 ccctttttga ttacgacaag actaaagaca gtggccttcc cagtcaaggg ttgaacttca
     6661 aattcggaga tattctccat gttatcaatg cttctgatga cgagtggtgg caggccagac
     6721 aggtgacccc agatggtgag agtgacgaag ttggagtaat tcctagtaaa cgcagagttg
     6781 agaagaaaga acgagcccga ttaaaaacag tcaaattcaa ttctaaaaca agaggagata
     6841 aagggtcatt caatgacaag cgtaaaaaga acctcttttc ccgaaaattt cccttctaca
     6901 agaacaagga ccagagtgag caggaaacaa gtgatgctga ccagcacgta acttctaatg
     6961 ccagcgatag cgaaagtagt taccgtggtc aagaagaata tgttttatcg tatgagccgg
     7021 tgaatcagca agaagttaat tatacccgac cagtcatcat attaggacct atgaaagaca
     7081 gagtaaatga tgacttaatc tcagaatttc ctgacaaatt tggatcctgt gtccctcata
     7141 caactagacc aaagcgtgac tatgaggtgg atggacgaga ttaccatttt gtgacttcaa
     7201 gagaacagat ggagaaagat atccaggagc ataaattcat tgaagctggc cagtataaca
     7261 accatctata cggaacgagt gtccaatctg tgcgagcagt agcagagaag ggcaagcatt
     7321 gtatccttga tgtgtctgga aatgccatca agaggttgca gattgcacag ctctatccaa
     7381 tatctatttt tattaaaccc aaatccatgg aaaatatcat ggaaatgaat aaacgcctaa
     7441 cagacgaaca ggccagaaaa acgtttgaga gagcagtgag actggagcag gagttcactg
     7501 agcatttcac agctattgtc cagggagaca cgctggagga catttacaat caagtgaagc
     7561 agatcataga agagcagtct ggtccttaca tctgggtccc agcaaaagaa aaattatgag
     7621 agctcgagct agaattaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     7681 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7741 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7801 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7861 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7921 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7981 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     8041 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     8101 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     8161 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     8221 cggatctccc tttgggccgc ctccccgcat cgataccgtc gagacctaga aaaacatgga
     8281 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     8341 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     8401 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     8461 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     8521 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     8581 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     8641 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     8701 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     8761 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8821 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8881 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8941 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     9001 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     9061 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     9121 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     9181 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     9241 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     9301 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     9361 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     9421 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     9481 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     9541 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     9601 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     9661 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     9721 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     9781 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9841 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     9901 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     9961 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
    10021 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
    10081 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
    10141 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
    10201 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
    10261 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
    10321 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
    10381 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
    10441 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
    10501 aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
    10561 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
    10621 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    10681 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10741 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
    10801 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    10861 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    10921 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    10981 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    11041 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    11101 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    11161 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    11221 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    11281 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    11341 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    11401 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    11461 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    11521 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    11581 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    11641 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    11701 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    11761 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    11821 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    11881 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    11941 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    12001 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    12061 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    12121 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    12181 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    12241 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    12301 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    12361 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    12421 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    12481 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    12541 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    12601 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    12661 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    12721 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    12781 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    12841 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    12901 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    12961 ac
//
