LOCUS       sequence-201546-        3625 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ompR from a TetR reuglated promoter under inducible ATC 
            control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Landry BP, Palanki R, Dyulgyarov N, Hartsough LA, Tabor JJ
  TITLE     Phosphatase activity tunes two-component system sensor detection 
            threshold.
  JOURNAL   Nat Commun. 2018 Apr 12;9(1):1433. doi: 10.1038/s41467-018-03929-y.
  PUBMED    29650958
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-04-12"; volume: "9(1):1433. doi"; pages: " 
            10.1038/s41467-018-03929-y"
COMMENT     Sequence Label: pSR26-2
FEATURES             Location/Qualifiers
     source          1..3625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(152..740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(232..251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(828..922)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(943..1602)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1517..1536
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1771..1789
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(1810..1828,1829..1834,1835..1851,1852..1857,
                     1858..1883)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1810..1828
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1835..1853
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      2659..2730
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2746..2773
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2825..2859
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     CDS             2894..3520
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(2970..2989)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      3526..3612
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cctagggcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
       61 ccacagaatc aggggataac gcatgcaaga acatgtgagc aaaaggccag caaaaggcca
      121 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      181 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      241 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      301 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      361 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      421 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      481 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      541 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
      601 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
      661 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
      721 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
      781 ggaacgaaaa ctcacgttaa gggattttgg tcatgactag tgcttggatt ctcaccaata
      841 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
      901 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
      961 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     1021 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     1081 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     1141 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     1201 gaaataggcc aggttttcac cataacacgc cacatcttgc gaatatatgt gtagaaactg
     1261 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     1321 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     1381 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     1441 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     1501 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     1561 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     1621 tgaaaatctc gataactcaa aaaatacgac cggtagtgat cttatttcat tatggtgaaa
     1681 gttggaacct cttacgggcc ggccaacgtc tcattttcgc cagatatcga cgtctaagaa
     1741 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
     1801 cacctcgagt ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag
     1861 cacatcagca ggacgcactg accgcataaa ggacttagca aagcccaatt ttaaacaaat
     1921 gcaagagaac tacaagattc tggtggtcga tgacgacatg cgcctgcgtg cgctgctgga
     1981 acgttatctc accgaacaag gcttccaggt tcgaagcgtc gctaatgcag aacagatgga
     2041 tcgcctgctg actcgtgaat ctttccatct tatggtactg gatttaatgt tacctggtga
     2101 agatggcttg tcgatttgcc gacgtcttcg tagtcagagc aacccgatgc cgatcattat
     2161 ggtgacggcg aaaggggaag aagtggaccg tatcgtaggc ctggagattg gcgctgacga
     2221 ctacattcca aaaccgttta acccgcgtga actgctggcc cgtatccgtg cggtgctgcg
     2281 tcgtcaggcg aacgaactgc caggcgcacc gtcacaggaa gaggcggtaa ttgctttcgg
     2341 taagttcaaa cttaacctcg gtacgcgcga aatgttccgc gaagacgagc cgatgccgct
     2401 caccagcggt gagtttgcgg tactgaaggc actggtcagc catccgcgtg agccgctctc
     2461 ccgcgataag ctgatgaacc ttgcccgtgg tcgtgaatat tccgcaatgg aacgctccat
     2521 cgacgtgcag atttcgcgtc tgcgccgcat ggtggaagaa gatccagcgc atccgcgtta
     2581 cattcagacc gtctggggtc tgggctacgt ctttgtaccg gacggctcta aagcatgata
     2641 ataatctaga ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2701 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2761 gggcctttct gcgtttatag gtacccgtgg atcctctaga gtcgacctgc aggcatgcaa
     2821 gctttttacg gctagctcag tcctaggtat agtgctagcc cagccagaga aacactcttt
     2881 aacagggggt agtatgatgt ctcgtttaga taaaagtaaa gtgattaaca gcgcattaga
     2941 gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg
     3001 tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt
     3061 agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg
     3121 gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga
     3181 tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa
     3241 tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag
     3301 cgcagtgggg cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa
     3361 agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga
     3421 attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg
     3481 cggattagaa aaacaactta aatgtgaaag tgggtcttaa ggcatcaaat aaaacgaaag
     3541 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     3601 agtaggacaa atccgccgcc ctaga
//
