LOCUS       sequence-201511-         240 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses trans SNARE complex with pDC183 in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Zhou Q, Zhou P, Wang AL, Wu D, Zhao M, Sudhof TC, Brunger AT
  TITLE     The primed SNARE-complexin-synaptotagmin complex for neuronal 
            exocytosis.
  JOURNAL   Nature. 2017 Aug 24;548(7668):420-425. doi: 10.1038/nature23484. 
            Epub 2017 Aug 16.
  PUBMED    28813412
REFERENCE   2  (bases 1 to 240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature23484"; journalName: "Nature"; date: "2017-08-24- 
            24"; volume: "548"; issue: "7668"; pages: "420-425"
COMMENT     Sequence Label: pQZ210
FEATURES             Location/Qualifiers
     source          1..240
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..30
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             31..51
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 catcaccatc accatcacca tcaccatcac gaaaacctgt attttcaggg cagcaacagg
       61 agactgcagc agacccaggc ccaggtggat gaggtggtgg acatcatgag ggtgaatgtg
      121 gacaaggtcc tggagcgaga ccagaagcta tcggaactgg atgatcgcta agatgccctc
      181 caggcagggg cctcccagtt tgaaacaagt gcagccaagc tcaagcgcaa atactggtag
//
