LOCUS       sequence-20154-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Logan M, Martin JF, Nagy A, Lobe C, Olson EN, Tabin CJ
  TITLE     Expression of Cre Recombinase in the developing mouse limb bud 
            driven by a Prxl enhancer.
  JOURNAL   Genesis. 2002 Jun . 33(2):77-80.
  PUBMED    12112875
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2002 Jun . 33(2):77-80."
COMMENT     Sequence Label: pBS mPrx1 Cre (CT#644)
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..21
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6..22
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     28..47
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(356..376)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          506..571
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        655..673
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     655..672
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cccctcgagg tcgacggtat cgattgtgaa atttgtgatg ctattgcttt atttgtaacc
       61 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      121 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      181 gattatgatc tctagtcaag gcactataca tcaaatattt ttccataatt ttcttgtata
      241 gcagtgcagc tttttccttt gtggtgtaaa tagcaaagca agcaagagtt ctattactaa
      301 acacagcatg actcaaaaaa cttagcaatt ctgaaggaaa gtccttgggg tcttctacct
      361 ttctcttctt ttttggagga gtagaatgtt gagagtcagc agtagcctca tcatcactag
      421 atggcatttc ttctgagcaa aacaggtttt cctcattaaa ggcattccac cactgctccc
      481 attcatcagt tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa
      541 cacattatac acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt
      601 tgtccaatta tgtcacacca cagaagtaag gttccttcac aaagatcctc tagcatttag
      661 gtgacactat agaatagggc cctcaagctt gatatcgaat tctggctaat cgccatcttc
      721 cagcaggcgc accattgccc ctgtttcact atcca
//
