LOCUS       sequence-201566-        5787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that 
            targets the FWA promoter in arabidopsis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo 
            HY, Zhao JM, Segal DJ, Jacobsen SE
  TITLE     Targeted DNA demethylation of the Arabidopsis genome using the human
            TET1 catalytic domain.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 
            10.1073/pnas.1716945115. Epub 2018 Feb 14.
  PUBMED    29444862
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1716945115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-02-27- 27"; volume: "115"; issue: "9"; pages: 
            "E2125-E2134"
COMMENT     Sequence Label: pUBQ10::ZF108_3xFLAG_TET1cd
FEATURES             Location/Qualifiers
     source          1..5787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2022..2066
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             2094..2117
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2628..2699
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    2739..2763
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4966..4990)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agtctagctc aacagagctt ttaacccaaa ttggtacaat agaatacaac tttagatcat
       61 aattctcaaa agaaagagat tccttagcta ttctatctgc cactccattt ccttctcggc
      121 ttgtatgcac aagcataaaa tcctcaaact tgctaagtag atactttatg tcttggataa
      181 ttggattgag acttgacaag cataactttc atgtaaccaa agacacaagt tgctgagaat
      241 ccacctcaaa aatgatcttc ctataattga atcgggataa tgacagcaca gcccatctaa
      301 gagcctccac ttctacttcc agcacgcttc ttacttttac cacagctctt gcacctaacc
      361 ataacacctt ccctgtatga tcgcgaagca cccaccctaa gccacatttt aatccttctg
      421 ttggccatgc cccatcaaag ttgcacttaa cccaagattg tggtggagct tcccatgttt
      481 ctcgtctgtc ccgacggtgt tgtggttggt gctttcctta cattctgagc ctctttcctt
      541 ctaatccact catctgcatc ttcttgtgtc cttactaata cctcattggt tccaaattcc
      601 ctccctttaa gcaccagctc gtttctgttc ttccacagcc tcccaagtat ccaagggact
      661 aaagcctcca cattcttcag atcaggatat tcttgtttaa gatgttgaac tctatggagg
      721 tttgtatgaa ctgatgatct aggaccggat aagttccctt cttcatagcg aacttattca
      781 aagaatgttt tgtgtatcat tcttgttaca ttgttattaa tgaaaaaata ttattggtca
      841 ttggactgaa cacgagtgtt aaatatggac caggccccaa ataagatcca ttgatatatg
      901 aattaaataa caagaataaa tcgagtcacc aaaccacttg ccttttttaa cgagacttgt
      961 tcaccaactt gatacaaaag tcattatcct atgcaaatca ataatcatac aaaaatatcc
     1021 aataacacta aaaaattaaa agaaatggat aatttcacaa tatgttatac gataaagaag
     1081 ttacttttcc aagaaattca ctgattttat aagcccactt gcattagata aatggcaaaa
     1141 aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta gaaaatacga aatacgcttc
     1201 aatgcagtgg gacccacggt tcaattattg ccaattttca gctccaccgt atatttaaaa
     1261 aataaaacga taatgctaaa aaaatataaa tcgtaacgat cgttaaatct caacggctgg
     1321 atcttatgac gaccgttaga aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa
     1381 gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct
     1441 caacctaaaa ataaggcaat tagccaaaaa caactttgcg tgtaaacaac gctcaataca
     1501 cgtgtcattt tattattagc tattgcttca ccgccttagc tttctcgtga cctagtcgtc
     1561 ctcgtctttt cttcttcttc ttctataaaa caatacccaa agagctcttc ttcttcacaa
     1621 ttcagatttc aatttctcaa aatcttaaaa actttctctc aattctctct accgtgatca
     1681 aggtaaattt ctgtgttcct tattctctca aaatcttcga ttttgttttc gttcgatccc
     1741 aatttcgtat atgttctttg gtttagattc tgttaatctt agatcgaaga cgattttctg
     1801 ggtttgatcg ttagatatca tcttaattct cgattagggt ttcatagata tcatccgatt
     1861 tgttcaaata atttgagttt tgtcgaataa ttactcttcg atttgtgatt tctatctaga
     1921 tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga ttaatctgag tttttctgat
     1981 taacagacgc gtccatggtt gggtaccaac aaatggctgg tggacttaac gatatcttcg
     2041 aagctcagaa gattgaatgg catgaggata ctggtggatc tagcatcccc ggacttgagg
     2101 ttcttttcca aggacctctc gagccttata aatgccctga gtgtggaaaa tctttctctc
     2161 aatctggaga tcttaggagg caccaaagga cccataccgg tgaaaaacct tataaatgcc
     2221 ctgagtgtgg aaaatcattc agtaggagtg atcatcttac cacccatcag aggacccata
     2281 ctggtgaaaa accttataaa tgccctgagt gtggaaaatc attctcacaa tcttcatctc
     2341 ttgttaggca tcagaggact cataccggag aaaaacctta taaatgccct gagtgtggaa
     2401 aatcattctc aacatctgga aaccttgtta ggcaccaaag gacccatact ggtgaaaaac
     2461 cttataaatg ccctgagtgt ggaaaatcat tctctcagag agcaaacctt agggctcatc
     2521 aaaggacaca cactggagaa aaaccttata aatgccctga gtgtggaaaa tctttctcta
     2581 ggagtgataa gctcaccgag catcaaagga ctcacaccgg actcgaggac tacaaagacg
     2641 atgacgacaa agactacaaa gacgatgacg acaaagacta caaagacgat gacgacaaaa
     2701 agcttgatat cgccatgggg ggcgcgccaa gctatcaaac aagtttgtac aaaaaagcag
     2761 gctccgcggc cgcccccttc accatgggat ccctgcccac ctgcagctgt cttgatcgag
     2821 ttatacaaaa agacaaaggc ccatattata cacaccttgg ggcaggacca agtgttgctg
     2881 ctgtcaggga aatcatggag aataggtatg gtcaaaaagg aaacgcaata aggatagaaa
     2941 tagtagtgta caccggtaaa gaagggaaaa gctctcatgg gtgtccaatt gctaagtggg
     3001 ttttaagaag aagcagtgat gaagaaaaag ttctttgttt ggtccggcag cgtacaggcc
     3061 accactgtcc aactgctgtg atggtggtgc tcatcatggt gtgggatggc atccctcttc
     3121 caatggccga ccggctatac acagagctca cagagaatct aaagtcatac aatgggcacc
     3181 ctaccgacag aagatgcacc ctcaatgaaa atcgtacctg tacatgtcaa ggaattgatc
     3241 cagagacttg tggagcttca ttctcttttg gctgttcatg gagtatgtac tttaatggct
     3301 gtaagtttgg tagaagccca agccccagaa gatttagaat tgatccaagc tctcccttac
     3361 atgaaaaaaa ccttgaagat aacttacaga gtttggctac acgattagct ccaatttata
     3421 agcagtatgc tccagtagct taccaaaatc aggtggaata tgaaaatgtt gcccgagaat
     3481 gtcggcttgg cagcaaggaa ggtcgaccct tctctggggt cactgcttgc ctggacttct
     3541 gtgctcatcc ccacagggac attcacaaca tgaataatgg aagcactgtg gtttgtacct
     3601 taactcgaga agataaccgc tctttgggtg ttattcctca agatgagcag ctccatgtgc
     3661 tacctcttta taagctttca gacacagatg agtttggctc caaggaagga atggaagcca
     3721 agatcaaatc tggggccatc gaggtcctgg caccccgccg caaaaaaaga acgtgtttca
     3781 ctcagcctgt tccccgttct ggaaagaaga gggctgcgat gatgacagag gttcttgcac
     3841 ataagataag ggcagtggaa aagaaaccta ttccccgaat caagcggaag aataactcaa
     3901 caacaacaaa caacagtaag ccttcgtcac tgccaacctt agggagtaac actgagaccg
     3961 tgcaacctga agtaaaaagt gaaaccgaac cccattttat cttaaaaagt tcagacaaca
     4021 ctaaaactta ttcgctgatg ccatccgctc ctcacccagt gaaagaggca tctccaggct
     4081 tctcctggtc cccgaagact gcttcagcca caccagctcc actgaagaat gacgcaacag
     4141 cctcatgcgg gttttcagaa agaagcagca ctccccactg tacgatgcct tcgggaagac
     4201 tcagtggtgc caatgctgca gctgctgatg gccctggcat ttcacagctt ggcgaagtgg
     4261 ctcctctccc caccctgtct gctcctgtga tggagcccct cattaattct gagccttcca
     4321 ctggtgtgac tgagccgcta acgcctcatc agccaaacca ccagccctcc ttcctcacct
     4381 ctcctcaaga ccttgcctct tctccaatgg aagaagatga gcagcattct gaagcagatg
     4441 agcctccatc agacgaaccc ctatctgatg accccctgtc acctgctgag gagaaattgc
     4501 cccacattga tgagtattgg tcagacagtg agcacatctt tttggatgca aatattggtg
     4561 gggtggccat cgcacctgct cacggctcgg ttttgattga gtgtgcccgg cgagagctgc
     4621 acgctaccac tcctgttgag caccccaacc gtaatcatcc aacccgcctc tcccttgtct
     4681 tttaccagca caaaaaccta aataagcccc aacatggttt tgaactaaac aagattaagt
     4741 ttgaggctaa agaagctaag aataagaaaa tgaaggcctc agagcaaaaa gaccaggcag
     4801 ctaatgaagg tccagaacag tcctctgaag taaatgaatt gaaccaaatt ccttctcata
     4861 aagcattaac attaacccat gacaatgttg tcaccgtgtc cccttatgct ctcacacacg
     4921 ttgcggggcc ctataaccat tgggtctgaa agggtgggcg cgccgaccca gctttcttgt
     4981 acaaagtggt tcgataattc cttaattaac tagttctaga gcggccgcca ccgcggtgga
     5041 gctcctaggt gagtctagag agttaattaa gacccgggac tagtccctag agtcctgctt
     5101 taatgagata tgcgagacgc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac
     5161 gttgtaaaaa acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta
     5221 atgaatatat cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact
     5281 gattgtaccc tactacttat atgtacaata ttaaaatgaa aacaatatat tgtgctgaat
     5341 aggtttatag cgacatctat gatagagcgc cacaataaca aacaattgcg ttttattatt
     5401 acaaatccaa ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact gattacataa
     5461 atcttattca aatttcaaaa gtgccccagg ggctagtatc tacgacacac cgagcggcga
     5521 actaataacg ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc
     5581 ttgaagttga gtattggccg tccgctctac cgaaagttac gggcaccatt caacccggtc
     5641 cagcacggcg gccgggtaac cgacttgctg ccccgagaat tatgcagcat ttttttggtg
     5701 tatgtgggcc ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg
     5761 ggtccagggc gaattttgcg acgagct
//
