LOCUS       sequence-201598-        6749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HLA-B0702 his tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Lampen MH, Uchtenhagen H, Blom K, Varnaite R, Pakalniene J, 
            Dailidyte L, Walchli S, Lindquist L, Mickiene A, Michaelsson J, 
            Schumacher TN, Ljunggren HG, Sandberg JK, Achour A, Gredmark-Russ S
  TITLE     Breadth and Dynamics of HLA-A2- and HLA-B7-Restricted CD8(+) T Cell 
            Responses against Nonstructural Viral Proteins in Acute Human 
            Tick-Borne Encephalitis Virus Infection.
  JOURNAL   Immunohorizons. 2018 Jul 2;2(6):172-184. doi: 
            10.4049/immunohorizons.1800029.
  PUBMED    31022685
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/immunohorizons"; journalName: "Immunohorizons"; date: 
            "2018-07-2- 2"; volume: "2"; issue: "6"; pages: "172-184"
COMMENT     Sequence Label: pMP71-HLA-B0702-His
FEATURES             Location/Qualifiers
     source          1..6749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(448..465)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(448..464)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(457..479)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(673..692)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     792..814
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(852..870)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        938..1042
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1043..1111,1112..1903)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1261..1280)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2074..2662
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2816..2833
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2950..2971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2986..2991,2992..3009,3010..3016)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3024..3040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3029..3051
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3048..3064
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3048..3064
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             3089..3679
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4185..4588
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     protein_bind    4833..4857
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4883..4900)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(6007..6031)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6062..6081)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6063..6081)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtctatcgga ggactggcgc gccgagtgag gggttgtggg ctcttttatt gagctcgggg
       61 agcagaagcg cgcgaacaga agcgagaagc gaactgattg gttagttcaa ataaggcaca
      121 gggtcatttc aggtccttgg ggcaccctgg aaacatctga tggttctcta gaaactgctg
      181 agggcgggac cgcatctggg gaccatctgt tcttggccct gagccggggc aggaactgct
      241 taccacagat atcctgtttg gcccatattc tgctgttcca actgttcttg gccctgagcc
      301 ggggcaggaa ctgcttacca cagatatcct gtttggccca tattctgctg tctctctgtt
      361 cctaaccttg atctgaactt ctctattctc agttatgtat tttccatgcc ttgcaaaatg
      421 gcgttactta agctagagta ctaattcact ggccgtcgtt ttacaacgtc gtgactggga
      481 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      541 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      601 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
      661 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
      721 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
      781 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
      841 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      901 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      961 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1021 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1081 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1141 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1201 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1261 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     1321 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1381 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1441 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1501 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1561 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1621 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1681 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1741 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1801 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1861 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1921 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1981 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2041 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2101 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2161 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2221 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2281 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2341 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2401 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2461 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2521 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggcgaaacg cctggtatct
     2581 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2641 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2701 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2761 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     2821 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     2881 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     2941 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     3001 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     3061 tgaccatgat tacgccaagc tgctcgagaa tgaaagaccc ccgaggtggg cagtcaatca
     3121 ctcagaggag accctcccaa ggaacagcga gaccacgagt cggatgcaac tgcaagaggg
     3181 tttattgaga acacgggtac ccgggcgacg cagtctatcg gaggactggc gcgccgagtg
     3241 aggggttgtg ggctctttta ttgagctcgg ggagcagaag cgcgcgaaca gaagcgagaa
     3301 gcgaactgat tggttagttc aaataaggca cagggtcatt tcaggtcctt ggggcaccct
     3361 ggaaacatct gatggttctc tagaaactgc tgagggcggg accgcatctg gggaccatct
     3421 gttcttggcc ctgagccggg gcaggaactg cttaccacag atatcctgtt tggcccatat
     3481 tctgctgttc caactgttct tggccctgag ccggggcagg aactgcttac cacagatatc
     3541 ctgtttggcc catattctgc tgtctctctg ttcctaacct tgatctgaac ttctctattc
     3601 tcagttatgt attttccatg ccttgcaaaa tggcgttact taagctagct tgccaaacct
     3661 acaggtgggg tctttcattc cccccttttt ctggagacta aataaaatct tttattctat
     3721 ctatggctcg tgttaagctt agcgctaggc agaatccaga tgctcaaggc ccttcataat
     3781 atcccccagt ttagtagttg gacttaggga acaaaggaac ctttaataga aatgggacag
     3841 caagaaagcg agcaggccta agcttggatc cgaattattc ttggcatgcc aagttgacga
     3901 tttgcatgcc aggagacaaa gttcacggtg gaatccatgg ttcgcaattc tgcacaggtg
     3961 acgaccaagc aacacggacc ggacgccgag gcgaaacagg cggggaggcg gcccaaaggg
     4021 agatccgact cgtcggaggc cgaaagcgaa gacgcgggag aggccgcaga accggcagaa
     4081 ggcctcggga agggaggtcc gctggattga gagccgaagg gacgtagcag aaggacgtcc
     4141 cgcgcaggat ccagttggca acacaggcga gcagccatgg aaaggacgtc agcttccccg
     4201 acaacaccac ggaattatca gtgcccagca acctagcccc tgtccagcag cgggcaaggc
     4261 aggcggcgat gagttctgcc gtggcgatcg ggagggggaa agcgaaagtc ccagaaagga
     4321 gttgacaggt ggtggcaatg ccccagccag tgggggttgc gtcagcaaac acagagcaca
     4381 ccacgccacg ttgacggaca acgggccaca actcctcata aagagacagc aaccaggatt
     4441 tatacaagga ggagaaaacg aaagccgtac gggaagcaat agcatgatac agaggcatta
     4501 aagcagcata tccacacagc gtaaaaggag caacatagtt aagaatatca gtcaatcttt
     4561 cacaaatttt gtaatccaga ggttgattaa caggaacaga gcgtaaataa cgggaaagtt
     4621 tcttaacatg tttgtcttgt ggcaatacac ctgaactagt aattacatat cccataaaat
     4681 gtaaatgatt gccccaccat tttgttttat taacatttaa atgtataccc aaatcaagaa
     4741 aaacagaaca aatatgggaa taaatggcgg taagatgctc gaattgaggg ttagggatag
     4801 gcttaccttc gaaccgcggg ccctctagat caaccacttt gtacaagaaa gctgggtcgg
     4861 cgcgcccacc cttctcgagt caatgatggt gatggtgatg agctgtgaga gacacatcag
     4921 agccctgggc actgtcgctg cacgcagcct gagagtagct ccctcctttt ccacctgaac
     4981 tcttcctcct acacatcaca gcagcgacca cagctccgat gaccacaact gctaggacag
     5041 ccaggccagc aacaatgccc acgatgggga cggtggactg ggaagacggc tcccatctca
     5101 gggtgagggg cttcggcagc ccctcatgct gtacatggca tgtgtatctc tgctcttctc
     5161 cagaaggcac caccacagct gcccacttct ggaaggttct atctcctgct ggtctggtct
     5221 ccacaagctc agtgtcctga gtttggtcct cgccatcccg ctgccaggtc agtgtgatct
     5281 ccgcagggta gaaacccagg gcccagcacc tcagggtggc ctcatggtca gagatggggt
     5341 ggtgggtcac gtgtgtcttt ggggggtcag cgcgctccag cttgtccttc ccgttctcca
     5401 ggtatctgcg gagccactcc acgcactcgc cctccaggta ggctctccgc tgctccgcct
     5461 cacgggccgc ctcccacttg cgctgggtga tctgagccgc cgtgtccgcg gcggtccagg
     5521 agcgcaggtc ctcgttcagg gcgatgtaat ccttgccgtc gtaggcgtac tggtcatgcc
     5581 cgcggaggag gcgcccgtcc ggccccacgt cgcagccgta catgctctgg agggtgtgag
     5641 acccggcctc gctctggttg tagtagccgc gcaggttccg caggctctct cggtcagtct
     5701 gtgcctgggc cttgtagatc tgtgtgttcc ggtcccaata ctccggcccc tcctgctcta
     5761 tccacggcgc ccgcggctcc tctctcggac tcgcggcgtc gctgtcgaac ctcacgaact
     5821 gggtgtcgtc cacgtagccc actgagatga agcggggctc cccgcggccg ggccgggaca
     5881 cggaggtgta gaaatacctc atggagtggg agccggccca ggtctcggtc agggccaggg
     5941 ccgccgagag cagcaggagg acggttcggg gcgccatgac cagcatggtg aagggggcgg
     6001 ccgcggagcc tgcttttttg tacaaacttg ttgatcaagc ttacctagcc agcttgggtc
     6061 tccctatagt gagtcgtatt actcgaggcc gcctgtaagt gagcttggag agagggacta
     6121 ttacttaact ttgtcgagct aattttcaga caaatacaga aacacagtca gacagagaca
     6181 acacagaacg atgctgcagc agacaagacg cgcggcgcgg tttcggcgta aaaccgaaag
     6241 caaaaattca gacgggggcg ggaagtgtct ctgtctctcg tcccccaaca aagccacgta
     6301 cccctcggac agtggcggag ctccaaaaag tccgactcgg gtaggttaac tgatacagaa
     6361 tgggccacaa aacgggcccc cgaggccgct gggacgtctc ccaggggctg cggccgggaa
     6421 tacgaactcg tcagttcttc cgcgggaccg ccagatacag atctagttag ccaactagta
     6481 cagacgcagg cgcaaacatt agatgccggc acaaacacgc acaaagacag agacagacac
     6541 gaaacgaccg ctggccagct tacctcccgg cgggggggtc ggtggtccct gggcaggggt
     6601 ctccaaatct cggtggaacc tccaaatgaa agacccccga ggtgggcagt caatcaatct
     6661 gaggagaccc tcccaaggat cagcgagtcc acgattcgga tgcaaacagc aagaggcttt
     6721 attgggaata cgggtacccg ggcgacgca
//
