LOCUS       sequence-201568-        9752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant expression vector for multigene cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Lukan T, Machens F, Coll A, Baebler S, Messerschmidt K, Gruden K
  TITLE     Plant X-tender: An extension of the AssemblX system for the assembly
            and expression of multigene constructs in plants.
  JOURNAL   PLoS One. 2018 Jan 4;13(1):e0190526. doi: 
            10.1371/journal.pone.0190526. eCollection 2018.
  PUBMED    29300787
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-01-4"; volume: "13(1):e0190526. doi"; pages: " 
            10.1371/journal.pone.0190526. eCollection 2018"
COMMENT     Sequence Label: pCAMBIA_ASX
FEATURES             Location/Qualifiers
     source          1..9752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             155..460
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     339..358
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(567..584)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(567..583)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(576..598)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    786..810
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2111..2740
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3169..4242
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4308..4502
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4738..4760)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4846..4986
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4901..4920
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5001..5018)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5172..5760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5252..5271)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5847..6641)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7066..7090
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    7168..7342
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7383..8408)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8475..9151)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(8518..8538)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    9342..9363
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9378..9383,9384..9401,9402..9408)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9416..9432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9421..9443
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9440..9456
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9440..9456
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
       61 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
      121 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
      181 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
      241 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga
      301 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
      361 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
      421 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
      481 cagccagtaa gctttaaggt ttcagtcaca ctacggatac ttttacaacg ggagcagtta
      541 ttcaattacc ctgttatccc tacggcactg gccgtcgttt tacaacgtcg tgactgggaa
      601 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      661 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      721 tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca
      781 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
      841 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
      901 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
      961 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
     1021 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     1081 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     1141 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     1201 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     1261 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     1321 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     1381 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     1441 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     1501 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     1561 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     1621 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     1681 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     1741 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     1801 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     1861 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg
     1921 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     1981 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     2041 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
     2101 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     2161 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     2221 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     2281 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     2341 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     2401 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     2461 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     2521 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     2581 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     2641 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     2701 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     2761 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     2821 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     2881 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     2941 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     3001 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     3061 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc
     3121 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc
     3181 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg
     3241 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg
     3301 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg
     3361 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc
     3421 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg
     3481 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag
     3541 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg
     3601 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc
     3661 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga
     3721 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg
     3781 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg
     3841 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag
     3901 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt
     3961 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc
     4021 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc
     4081 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac
     4141 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc
     4201 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta
     4261 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg
     4321 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag
     4381 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat
     4441 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca
     4501 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg
     4561 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg
     4621 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac
     4681 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     4741 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     4801 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     4861 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     4921 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     4981 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5041 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5101 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5161 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5221 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5281 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5341 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5401 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5461 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5521 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5581 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     5641 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5701 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5761 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5821 gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt
     5881 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct
     5941 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc
     6001 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg
     6061 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa
     6121 aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc
     6181 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta
     6241 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac
     6301 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca
     6361 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt
     6421 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag
     6481 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc
     6541 agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg
     6601 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt
     6661 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac
     6721 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa
     6781 agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag
     6841 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
     6901 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
     6961 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg
     7021 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
     7081 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta
     7141 ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag
     7201 gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact aagggtttct
     7261 tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta
     7321 ctcacacatt attatggaga aactcgagct tgtcgatcga cagatcccgg tcggcatcta
     7381 ctctatttct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
     7441 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
     7501 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
     7561 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
     7621 cggagtcgtg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
     7681 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
     7741 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
     7801 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
     7861 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
     7921 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
     7981 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
     8041 gcgatcgcat ccatagcctc cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc
     8101 aggtcttgca acgtgacacc ctgtgaacgg cgggagatgc aataggtcag gctctcgcta
     8161 aactccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
     8221 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
     8281 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
     8341 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
     8401 tttttcatat ctcattgccc cccggatctg cgaaagctcg agagagatag atttgtagag
     8461 agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa
     8521 gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat
     8581 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
     8641 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
     8701 ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag
     8761 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
     8821 gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
     8881 gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     8941 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     9001 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     9061 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     9121 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     9181 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag
     9241 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     9301 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     9361 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     9421 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc gagctcggta
     9481 cccggtaggg ataacagggt aatatcccta ttgtcccatt aaaataggaa agtctggtaa
     9541 ctcaggacac tgtatctgct acgctgttta taagcttggc ttactaaaag ccagataaca
     9601 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg
     9661 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac
     9721 agctatcagt tgctcaaggc atatatgatg tc
//
