LOCUS       sequence-201596-        3693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HLA-B0702 his tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Lampen MH, Uchtenhagen H, Blom K, Varnaite R, Pakalniene J, 
            Dailidyte L, Walchli S, Lindquist L, Mickiene A, Michaelsson J, 
            Schumacher TN, Ljunggren HG, Sandberg JK, Achour A, Gredmark-Russ S
  TITLE     Breadth and Dynamics of HLA-A2- and HLA-B7-Restricted CD8(+) T Cell 
            Responses against Nonstructural Viral Proteins in Acute Human 
            Tick-Borne Encephalitis Virus Infection.
  JOURNAL   Immunohorizons. 2018 Jul 2;2(6):172-184. doi: 
            10.4049/immunohorizons.1800029.
  PUBMED    31022685
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/immunohorizons"; journalName: "Immunohorizons"; date: 
            "2018-07-2- 2"; volume: "2"; issue: "6"; pages: "172-184"
COMMENT     Sequence Label: pENTR-HLA-B0702-His
FEATURES             Location/Qualifiers
     source          1..3693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     56..75
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     309..326
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(485..512)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(604..690)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     739..761
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     753..770
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     754..770
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    786..885
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1992..2009
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(2035..2134)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2151..2170)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2152..2170)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2175..2191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2175..2191)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2293..2312)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2304..3113
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2371..2390)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(3260..3693,1..155)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
       61 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      121 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      181 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      241 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
      301 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
      361 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      421 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
      481 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
      541 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
      601 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
      661 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
      721 cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg
      781 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg
      841 agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggctccgcg gccgccccct
      901 tcaccatgct ggtcatggcg ccccgaaccg tcctcctgct gctctcggcg gccctggccc
      961 tgaccgagac ctgggccggc tcccactcca tgaggtattt ctacacctcc gtgtcccggc
     1021 ccggccgcgg ggagccccgc ttcatctcag tgggctacgt ggacgacacc cagttcgtga
     1081 ggttcgacag cgacgccgcg agtccgagag aggagccgcg ggcgccgtgg atagagcagg
     1141 aggggccgga gtattgggac cggaacacac agatctacaa ggcccaggca cagactgacc
     1201 gagagagcct gcggaacctg cgcggctact acaaccagag cgaggccggg tctcacaccc
     1261 tccagagcat gtacggctgc gacgtggggc cggacgggcg cctcctccgc gggcatgacc
     1321 agtacgccta cgacggcaag gattacatcg ccctgaacga ggacctgcgc tcctggaccg
     1381 ccgcggacac ggcggctcag atcacccagc gcaagtggga ggcggcccgt gaggcggagc
     1441 agcggagagc ctacctggag ggcgagtgcg tggagtggct ccgcagatac ctggagaacg
     1501 ggaaggacaa gctggagcgc gctgaccccc caaagacaca cgtgacccac caccccatct
     1561 ctgaccatga ggccaccctg aggtgctggg ccctgggttt ctaccctgcg gagatcacac
     1621 tgacctggca gcgggatggc gaggaccaaa ctcaggacac tgagcttgtg gagaccagac
     1681 cagcaggaga tagaaccttc cagaagtggg cagctgtggt ggtgccttct ggagaagagc
     1741 agagatacac atgccatgta cagcatgagg ggctgccgaa gcccctcacc ctgagatggg
     1801 agccgtcttc ccagtccacc gtccccatcg tgggcattgt tgctggcctg gctgtcctag
     1861 cagttgtggt catcggagct gtggtcgctg ctgtgatgtg taggaggaag agttcaggtg
     1921 gaaaaggagg gagctactct caggctgcgt gcagcgacag tgcccagggc tctgatgtgt
     1981 ctctcacagc tcatcaccat caccatcatt gactcgagaa gggtgggcgc gccgacccag
     2041 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     2101 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     2161 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     2221 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     2281 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     2341 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     2401 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     2461 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     2521 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     2581 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     2641 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     2701 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     2761 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     2821 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     2881 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     2941 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     3001 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     3061 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
     3121 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct
     3181 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     3241 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3301 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3361 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3421 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3481 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3541 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3601 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3661 gcgccacgct tcccgaaggg agaaaggcgg aca
//
