LOCUS       sequence-20152-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Logan M, Martin JF, Nagy A, Lobe C, Olson EN, Tabin CJ
  TITLE     Expression of Cre Recombinase in the developing mouse limb bud 
            driven by a Prxl enhancer.
  JOURNAL   Genesis. 2002 Jun . 33(2):77-80.
  PUBMED    12112875
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2002 Jun . 33(2):77-80."
COMMENT     Sequence Label: pBS mPrx1 (CT#640)
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8..24
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     30..49
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(358..378)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          508..573
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        657..675
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     657..674
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cccccctcga ggtcgacggt atcgattgtg aaatttgtga tgctattgct ttatttgtaa
       61 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      121 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
      181 ctgattatga tctctagtca aggcactata catcaaatat ttttccataa ttttcttgta
      241 tagcagtgca gctttttcct ttgtggtgta aatagcaaag caagcaagag ttctattact
      301 aaacacagca tgactcaaaa aacttagcaa ttctgaagga aagtccttgg ggtcttctac
      361 ctttctcttc ttttttggag gagtagaatg ttgagagtca gcagtagcct catcatcact
      421 agatggcatt tcttctgagc aaaacaggtt ttcctcatta aaggcattcc accactgctc
      481 ccattcatca gttccatagg ttggaatcta aaatacacaa acaattagaa tcagtagttt
      541 aacacattat acacttaaaa attttatatt taccttagag ctttaaatct ctgtaggtag
      601 tttgtccaat tatgtcacac cacagaagta aggttccttc acaaagatcc tctagcattt
      661 aggtgacact atagaatagg gccctcaagc ttgatatcga attcctgcag cccgggggat
      721 ctccact
//
